Methanosarcina as the dominant aceticlastic methanogens during mesophilic anaerobic digestion of putrescible waste

Вавилин, В.А.; Qu, Xian; Mazéas, Laurent; Lemunier, M.; Duquennoi, C.; He, Pinjing; Bouchez, Théodore

doi:10.1007/s10482-008-9279-2

Cited by 57 publications

(33 citation statements)

References 31 publications

(30 reference statements)

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“…[22,35,36] These methanogens are acetoclastic methane producers and confirm the importance of this pathway in methanogenesis, as seen during the digestion of SuOC. As a consequence, a direct interaction between bacteria and archaea is envisioned, the main role of the bacterial community during this anaerobic process appeared to be the production of acetate from the polymers constituting the SuOC.…”

Section: Migrationsupporting

confidence: 69%

Feasibility of sunflower oil cake degradation with three different anaerobic consortia

Rincón

Guisado²,

González³

et al. 2011

Journal of Environmental Science and Health, Part A

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Sunflower oil cake (SuOC) is the solid by-product from the sunflower oil extraction process and an important pollutant waste because of its high organic content. For the anaerobic digestion of SuOC three different industrial reactors were compared as inoculum sources. This was done using a biochemical methane production (BMP) test. Inoculum I was a granular biomass from an industrial reactor treating soft-drink wastewaters. Inoculum II was a flocculent biomass from a full-scale reactor treating biosolids generated in an urban wastewater treatment plant. Inoculum III was a granular biomass from an industrial reactor treating brewery wastes. The highest kinetic constant for methane production was achieved using inoculum II. The inoculum sources were analyzed through PCR amplification of 16S rRNA genes and fingerprinting before (t = 0) and after the BMP test (t = 12 days). No significant differences were found in the bacterial community fingerprints between the beginning and the end of the experiments. The bacterial and archaeal communities of inoculum II were further analyzed. The main bacteria found in this inoculum belong to Alphaproteobacteria and Chloroflexi. Of the Archaea detected, Methanomicrobiales and Methanosarcinales made up practically the whole archaeal community. The results showed the importance of selecting an appropriate inoculum in short term processes due to the fact that the major microbial constituents in the initial consortia remained stable throughout anaerobic digestion.

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Section: Migrationsupporting

confidence: 69%

Feasibility of sunflower oil cake degradation with three different anaerobic consortia

Rincón

Guisado²,

González³

et al. 2011

Journal of Environmental Science and Health, Part A

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“…Acetoclastic methanogenesis can produce 13 C-enriched CH 4 that may appear thermogenic in origin (Valentine et al, 2004). Past studies have documented δ 13 C-CH 4 values as high as -30 ‰ for acetoclastic methanogenesis (Penning et al, 2006;Vavilin et al, 2008). In this study, however, some of the δ 13 C-CH 4 values are even more positive than the δ 13 C-CH 4 values for acetoclastic…”

