Methanolic extract of Ruta graveolens L. inhibits inflammation and oxidative stress in adjuvant induced model of arthritis in rats

Ratheesh, M.; Shyni, G. L.; Helen, A.

doi:10.1007/s10787-009-8044-0

Cited by 44 publications

(25 citation statements)

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“…In inflamed tissue, COX -2 is unregulated. This may be responsible for the enhanced production of prostaglandins (Ratheesh et al, 2009). In a study conducted by Ratheesh et al (2009), the reduction of rat paw oedema and the reduced activity of COX -2 indicated anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

“…This may be responsible for the enhanced production of prostaglandins (Ratheesh et al, 2009). In a study conducted by Ratheesh et al (2009), the reduction of rat paw oedema and the reduced activity of COX -2 indicated anti-inflammatory effects. Amabeoku et al (2012), reported that C. orbiculata reduced carrageenan-induced rat paw oedema, and suggested that the plant may have affected inflammatory mediators to produce its anti-inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

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Antinociceptive, Anti-Inflammatory and Antipyretic Activities of the Leaf Methanol Extract of Ruta Graveolens L. (Rutaceae) in Mice and Rats

Loonat

Amabeoku

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Ruta graveolens has been used to treat toothache, earache, rheumatism and fever with little scientific evidence corroborating these uses. Materials and Methods:The leaf methanol extract of Ruta graveolens was evaluated for antinociceptive activity using the acetic acid writhing and hot-plate tests in mice, also anti-inflammatory and antipyretic activities using the carrageenan-induced oedema and E. coli-induced pyrexia tests in rats, respectively. Results: R. graveolens (100 mg/kg, i.p.), significantly reduced the number of acetic acid-induced writhes by 54 %. R. graveolens (400 mg/kg, i.p.), significantly delayed the reaction time in mice to thermal stimulation 15, 30, 45, and 60 min after treatment. Combined treatment of the lowest and sub-effective doses of the leaf methanol extract (25 mg/kg, i.p.), and indomethacin (10 mg/kg, i.p.) significantly reduced the number of acetic acidinduced writhes in mice. The leaf methanol extract of R. graveolens (50 -400 mg/kg, i.p.), significantly reduced carrageenan-induced oedema over the 4 h period of testing. Combined treatment of the lowest doses of R. graveolens (25 mg/kg, i.p.) and indomethacin (2 mg/kg, i.p.) produced a significant reduction in carrageenan-induced oedema over the 4 h period of testing. R. graveolens (100 -400 mg/kg, i.p.) significantly reduced E. coliinduced pyrexia over the 5 h period of testing. Given together, the lowest dose of R. graveolens (25 mg/kg, i.p.) and pentoxifylline (10 mg/kg, i.p.) produced a significant reduction in pyrexia induced by E. coli (50 μg/kg, i.m.) over the 5 h period of measurement. The LD 50 value obtained for R. graveolens was greater than 4000mg/kg (p.o), suggesting that the plant species may be safe in or nontoxic to mice. Conclusion:The data obtained indicate that R. graveolens has antinociceptive, anti-inflammatory and antipyretic activities, justifying the use of the plant species by traditional medicine practitioners in the management and treatment of pain, inflammation and fever.Key words: Ruta graveolens, Rutaceae, antinociceptive activity, anti-inflammatory activity, Antipyretic activity, leaf methanol extract IntroductionPain, inflammation and fever are essential events that occur in response to underlying conditions such as toothache and earache, autoimmune diseases and infections. These events have been well managed and treated with the numerous effective orthodox medicines available. However, an estimated 70% of the South African population in both rural and urban areas rely on the use of plants for medicinal purposes (Light et al., 2005;Tabuti et al., 2003;Amabeoku et al., 1998). One of such plants is Ruta graveolens L. Ruta graveolens belongs to the Rutaceae family. Native to Europe and commonly grown in South Africa, it is locally known as Rue or the Herb of Grace in English, and "wynruit" or "binnewortel" in Afrikaans. R. graveolens is a woody, evergreen shrub of up to a metre in height with a characteristic aromatic scent. The plant has yellow flowers that are made up of 4 petals e...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antinociceptive, Anti-Inflammatory and Antipyretic Activities of the Leaf Methanol Extract of Ruta Graveolens L. (Rutaceae) in Mice and Rats

Loonat

Amabeoku

2014

Afr. J. Trad. Compl. Alt. Med.

