Methanolic extract of Ephedra ciliata promotes wound healing and arrests inflammatory cascade in vivo through downregulation of TNF-α

Yaseen, Hafiza Sidra; Asif, Muhammad; Saadullah, Malik; Mahrukh, .; Asghar, Sajid; Shams, Muhammad Usman; Bazmi, Rizwan Rashid; Saleem, Mohammad; Yousaf, Hafiz Muhammad; Yaseen, Maria

doi:10.1007/s10787-020-00713-7

Cited by 30 publications

(19 citation statements)

References 34 publications

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“…Contrarily, in the murine hindlimb ischemia model, quercetin glucosides enhanced recovery of blood flow to the ischemic leg, manifested by increased capillary density, suggesting its ability to promote angiogenesis in ischemic tissues [37]. Likewise, Q elicited improvement in vessel formation in vivo [38]. In vitro, a relatively lower concentration of Q (20 μM), as used in the current study, increased HUVEC survival and migration after exposure to high glucose by decreasing oxidative stress [39].…”

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confidence: 60%

Quercetin Reverses Cardiac Systolic Dysfunction in Mice Fed with a High‐Fat Diet: Role of Angiogenesis

Kim

Jiang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Global consumption of high-fat diets (HFD) is associated with an increased incidence of cardiometabolic syndrome and cardiac injury, warranting identification of cardioprotective strategies. Cardioprotective effects of quercetin (Q) have mostly been evaluated in ischemic heart disease models and attributed to senolysis. We hypothesized that Q could alleviate murine cardiac damage caused by HFD by restoring the myocardial microcirculation. C57BL/6J mice were fed standard chow or HFD for 6 months and then treated with Q (50 mg/kg) or vehicle 5-day biweekly for 10 additional weeks. Left ventricular (LV) cardiac function was studied in vivo using magnetic resonance imaging, and intramyocardial fat deposition, microvascular density, oxidative stress, and senescence were analyzed ex vivo. Additionally, direct angiogenic effects of Q were studied in vitro in HUVECs. HFD increased body weight, heart weight, total cholesterol, and triglyceride levels, whereas Q normalized heart weight and triglycerides. LV ejection fraction was lower in HFD vs. control mice ( 56.20 ± 15.8 % vs. 73.38 ± 5.04 % , respectively, P < 0.05 ), but improved in HFD + Q mice ( 67.42 ± 7.50 % , P < 0.05 , vs. HFD). Q also prevented cardiac fat accumulation and reduced HFD-induced cardiac fibrosis, cardiomyocyte hypertrophy, oxidative stress, and vascular rarefaction. Cardiac senescence was not observed in any group. In vitro, ox-LDL reduced HUVEC tube formation activity, which Q effectively improved. Quercetin may directly induce angiogenesis and decrease myocardial oxidative stress, which might account for its cardioprotective effects in the murine HFD-fed murine heart independently from senolytic activity. Furthermore, its beneficial effects might be partly attributed to a decrease in plasma triglycerides and intramyocardial fat deposition.

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confidence: 60%

Quercetin Reverses Cardiac Systolic Dysfunction in Mice Fed with a High‐Fat Diet: Role of Angiogenesis

Kim

Jiang

et al. 2021

Oxidative Medicine and Cellular Longevity

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“… 30 Excessive production of ROS at wound sites produces toxic effects, causing inflammation and the degradation of repair mechanisms. 31 …”

Section: Discussionmentioning

confidence: 99%

“…These cells also release TNF-α and other pro-inflammatory cytokines, which are, in turn, responsible for chronic inflammatory responses. 31 …”

Section: Discussionmentioning

confidence: 99%

Growth factor stimulation for the healing of traumatic ulcers with liquid rice hull smoke

