Methanolic extract of Crotalaria longirostrata: Identification of secondary metabolites and insecticidal effect

Abstract

The complications caused by the generation of resistance in the psyllid Bactericera cockerelli by chemical pesticides, raise the need for the alternative use of products that reduce resistance and ensure better control. The species Crotalaria longirostrata is a source of secondary metabolites, which show possible activity against insect pests. The objective of the research was to identify the compounds of the crude methanolic extract of C. longirostrata leaves, as well as to evaluate their biological activity on B. cockerelli nymphs. Twenty-five compounds were identified by Gas Chromatography coupled to Mass Spectrometry (GC-MS) analysis; the most abundant was 1β,2β-epoxy-1α-methoxymethyl-8α-pyrrolizidine; an alkaloid of the iminosugar group, reported for the first time in C. longirostrata, of which concentrations from 2 to 30 mg/mL of the methanolic extract had to be prepared to evaluate on B. cockerelli nymphs. It showed that at 48 h, there was a mortality of 73.2%-100% in the treatments of 8, 12, 16, 20, and 30 mg/mL. The mean lethal concentration (LC50) was 4.78 mg/mL and LC95 14,52 mg/mL. The results obtained with the methanolic extract of C. longirostrata leaves for controlling the insect B. cockerelli suggest that it can potentially be used as an alternative insecticide of botanical origin to manage the insect.

Phytotoxicity of Extracts of Argemone mexicana and Crotalaria longirostrata on Tomato Seedling Physiology

“…Aqueous extracts of Posidonia oceanica revealed that phenolic compounds, glycosides, and terpenes played a crucial role in the development of the roots and leaves of S. lycopersicum and Cucumis sativus [22]. The methanolic extracts of C. longirostrata and A. mexicana used in this study contain secondary metabolites previously identified as significant components in these plants [23,24].…”

Toxicological and Sedative Effects of Chipilin (Crotalaria longirostrata) Leaf Extracts Obtained by Maceration and Supercritical Fluid Extraction

“…PAs from plants are toxic compounds that can cause mutations in DNA in herbivores and humans . The identification of one alkaloid belonging to the iminosugar group was reported and related to Chipilin’s insecticidal action . The oxaspiro compound, reported as a diketone or akyl-ketone, has been detected by GC-MS in Brasiliorchis schunkeana and Ficus religiosa seeds. , Several activities such as steroidal antimineralocorticoid, antiandrogen, and glucocorticoid have been reported for this compound .…”

“…As a folk medicine, Chipilin extracts are used to treat several health conditions. ,, The antimicrobial, antifungal, insecticidal, and anticancer activities of acetonic and methanolic extracts of Chipilin leaves and branches have been reported. − As an ethnomedicine, Chipilin is recommended as an evening meal to treat insomnia and anxiety. ,− However, its pharmacological activity as a sedative agent is not well-known; in addition, little is known about the compounds that are present in Chipilin extracts that could be related to this action. Thus, further research is needed. , On the other hand, the selection of an efficient extraction method is decisive for the identification of novel phytochemicals.…”

Antagonistic Activity of Endophytic Fungi Isolated From Aloe Vera Leaves Against Some Plant Pathogenic Fungi

“…Hexanedioic acid, bis(2ethylhexyl) ester is one of the compounds illustrated by the GC-MS assay of the alcoholic extract of Lasiodiplodia pseudotheobromae, the extract showed antibacterial activities against both Grampositive and Gram-negative bacteria (23). The compound -Pyrimidine, 4,6-dimethoxy-5-nitro was among the compounds detected by GC-MS examination of the methanolic extract of Crotalaria longirostrata leaves, the extract showed activity against the nymph insect pest Bactericera cockerelli (31). Cycloheptasiloxane, tetracamethyl-showed antifungal activities (38) as well as antioxidant, antimicrobial and cytotoxic properties against a range of microbes (24).…”