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“…Food efficiency increased significantly when rats fed the diets containing fenugreek with date extract, primrose oil in combination with date extract or fish oil with date extract. (Ratheesh et al 2009). ESR has been reported to be an excellent index for assessing the therapeutic effect of anti-inflammatory agents (Arvidsoon et al, 1998).…”

Section: Biochemical Parameters Of Arthritic Ratsmentioning

confidence: 99%

Preparation and evaluation of functional foods in adjuvant arthritis

Al‐Okbi¹,

Mohamed²

2012

Grasas y Aceites

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Adjuvant arthritis is an animal model that closely resembles rheumatoid arthritis in humans. It is a successful working model used to study new anti-inflammatory agents. In previous studies (animal and clinical) we have shown that evening primrose oil, fish oil and the methanol extract of date fruits and fenugreek seeds have anti-inflammatory activity and that the methanol extract of dates has an antioxidant effect. Based on these studies, the aim of the present study was to prepare 7 functional foods containing such bioactive fractions separately or in combination and to evaluate them in adjuvant arthritis in rats, study the stability of bioactive ingredients and evaluate their sensory properties. The studied biochemical parameters were erythrocyte sedimentation rate, erythrocyte superoxide dismutase, glutathione peroxidase and plasma copper, zinc and interlukin 2. Nutritional parameters, including body weight gain, food intake and food efficiency ratio were monitored during the feeding of the functional foods. The bioactive ingredients assessed were total phenolic contents and fatty acids. The results showed improvement in the biochemical parameters, body weight gain and food efficiency ratio of arthritic rats fed on the functional foods with different degrees. All the prepared functional foods were sensory accepted. The active ingredients showed stability during storage. In conclusion, all the tested functional foods showed promising antiinflammatory activity and were determined to be acceptable through sensory evaluation which means that their potential beneficial use as dietary supplements in rheumatoid arthritis patients may be recommended. La artritis adyuvante es un modelo utilizado en animales y se caracteriza por ser muy parecida a la artritis reumatoide en humanos. Se trata de un modelo de trabajo utilizado con éxito para estudiar nuevos agentes anti-inflamatorios. En estudios previos (animales y clínica) hemos demostrado que el aceite de onagra, el aceite de pescado y los extractos metanólicos de semillas de fenogreco o alholva y de dátiles tienen actividad anti-inflamatoria y que el extracto metanólico de dátiles tiene efecto antioxidante. Basado en estos estudios, el objetivo del presente trabajo consistió en preparar alimentos funcionales con siete fracciones bioactivas por separado y conjuntamente y evaluarlas en artritis adyuvante en ratas, estudiar la estabilidad de los principios bioactivos y evaluar sus propiedades sensoriales. Los parámetros bioquímicas estudiados fueron la velocidad de sedimentación globular, la enzima superóxido dismutasa eritrocitaria, la glutatión peroxidasa, cobre plasmático, y la zinc interleucina-2. Los parámetros nutricionales, incluyendo ganancia de peso corporal, la ingesta de alimentos y la relación de eficiencia alimentaria se han seguido durante la ingesta de los alimentos funcionales. Los ingredientes bioactivos evaluados fueron el contenido de fenoles totales y ácidos grasos. Los resultados mostraron una mejoría de los parámetros bioquímicos,...

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“…Furanocoumarins were one of the main active constituents of Ruta graveolens and were also reported to be potent antioxidants while total phenolic content showed good correlation with nitric oxide reduction potential. Antioxidant activity of in vitro, culture was significantly higher compared to in vivo plant material [19], [20]. Terminalia chebula were investigated for antilipid peroxidation, antisuperoxide radical formation and free radical scavenging activities [21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

EL-Kamali

Hamed²

2015

Int. J. Sci. World

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In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of Punica granatum showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of Commiphora myrrha 58±0.01 and Azadirachta indica 57±0.20 respectively.

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Methanolic extract of Ruta graveolens L. inhibits inflammation and oxidative stress in adjuvant induced model of arthritis in rats

Cited by 44 publications

References 28 publications

Antinociceptive, Anti-Inflammatory and Antipyretic Activities of the Leaf Methanol Extract of <i>Ruta Graveolens</i> L. (Rutaceae) in Mice and Rats

Antinociceptive, Anti-Inflammatory and Antipyretic Activities of the Leaf Methanol Extract of <i>Ruta Graveolens</i> L. (Rutaceae) in Mice and Rats

Preparation and evaluation of functional foods in adjuvant arthritis

Antioxidant potential of some Sudanese medicinal plants used in traditional medicine

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