Methanolic Extracts from Cultivated Mushrooms Affect the Production of Fumonisins B and Fusaric Acid by Fusarium verticillioides

Abstract

The maize pathogen Fusarium verticillioides and their mycotoxins cause damage to plants, animals, and human health. This work aimed to evaluate the effect of crude extracts (CEs) from Agaricus subrufescens, Lentinula edodes, and Pleurotus ostreatus fruiting bodies on in vitro production of biomass and mycotoxins by two strains of F. verticillioides. Stipes and pilei were separated before extraction for A. subrufescens and L. edodes. Comparative metabolomics and dereplication of phenolic compounds were used to analyze all CEs. Mushroom CEs did not significantly inhibit the production of mycelial biomass at concentrations of 2 mg mL−1. CEs from A. subrufescens (stipes and pilei) and L. edodes pilei inhibited the production of fumonisins B1 + B2 + B3 by 54% to 80%, whereas CE from P. ostreatus had no effect. In contrast, CE from L. edodes stipes dramatically increased the concentration of fumonisins in culture media. Fusaric acid concentration was decreased in cultures by all CEs except L. edodes stipes. Differences in phenolic composition of the extracts may explain the different effects of the CE treatments on the production of mycotoxins. The opposing activities of stipes and pilei from L. edodes offer an opportunity to search for active compounds to control the mycotoxin production by F. verticillioides.

Antifungal and Insecticidal Activities of Selected Plant Species from Cloud Forest of Veracruz, Mexico: A Contribution to the Search of Novel Control Agents against Ambrosia Pest Complexes

“…So, we have proved that our ecological criterium of choosing plants from MCF ecosystems is valid and should be considered for incorporating these plants species in other bioprospecting programs such as drug discovery. In addition, active plant species here described represent opportunities for the isolation and identification of new antifungal compounds against Fusarium spp [36,37] . and bioassay‐guided fractionation approaches should be further considered leading the identification of the bioactive antifungal metabolites.…”

Influence of Sunlight Incidence and Fruit Chemical Features on Oviposition Site Selection in Mango by Anastrepha obliqua: Implications for Management

“…Metabolomic studies based on accurate mass spectrometry represent a powerful tool that has been used to gain insights into more efficient management approaches against notorious plant pests and diseases, such as thrips [80], foraging ants [81] and fusarium wilt [82][83][84]. The untargeted metabolomics study we performed reinforces the idea that A. obliqua females can sense fruit chemical quality, as reported for D. melanogaster [79], since we identified biochemical differences between oviposited and non-oviposited sites in "Criollo" mangos (Figures 4b and 5).…”

Microbial Biocontrol Strategies for Ambrosia Beetles and Their Associated Phytopathogenic Fungi

“…For example, it has been reported that organics extracts from culture filtrates and basidiomes of Agaricus subrufescens (pilei and stipe), Lentinula edodes (pilei), and Pleurotus ostreatus significantly reduced the mycelial growth and production of fusaric acid in Fusarium spp. in vitro (Chen and Huang, 2010;Merel et al, 2020). Interestingly, fusaric acid was recently described as one of the potential virulence factors produced by F. kuroshium (Gutiérrez-Sánchez et al, 2021) and is capable of inducing in vitro foliar damage in avocado, in a similar way to the symptomatology observed in Fusarium dieback.…”