Methanohalophilus zhilinae sp. nov., an Alkaliphilic, Halophilic, Methylotrophic Methanogen

Mathrani, Indra M.; Boone, David R.; Mah, Robert A.; Fox, George E.; Lau, Paul P.

doi:10.1099/00207713-38-2-139

Cited by 148 publications

(31 citation statements)

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“…The results clearly demonstrated a dominance of methylotrophic methanogenesis and absence of acetoclastic processes, while the results concerning hydrogenotrophic methanogenesis were inconclusive (Oremland and Miller 1993; Namsaraev et al 1999; Sorokin et al 2004a; Nolla-Ardèvol et al 2012). Some of the key haloalkaliphilic players in soda lake methanogenesis have been isolated in pure culture and described, including two groups of methylotrophs, such as Methanolobus taylorii (moderate salinity) and Methanosalsum zhilinae (high salinity), and a highly salt-tolerant lithotroph Methanocalculus natronophilus (Mathrani et al 1988; Oremland and Boone 1994; Kevbrin et al 1997; Zhilina et al 2013). …”

Section: Cultured Diversity and Their Role In Biogeochemical Cyclesmentioning

confidence: 99%

“…alkalicus Kunkur Steppe (Siberia, Russia)Sorokin et al (1998) NA Halomonas H. mongoliensis Lake Dzun-Tukhem-Nur (Mongolia)Boltyanskaya et al (2007)NA H. kenyensis L. Bogoria, L. Nakuru, L. Elmentieta, Crater Lake, L. Magadi (Kenya)Boltyanskaya et al (2007)NA Methanohalophilus (= Methanosalsum) M. zhilinae Bosa Lake (Wadi Natrun, Egypt)Mathrani et al (1988) Complete2138208339 Methylomicrobium Mm. buryatense Lake Khadyn (Siberia, Russia)Kaluzhnaya et al (2001); Sorokin et al (2000)PD5067453049 Mm.…”

Section: Introductionmentioning

confidence: 99%

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Microbial diversity and biogeochemical cycling in soda lakes

et al. 2014

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Soda lakes contain high concentrations of sodium carbonates resulting in a stable elevated pH, which provide a unique habitat to a rich diversity of haloalkaliphilic bacteria and archaea. Both cultivation-dependent and -independent methods have aided the identification of key processes and genes in the microbially mediated carbon, nitrogen, and sulfur biogeochemical cycles in soda lakes. In order to survive in this extreme environment, haloalkaliphiles have developed various bioenergetic and structural adaptations to maintain pH homeostasis and intracellular osmotic pressure. The cultivation of a handful of strains has led to the isolation of a number of extremozymes, which allow the cell to perform enzymatic reactions at these extreme conditions. These enzymes potentially contribute to biotechnological applications. In addition, microbial species active in the sulfur cycle can be used for sulfur remediation purposes. Future research should combine both innovative culture methods and state-of-the-art ‘meta-omic’ techniques to gain a comprehensive understanding of the microbes that flourish in these extreme environments and the processes they mediate. Coupling the biogeochemical C, N, and S cycles and identifying where each process takes place on a spatial and temporal scale could unravel the interspecies relationships and thereby reveal more about the ecosystem dynamics of these enigmatic extreme environments.

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Section: Cultured Diversity and Their Role In Biogeochemical Cyclesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial diversity and biogeochemical cycling in soda lakes

et al. 2014

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“…A telling example is provided by M. zhilinae ( alias Methanohalophilus zhilinae ). Its optimal growth occurs at pH 9.2 and 45 °C [41], conditions in which the NH 3 /NH 4 + equilibrium is shifted toward NH 3 . It is possible that in M. zhilinae the excretion of toxic NH 3 could be facilitated by Rh50 and/or Amt, similar to the role suggested for the Rh50 proteins in the tilapia fish living in the alkaline waters (pH ~ 10) of Lake Magadi [42].…”

