Methanolic Extract ofCuminum cyminumInhibits Ovariectomy-Induced Bone Loss in Rats

Abstract

Several animal and clinical studies have shown that phytoestrogens, plant-derived estrogenic compounds, can be useful in treating postmenopausal osteoporosis. Phytoestrogens and phytoestrogen-containing plants are currently under active investigation for their role in estrogen-related disorders. The present study deals with anti-osteoporotic evaluation of phytoestrogen-rich plant Cuminum cyminum, commonly known as cumin. Adult Sprague-Dawley rats were bilaterally ovariectomized (OVX) and randomly assigned to 3 groups (10 rats/group). Additional 10 animals were sham operated. OVX and sham control groups were orally administered with vehicle while the other two OVX groups were administered 0.15 mg/kg estradiol and 1 g/kg of methanolic extract of Cuminum cyminum fruits (MCC) in two divided doses for 10 weeks. At the end of the study blood, bones and uteri of the animals were collected. Serum was evaluated for calcium, phosphorus, alkaline phosphatase and tartarate resistant acid phosphatase. Bone density, ash density, mineral content and mechanical strength of bones were evaluated. Scanning electron microscopic (SEM) analysis of bones (tibia) was performed. Results were analyzed using ANOVA and Tukeys multiple comparison test. MCC (1 g/kg, p.o.) significantly reduced urinary calcium excretion and significantly increased calcium content and mechanical strength of bones in comparison to OVX control. It showed greater bone and ash densities and improved microarchitecture of bones in SEM analysis. Unlike estradiol it did not affect body weight gain and weight of atrophic uterus in OVX animals. MCC prevented ovariectomy-induced bone loss in rats with no anabolic effect on atrophic uterus. The osteoprotective effect was comparable with estradiol.

Identification and Qualitative Analysis of β-Sitosterol and Some Phytoestrogens in In Vivo and In-Vitro Samples of Lepidium sativum: A Semi-Arid Bone Healing Plant

“…HPTLC technique with other biochemical assays supplemented evidence to confirm the presence of some bioactive compounds, which have provided the plants an important position in folkloric usage [15] In the present study, quantitative and qualitative biochemical methods can be employed as a stability indicator [16] that can be used for the routine quality control analysis and quantitative determination of β-sitosterol from Lepidium sativum. Results obtained by HPTLC for diazdzein and formononetin were coincided with the results of [17][18][19]. Although originally identified in cementum, PTPLa/ CAP is very effective at inducing bone repair and healing and therefore this novel molecule has a great potential to be used for mineralized tissue bioengineering and tissue regeneration studied by [20].…”

Organ Toxicity and Estrogen Like Effects of Cuminum Cyminum.L Seed Essential Oil: A Hormonal, Histopathological and Immunohistochemical Study in Female Mice

“…cyminum essential oil at dose levels of 250,500 and 1000 mg/ day was orally administered to female mice for 14 days caused marked elevation in the body weight , uterus and estradiol levels(p<0.001) whereas the progesterone levels were decreased significantly in all dose groups including 25 mg/kg after 28 days study (p=0.001) . Phytoestrogen properties of cumin extract has been previously described (25)and it was identified useful in treating postmenopausal osteoporosis and estrogen-related disorders in bilaterally ovariectomized rat as an osteoprotective product comparable with estradiol (26).This study suggests the phyotoestrogenic effects of cumin essential oil which should be confirmed by later studies using specific models. On the other side, another study showed a marked reduction in sperm density in the cauda epididymis and testes and sperm motility in the cauda epididymis as well as fertility reduction, significant decreases in the number of testicular cells, secondary spermatocytes and round spermatids (27) with its endocrine disruptive effects.…”

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

“…Basu et al (2001) revealed that the osteoporosis could be eliminated when oxidative stress is prevented. Unfortunately, the current pharmacological therapy used for treatment of osteoporosis have serious adverse effects besides its inability to restore lost bone mass (Shirke et al, 2008). Bisphosphonates administration is widely used for management of osteoporosis.…”