Methanolic Crude Extract of Hagenia abyssinica Possesses Significant Antidiarrheal Effect: Evidence for In Vivo Antidiarrheal Activity

Kifle, Zemene Demelash; Atnafie, Seyfe Asrade; Tadesse, Takele; Belachew, Teshome Fentik

doi:10.1155/2021/9944629

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…This model was done according to the method described earlier by Kifle et al [ 44 ]. Swiss albino mice of either sex were fasted for 18 h from food with free access to water and were randomly allocated to five groups with six mice per group.…”

Section: Determination Of Antidiarrheal Activitymentioning

confidence: 99%

Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice

Andargie

Sisay

Alemu

2022

Evidence-Based Complementary and Alternative Medicine

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Background. Withaniasomnifera is an important medicinal plant for the treatment of diarrhea in Ethiopian folklore medicine. The aim of this study was to evaluate the antidiarrheal activity of Withania somnifera leaves in Swiss albino mice. Materials and Methods. Hydromethanolic crude extraction and solvent fractionation were done using cold maceration technique. 80% methanol was used as a solvent in crude extraction, while distilled water, n-butanol, and chloroform were employed during fractionation. Castor oil-induced diarrhea, enteropooling, and gastrointestinal motility models were employed to evaluate antidiarrheal activity. Mice were randomly divided into five groups (six mice per group): negative control, which received 2% Tween 80 in distilled water; positive control, which received 3 mg/kg loperamide; and three test groups (III, IV, and V), which were treated with 100 mg/kg, 200 mg/kg, and 400 mg/kg of crude extract and solvent fractions, respectively. Results. The crude extract, aqueous, and n-butanol fractions significantly delayed the onset of diarrhea at 200 mg/kg and 400 mg/kg dose. There was a significant reduction in the number and weight of stools at all tested doses of the crude extract and aqueous fraction, and at 200 mg/kg and 400 mg/kg of n-butanol fraction. Significant reduction in volume and weight of intestinal contents was observed at all tested doses of the crude extract, and at 200 mg/kg and 400 mg/kg of aqueous and n-butanol fractions. All tested doses of the crude extract and 200 mg/kg and 400 mg/kg of the aqueous and n-butanol fractions significantly reduced the motility of charcoal meal. Conclusion. This study demonstrated that the crude extract and solvent fractions of the Withania somnifera leaves have antidiarrheal activity and supported the folklore use of the plant.

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Section: Determination Of Antidiarrheal Activitymentioning

confidence: 99%

Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice

Andargie

Sisay

Alemu

2022

Evidence-Based Complementary and Alternative Medicine

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“…Embelia ribes possess significant potential in the prevention and treatment of several chronic diseases, including arthritis, bacterial infections, cancer, cardiovascular diseases, diabetes, neurological problems, and wound healing [ 64 ]. Hagenia abyssinica (Rosaceae) is one of the most-used medicinal plants for the treatment of diarrhea and to treat diabetes mellitus in some regions of Africa [ 65 , 66 ]. Post-hatched chicks orally received treatment either with a high dose of 0.25 g (5 g/kg per day) or a low dose of 0.025 g (0.5 g/kg per day) of Embelia ribes for 1 or 5 days or Hagenia abyssinica for 1 or 9 days.…”

Section: Natural Productsmentioning

confidence: 99%

Retinal Toxicity Induced by Chemical Agents

Araújo

Brito

Pereira-Figueiredo

et al. 2022

IJMS

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Vision is an important sense for humans, and visual impairment/blindness has a huge impact in daily life. The retina is a nervous tissue that is essential for visual processing since it possesses light sensors (photoreceptors) and performs a pre-processing of visual information. Thus, retinal cell dysfunction or degeneration affects visual ability and several general aspects of the day-to-day of a person’s lives. The retina has a blood–retinal barrier, which protects the tissue from a wide range of molecules or microorganisms. However, several agents, coming from systemic pathways, reach the retina and influence its function and survival. Pesticides are still used worldwide for agriculture, contaminating food with substances that could reach the retina. Natural products have also been used for therapeutic purposes and are another group of substances that can get to the retina. Finally, a wide number of medicines administered for different diseases can also affect the retina. The present review aimed to gather recent information about the hazard of these products to the retina, which could be used to encourage the search for more healthy, suitable, or less risky agents.

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“…Some models, including Castor Oil-Induced Diarrhea, Castro Oil-Induced Gastrointestinal Motility, and Castor Oil-Induced Enteropooling Activity, were used to evaluate the in vivo antidiarrheal effects of dragon fruit peel methanol extract. The in vivo study used 25 male Wistar rats (2-3 months,150-200 g) that acclimatized for one week and rats have been selected accordingly, then received some treatments as described in Table I (Abdela, 2019;Kifle, Atnafie, et al, 2021;Kifle, Kidanu, et al, 2021).…”

Section: In Vivo Antidiarrheal Assaymentioning

confidence: 99%

In Vitro and In Vivo Antidiarrheal Activity of Dragon Fruit Peels Methanolic Extract

Yosefa,

Tandanu,

Leslie

et al. 2023

Trad.Med.J

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Diarrhea is a global major health problem reported by 2.5 billion cases annually. Diarrhea accounts for 9% of child death worldwide or the second leading cause of death among toddlers. Many local people have used traditional medicine for diarrhea therapy. Dragon fruit is a herbal plant with high vitamins and various nutritious compounds. Therefore, this study determined antidiarrheal activity from dragon fruit peel methanolic extract (Hylocereus polyrhizus). This study was experimental with in vitro and in vivo models. The in vitro model used disc diffusion and microdilution assay against the diarrhea-causing pathogen (Escherichia coli). Meanwhile, the in vivo model used male Wistar rats induced by Castro oil. This study showed that the minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) of dragon fruit peel extract against Escherichia coli were 50 and 100 mg/ml, respectively. The increased dose of dragon fruit peel methanol extract has increased the antidiarrheal index. The highest and lowest antidiarrheal index was found in the dragon fruit methanol extract-3 (76.56%) and dragon fruit methanol extract-1 (43.28%). The dragon fruit peel methanol extract can be proposed as antidiarrhea and antibacterial therapy against the diarrhea-causing pathogen.

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Methanolic Crude Extract of Hagenia abyssinica Possesses Significant Antidiarrheal Effect: Evidence for In Vivo Antidiarrheal Activity

Cited by 6 publications

References 42 publications

Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice

Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice

Retinal Toxicity Induced by Chemical Agents

In Vitro and In Vivo Antidiarrheal Activity of Dragon Fruit Peels Methanolic Extract

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