Section: Discussionmentioning

confidence: 99%

Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes

Matassi

2017

BMC Evol Biol

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BackgroundRh50 proteins belong to the family of ammonia permeases together with their Amt/MEP homologs. Ammonia permeases increase the permeability of NH3/NH4 + across cell membranes and are believed to be involved in excretion of toxic ammonia and in the maintenance of pH homeostasis. RH50 genes are widespread in eukaryotes but absent in land plants and fungi, and remarkably rare in prokaryotes. The evolutionary history of RH50 genes in prokaryotes is just beginning to be unveiled.ResultsHere, a molecular phylogenetic approach suggests horizontal gene transfer (HGT) as a primary force driving the evolution and spread of RH50 among prokaryotes. In addition, the taxonomic distribution of the RH50 gene among prokaryotes turned out to be very narrow; a single-copy RH50 is present in the genome of only a small proportion of Bacteria, and, first evidence to date, in only three methanogens among Euryarchaea. The coexistence of RH50 and AMT in prokaryotes seems also a rare event. Finally, phylogenetic analyses were used to reconstruct the HGT network along which prokaryotic RH50 evolution has taken place.ConclusionsThe eukaryotic or bacterial “origin” of the RH50 gene remains unsolved. The RH50 prokaryotic HGT network suggests a preferential directionality of transfer from aerobic to anaerobic organisms. The observed HGT events between archaeal methanogens, anaerobic and aerobic ammonia-oxidizing bacteria suggest that syntrophic relationships play a major role in the structuring of the network, and point to oxygen minimum zones as an ecological niche that might be of crucial importance for HGT-driven evolution.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0850-6) contains supplementary material, which is available to authorized users.

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“…[8][9][10] At the present time the hydrogen limits for an number of different groups of secondary hydrogenconsuming lithotrophic microorganisms are known:…”

Section: Competitors For Hydrogen In Anaerobic Microbial Communities mentioning

confidence: 99%

Potential application of anaerobic extremophiles for hydrogen production

Pikuta

Hoover

2004

Instruments, Methods, and Missions for Astrobiology VIII

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In processes of the substrate fermentation most anaerobes produce molecular hydrogen as a waste end product, which often controls the culture growth as an inhibitor. Usually in nature the hydrogen is easily removed from an ecosystem, due to its physical features, and an immediate consumption by the secondary anaerobes that sometimes behave as competitors for electron donors; a classical example of this kind of substrate competition in anaerobic microbial communities is the interaction between methanogens and sulfate-or sulfur-reducers. Previously, on the mixed cultures of anaerobes at neutral pH, it was demonstrated that bacterial hydrogen production could provide a good alternative energy source. At neutral pH the original cultures could easily contaminated by methanogens, and the most unpleasant side effect of these conditions is the development of pathogenic bacteria. In both cases the rate of hydrogen production was dramatically decreased since some part of the hydrogen was transformed to methane, and furthermore, the cultivation with pathogenic contaminants on an industrial scale would create an unsafe situation. In our laboratory the experiments with obligately alkaliphilic bacteria producing hydrogen as an end metabolic product were performed at different conditions. The mesophilic, haloalkaliphilic and obligately anaerobic bacterium Spirochaeta americana ASpG1 T was studied and various cultivation regimes were compared for the most effective hydrogen production. In a highly mineralized media with pH 9.5-10.0 not many known methanogens are capable of growth, and the probability of developing pathogenic contaminants is theoretically is close to zero (in medicine carbonate-saturated solutions are applied as antiseptics). Therefore the cultivation of alkaliphilic hydrogen producing bacteria could be considered as a safe and economical process for large-scale industrial bio-hydrogen production in the future. Here we present and discuss the experimental data with the rates of hydrogen productivity for S. americana ASpG1 isolated from soda Mono Lake in California.

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Methanohalophilus zhilinae sp. nov., an Alkaliphilic, Halophilic, Methylotrophic Methanogen

Cited by 148 publications

References 19 publications

Microbial diversity and biogeochemical cycling in soda lakes

Microbial diversity and biogeochemical cycling in soda lakes

Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes

Potential application of anaerobic extremophiles for hydrogen production

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