Methanol Extract of Mongolian Iris bungei Maxim. Stimulates 3T3-L1 Adipocyte Differentiation

Abstract

Iris bungei Maxim. (IB), which is native to China and Mongolia, is used as a traditional medicine for conditions such as inflammation, cancer, and bacterial infections. However, the effects of Iris bungei Maxim. on adipocyte differentiation have not been studied. In the present study, we first demonstrated the molecular mechanisms underlying the adipogenic activity of the methanol extract of Mongolian I. bungei Maxim. (IB). IB significantly enhanced intracellular lipid accumulation and adipocyte differentiation in 3T3-L1 preadipocytes in a concentration-dependent manner. Moreover, IB markedly stimulated the expression of genes related to adipogenesis such as peroxisome proliferator-activated receptor γ, adiponectin, and aP2. In addition, we also observed that IB induces lipogenic genes such as fatty acid synthase, sterol regulatory element binding protein 1c, stearoyl-CoA desaturase, and acetyl-CoA carboxylase. Interestingly IB regulated adipocyte differentiation in both the early and middle stages. Taken together, these adipogenic and lipogenic effects of IB suggest its efficacy for the prevention and/or treatment of type 2 diabetes.

Stellera chamaejasme L. extract inhibits adipocyte differentiation through activation of the extracellular signal-regulated kinase pathway

“…After 40 cycles, the specificity of the PCR reaction was confirmed through melting curve analysis and gene expression was quantified by comparing CT values with those of standard 36B4 gene. Most of primer sequences used in the current experiments had been used in our previous study [ 24 ]. The sequences of the remaining primers are as follows: CDK2, (F) and (R) ; CDK4, (F) and (R) ; Cyclin D1, (F) and (R) ; Cyclin E,: (F) and (R) ; E2F3, (F) and (R) ; C/EBPβ, (F) and (R) .…”

“…After 40 cycles, the specificity of the PCR reaction was confirmed through melting curve analysis and gene expression was quantified by comparing CT values with those of standard 36B4 gene. Most of primer sequences used in the current experiments had been used in our previous study [24]. The sequences of the remaining primers are as follows: CDK2, (F) 5'-CCC TTC CCA AAG CCC TTT TC-3' and (R) 5'-GAA GAG GGG AAG CTG GT-3'; CDK4, (F) 5'-ATG GCT GCC ACT CGA TAT GAA-3' and (R) 5'-TCC ATT AGG AAC TCT CAC AC-3'; Cyclin D1, (F) 5'-GCG TAC CCT GAC ACC AAT CTC-3'and (R) 5'-CTC CTC TTC GCA CTT CTG CTC-3'; Cyclin E,: (F) 5'-GTG GCT CCG ACC TTT CAG TC-3' and (R) 5'-CAC AGT CTT GTC AAT CTT GGC A-3'; E2F3, (F) 5'-CGG TCT GCT CAC CAA GAA GT-3' and (R) 5'-CCT CTT CTG CAC CTT GAG CA-3'; C/EBPβ, (F) 5'-CAA CCT GGA GAC GCA GCA CAA G-3' and (R) 5'-GCT TGA ACA AGT TCG GCA GGG T-3'.…”

SLC3A2 and SLC7A2 Mediate the Exogenous Putrescine-Induced Adipocyte Differentiation

“…3T3-L1 preadipocytes were maintained in Dulbecco’s modified Essential medium (DMEM; Welgene, Korea) supplemented with 10% bovine calf serum (Welgene), 100 U/ml penicillin, and 100 μg/ml streptomycin, at 37℃ with 10% CO 2 . The differentiation of 3T3-L1 cells was induced by an induction medium containing 0.2 × MDI in order to examine the stimulating effect of the polyamine, as described previously ( Yeon et al, 2021 ). In particular, in order to induce differentiation, 3T3-L1 preadipocytes were seeded in a 12-well culture plate at a density of 5.0 × 10 4 cells per well.…”

“…36B4 was used as an internal control in order to calculate the expression levels. The sequences of the primers used in the experiment are the same as in a previous study ( Yeon et al, 2021 ).…”