Arundina

Diyatri

Surboyo

et al. 2021

Journal of Taibah University Medical Sciences

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Objective The healing process of a traumatic ulcer requires growth factors to rebuild the lost tissue after the inflammatory process has been completed. Liquid rice hull smoke (LR-HS) has shown unique anti-inflammatory properties. This study analyses the role of LR-HS in growth factor stimulation for the healing of traumatic ulcers, such as fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and collagen type 1 (COL-1) expression. Methods We obtained LR-HS from the pyrolysis of rice hulls. Traumatic ulcers were created in the labial fornix incisive inferior of Wistar rats and treated with LR-HS once a day for 3, 5, and 7 days. The control group was treated with sterile water. Each animal was sacrificed after treatment, and its labial fornix incisive inferior tissues were biopsied and immunohistochemically stained to examine FGF, VEGF, PDGF, and COL-1 expression. Result The treatment of traumatic ulcers with LR-HS showed an increase in FGF, VEGF, PDGF, and COL-1 expression. VEGF expression increased under LR-HS treatment compared with the control 7-day treatment groups ( p < 0.000). FGF and COL-1 expression increased under LR-HS treatment compared with the control 5- and 7-day treatment groups ( p < 0.000). PDGF expression increased after treatment with LR-HS for 3, 5, and 7 days ( p < 0.000). Conclusion This study has demonstrated that LR-HS can induce the expression of growth factors during the healing of a traumatic ulcer using immunohistochemical staining. We suggest that LR-HS can be used as a herbal medicine for oral ulcer therapy.

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“…Many researches have indicated that several key cellular factors are involved in the process of burn repair, such as tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), and transforming growth factor-beta1 (TGF-β1) (Kim et al, 2020;Yaseen et al, 2020). If the body acquires an infection when the skin is damaged, it is stimulated to secrete the proinflammatory cytokines, such as TNF-α.…”

Section: Introductionmentioning

confidence: 99%

Burn Ointment Promotes Cutaneous Wound Healing by Modulating the PI3K/AKT/mTOR Signaling Pathway

Gan

et al. 2021

Front. Pharmacol.

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Burn ointment (BO) is a clinically useful medicine for the treatment of burns and scalds. However, there is no enough scientific evidence to report the effect of BO on wound healing and its analgesic and anti-inflammatory efficacy. The aim of this work was to evaluate the anti-inflammatory and analgesic efficacy of BO and to reveal the potential wound healing properties and related mechanisms of BO. In this work, the content of active ingredients of BO was determined by high-performance liquid chromatography (HPLC). Two animal models of inflammation were used to study its anti-inflammatory activity, and a hot plate method was used to evaluate its analgesic effect. In addition, mouse incision and rat burn models were used to investigate the effect of BO on the anti-inflammatory and wound healing mechanisms. The results showed that BO was safe for topical application, and BO could significantly inhibit auricular swelling in mice and paw swelling in rats and significantly prolong the latency period of paw licking in the hot plate experiment in mice. It can also accelerate wound healing and repair scars by promoting the formation of new epithelial tissues in rat burn models. In addition, BO significantly downregulated the serum level of TNF-α and significantly increased the serum levels of VEGF and TGF-β1. Also, BO promoted the expression of collagen I and increased the ratio in p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR pathways. Our results demonstrate the safety and efficacy of BO and suggest that activation of the PI3K/AKT/mTOR signaling pathway may play an important role in the promotion of wound healing by BO.

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Methanolic extract of Ephedra ciliata promotes wound healing and arrests inflammatory cascade in vivo through downregulation of TNF-α

Cited by 30 publications

References 34 publications

Quercetin Reverses Cardiac Systolic Dysfunction in Mice Fed with a High‐Fat Diet: Role of Angiogenesis

Quercetin Reverses Cardiac Systolic Dysfunction in Mice Fed with a High‐Fat Diet: Role of Angiogenesis

Growth factor stimulation for the healing of traumatic ulcers with liquid rice hull smoke

Burn Ointment Promotes Cutaneous Wound Healing by Modulating the PI3K/AKT/mTOR Signaling Pathway

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