Methanol Extract of Unfermented Theobroma Cacao Promotes Normal Lipid Profile of Wistar Rats

Abstract

Abnormal lipid metabolism is a major pathogenic factor for various cardiovascular diseases. The present study investigated the lipid profile of methanol extract of unfermented Theobroma cacao (TC) in Wistar rats. 24 Male Wistar rats were divided equally into four groups. Group 1 was the control group, and was administered 0.9% normal saline. Groups 2, 3 and 4 were administered 200mg/kg, 400mg/kg and 800mg/kg methanol extract of unfermented TC. Administration was via oral gavage and lasted for 21 days. The rats were sacrificed under chloroform anaesthesia. Blood was collected through cardiac puncture, allowed to clot, and later centrifuged to get serum. Laboratory assays were done for serum concentrations of total cholesterol (Tc), triacylglycerides (TAG), high density lipoprotein (HDL-c), and low density lipoprotein (LDL-c). Administration of TC extract resulted in an increase (p<0.05) serum concentration of HDL-c, with a consequent reduction in the serum concentrations of Tc, TAG, and LDL-c when compared with the control. The observed results showed that consumption of unfermented Theobroma cacao within the experimental dose promotes normal lipid profile of Wistar rats. Thus, if these results are extrapolated to man, consumption of unfermented Theobroma cacao is encouraged.

Dietary Theobroma cocao Prevents Hepatotoxicity Secondary to Myocardial Injury

“…One such plant with high antioxidant properties is Theobroma cacao [17]. Theobroma cacao is reported to suppress the development of atherosclerotic lesions [18], decrease platelet hyperactivity [19], increase dermal blood flow [20], decrease oxidation of LDL cholesterol and promote normal lipid profile [21]. It also inhibits the proliferation of human breast cancer cells [22].…”