Methanol assimilation by yeasts

Hazeu, W.; Bruyn, Johanna C. de; Bos, P.

doi:10.1007/bf00425000

Cited by 47 publications

(19 citation statements)

References 8 publications

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“…Antigens immunologically related to those of Hansenula wickerhamii were detected only in the culture liquids of H, henricii, H. wickerhamii and Pichia pinus. All three species assimilate methanol (25) and hence are physiologically related. The genera Hansenula and Pichia are closely related (14).…”

Section: Resultsmentioning

confidence: 99%

Species-specific extracellular antigen production by ascomycetous yeasts, detected by ELISA.

Middelhoven

Notermans²

1988

J. Gen. Appl. Microbiol.

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Section: Resultsmentioning

confidence: 99%

Species-specific extracellular antigen production by ascomycetous yeasts, detected by ELISA.

Middelhoven

Notermans²

1988

J. Gen. Appl. Microbiol.

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“…The ability of yeast to utilize methanol as a sole carbon source for growth has been determined for genera such as Hansenula, Candida, Torulopsis, and Pichia (20). The metabolic pathway for the conversion of methanol to CO2 and H20 appears to be similar for several of these yeasts (2,24,30).…”

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confidence: 99%

“…IM1 minimal medium contains 36 mM KH2PO4, 23 mM (NH4)2SO4, 2 mM MgSO4, 6.7 mM KCI, 0.7 mM CaC12, 0.2 ,uM CUSO4 -5H20, 1.25 ,uM KI, 4.5 ,uM MnSO4, 2 ,uM Na2MoO4, 0.75 ,uM H3BO3, 17.5 ,uM ZnSO4, 44.5 ,uM FeCl2, and 1.6 ,uM Biotin. The cells grown on methanol were grown at a cell density of 140 g/liter (dry weight) with a retention time of 12 h. The cells grown on ethanol were grown at a cell density of 90 g/liter with a retention time of 11.5 h. When ethanol or methanol was used in a fermentor, feed stocks containing concentrations of 20 and 45% alcohol, respectively, were used.…”

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Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

Ellis¹,

Brust²,

Koutz³

et al. 1985

Mol. Cell. Biol.

247

125

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The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

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“…Since OGATA et al (1)(2)(3) first reported the assimilation of methanol by yeast as a sole source of carbon, many methanol-assimilating yeast strains have been isolated from natural sources or found in stock culture collections (4)(5)(6)(7)(8)(9)(10)(11)(12).…”

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New Species of Methanol-Assimilating Yeasts

Asai

Makiguchi

Shimada

et al. 1976

J. Gen. Appl. Microbiol.

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In the course of investigations on yeast cell production and on the metabolisms of methanol, two new species, Torulopsis nagoyaensis and Hansenula ofunaensis, were found in the yeasts isolated from various natural sources in Japan. Taxonomical studies of these new species are described.Since OGATA et al. (1-3) first reported the assimilation of methanol by yeast as a sole source of carbon, many methanol-assimilating yeast strains have been isolated from natural sources or found in stock culture collections (4-12).We isolated many yeast strains which can grow well at 37° in methanol medium. Among them, 3 strains could not be identified with any known species described in the literature. Two of these strains belonged to the same species of the genus Torulopsis and the other to the genus Hansenula. This paper deals with the descriptions of these two new species. MATERIALS AND METHODSMicroorganisms. Yeast strains, KM-1359, KM-1722, and KM-1833, were used in this study. They were isolated as yeasts assimilating methanol strongly from natural sources by methanol-enrichment culture method.Determination of yeasts. The experimental methods were mainly those described by LODDER and KREGER-VAN RI7 (13), LODDER et al. (14), and IIZUKA and GoTo (15). RESULTS AND DISCUSSIONTwo strains, KM-1722 and KM-1833, reproduced by multilateral budding and did not form ballistospores, ascospores, teliospores, endospores, or arthrospores. The vegetative cells were not triangular or "ogival." Pseudomycelia were not formed. They did not produce a starch-like compound or strong acetic acid from 197

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Methanol assimilation by yeasts

Cited by 47 publications

References 8 publications

Species-specific extracellular antigen production by ascomycetous yeasts, detected by ELISA.

Species-specific extracellular antigen production by ascomycetous yeasts, detected by ELISA.

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

New Species of Methanol-Assimilating Yeasts

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