Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Sarwar, Arslan; Lee, Eun Yeol

doi:10.1016/j.synbio.2023.06.001

Cited by 22 publications

(9 citation statements)

References 142 publications

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“…Although P. pastoris has been widely used as a cell factory for the production of a variety of compounds, − engineering the overproduction of complex secondary metabolites, such as isoprenoids, from sole methanol remains challenging. Here, we comprehensively optimized the MVA pathway in the peroxisome and cytosol for the overproduction of sesquiterpene.…”

Section: Discussionmentioning

confidence: 99%

Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis

Gao,

Hou,

Cai

et al. 2024

JACS Au

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Microbial metabolic engineering provides a feasible approach to sustainably produce advanced biofuels and biochemicals from renewable feedstocks. Methanol is an ideal feedstock since it can be massively produced from CO 2 through green energy, such as solar energy. However, engineering microbes to transform methanol and overproduce chemicals is challenging. Notably, the microbial production of isoprenoids from methanol is still rarely reported. Here, we extensively engineered Pichia pastoris (syn. Komagataella phaffii) for the overproduction of sesquiterpene αbisabolene from sole methanol by optimizing the mevalonate pathway and peroxisomal compartmentalization. Furthermore, through label-free quantification (LFQ) proteomic analysis of the engineered strains, we identified the key bottlenecks in the peroxisomal targeting pathway, and overexpressing the limiting enzyme EfmvaE significantly improved α-bisabolene production to 212 mg/L with the peroxisomal pathway. The engineered strain LH122 with the optimized peroxisomal pathway produced 1.1 g/ L α-bisabolene under fed-batch fermentation in shake flasks, achieving a 69% increase over that of the cytosolic pathway. This study provides a viable approach for overproducing isoprenoid from sole methanol in engineered yeast cell factories and shows that proteomic analysis can help optimize the organelle compartmentalized pathways to enhance chemical production.

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Section: Discussionmentioning

confidence: 99%

Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis

Gao,

Hou,

Cai

et al. 2024

JACS Au

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“…In addition, acetate ions are more stable and could be produced in media compatible with the following microbial steps, differently from formaldehyde. Methanol could be used but must be then in situ converted to formaldehyde, 177 as commented below. However, metabolic engineering will likely enlarge the range of suitable carbon source substrates derived electrocatalytically from CO 2 (CO, methane, formate, methanol acetate and ethanol) 178 suitable for producing sustainable foods from the air.…”

Section: Sustainable Food From the Airmentioning

confidence: 99%

Making chemicals from the air: the new frontier for hybrid electrosyntheses in artificial tree-like devices

Centi,

Perathoner

2024

Green Chem.

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“…A sustainable bioeconomy is one that includes a stronger focus on sustainability, including energy utilization, reduced greenhouse gas emissions, more efficient water utilization, and overall a transformative change in resource production and consumption to a more environmentally focused alternative to the current fossil-based economy ( Antar et al, 2021 ). The bioeconomy presently utilizes a diverse range of production organisms, encompassing bacteria, yeast, fungi, plants, and mammalian cells ( Table 1 ; Antranikian and Streit, 2022 ; Hankamer et al, 2023 ; Jo et al, 2023 ; Kamaludin and Feisal, 2023 ; Navarrete and Martínez, 2020 ; Nguyen and Lee, 2021 ; Sarwar and Lee, 2023 ; Soong et al, 2023 ; Zhang et al, 2017 ).…”

Section: Microalgae For Sustainable Bioeconomymentioning

confidence: 99%

Harnessing genetic engineering to drive economic bioproduct production in algae

Gupta,

Kang,

Pathania

et al. 2024

Front. Bioeng. Biotechnol.

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Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet the demands of a burgeoning population amidst climate-driven agricultural challenges. Microalgae emerge as a beacon of hope, offering a sustainable and renewable source of food, animal feed, and energy. Their rapid growth rates, adaptability to non-arable land and non-potable water, and diverse bioproduct range, encompassing biofuels and nutraceuticals, position them as a cornerstone of future resource management. Furthermore, microalgae’s ability to capture carbon aligns with environmental conservation goals. While microalgae offers significant benefits, obstacles in cost-effective biomass production persist, which curtails broader application. This review examines microalgae compared to other host platforms, highlighting current innovative approaches aimed at overcoming existing barriers. These approaches include a range of techniques, from gene editing, synthetic promoters, and mutagenesis to selective breeding and metabolic engineering through transcription factors.

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Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs

Cited by 22 publications

References 142 publications

Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis

Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis

Making chemicals from the air: the new frontier for hybrid electrosyntheses in artificial tree-like devices

Harnessing genetic engineering to drive economic bioproduct production in algae

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