Section: Possible Causes For More Positive δ 13 C-ch 4 Values Found Icontrasting

confidence: 64%

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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Carbon and hydrogen isotope compositions of CH 4 generated via methanogenesis in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation waters grown on coal, acetate and H 2 +CO 2 were investigated. CH 4 production and molecular analysis confirmed the presence of active microbial communities that are able to convert coal into CH 4 using both acetoclastic and hydrogenotrophic pathways. The representative bacterial sequences were dominated by Bacteroidetes, Firmicutes and Deltaproteobacteria, while Methanosaeta and Methanosarcina were the most prevalent archaeal methanogens present in the cultures.CH 4 produced in this study's culturing experiments has δ 13 C values in the range of -50 ‰ to -20 ‰, with most values falling outside the current understanding of the carbon isotopic boundaries for biogenic CH 4 (-110 ‰ to -30 ‰). However, the corresponding apparent carbon isotopic α factor (α c = 1.02±0.006), and isotopic effect (ε c = -20.1 ‰ ±15.3) showed that CH 4 in SSB cultures was predominantly produced by acetoclastic methanogenesis, which is consistent with the results of molecular DNA analysis. In addition, the calculated contribution of CO 2 reduction from the δ 13 C values of coaltreated cultures was overall < 50 %, further confirming the high contribution of the acetoclastic pathway to CH 4 production in the SSB cultures. The outcome of this experimental study also suggests that δ 2 H-CH 4 values may not provide a reliable basis for distinguishing methanogenic pathways, while apparent carbon isotopic fractionation factor (α c ) and isotope effect (ε c ) are considered more useful indicators of the methanogenic pathway.The high δ 13 C-CH 4 values (>-30 ‰) and the dominance of Methanosaeta over Methanosarcina indicate that methanogens within the SSB cultures were operating at low substrate concentrations. An unusually positive δ 13 C-CH 4 suggests a substrate depletion effect, which is thought to be related to a decrease in the relative abundance of key bacterial coal degraders with formation water inoculum storage time. Closer observation of δ 13 C-CH 4 values during the growth of cultures within a single experiment also showed a 13 C-enrichment trend over time. At log phase of growth, the CH 4 produced was 13 C-depleted when compared to the stationary phase that also indicates substrate depletion effects. Finally, the δ 13 C-CH 4 values encountered in this study (as high as -20 ‰) highlight the possible positive extension of δ 13 C-CH 4 values of acetoclastic methanogenesis from those currently reported in the literature for natural and experimental samples (as high as -30 ‰).Keywords: Biogenic gas, coal, culture experiment, isotopic composition INTRODUCTIONArchaeal methanogens are the only known microorganisms capable of forming CH 4 . These strictly anaerobic microbes are restricted to utilizing simple compounds that contain no more than two carbon atoms. As such, complex organic compounds in anoxic environments (e.g., marine sediments, rice paddies, subsurface shales and coalbeds) ...

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“…Acetoclastic methanogenesis can produce 13 C-enriched CH 4 that may appear thermogenic in origin . Past studies have documented δ 13 C-CH 4 values as high as 30 for acetoclastic methanogenesis Petsch et al, 2007;Vavilin et al, 2008). In this study, however, some of the depletion effects or microbial CH 4 oxidation that may result from methanogens that are operating in reverse .…”

Section: Gas Isotopic Composition Of Ssb Culturescontrasting

confidence: 48%

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

Susilawati¹

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Biogenic coal bed methane (CBM) has gained considerable interest because of its potential to be regenerated. The research reported in this thesis evaluated the potential to develop Indonesian coals as CH 4 bioreactors using culture enrichment, molecular phylogenetic and isotopic composition analysis methods. The results increase the current understanding on microbial methanogenesis in coal, with a specific focus on Indonesian coals.Indonesian coals range in rank from lignite to bituminous, with anthracites occurring in specific areas. The coals are low in ash yield and dominated by vitrinite group macerals, with abundant liptinite group macerals, which is thought to make them good targets for methanogenesis.Stable isotope analysis of gas from pilot production wells confirmed the hypothesis that Indonesian CBM is partly biogenic in origin. Culture enrichment studies of formation water from these CBM pilot wells also indicated the presence of active coal to CH 4 consortia that has the capability for degrading native South Sumatra Basin (SSB) coals and foreign Surat Basin coal from Australia. Among the Indonesian coal basins, the SSB currently has the greatest CBM potential and good accessibility for gas, water, and coal sampling that is required to assess the biogenic CH 4 potential of Indonesian coal. Therefore, SSB was targeted for the experiments conducted in this thesis. The investigation of the microbial methanogen community structure grown on different coal substrates showed temporal changes in community structure over time, and suggested some influence of coal substrate on the microbial community composition. The community structure exhibited greater similarity when grown on coal from the same seam but of different ranks (Mangus iii sub-bituminous (SB) Rv 0.5% and Mangus Anthracite (A) Rv 2.2%) than for coal of similar types (Mangus SB Rv 0.5% and Burung SB Rv 0.39%). The obligate acetoclastic Methanosaeta members favor Burung SB coal, while metabolically versatile Methanosarcina members favor the Mangus coals and the obligate hydrogenotrophic methanogens are significant in the control cultures without coal. Regardless of the community structure similarities across all coal cultures, more CH 4 was generated from the lower rank sub-bituminous coal cultures relative to the one high-rank semi anthracite coal. These results suggest a potential relationship between coal type and rank, microbial community composition and CH 4 production, which warrants further investigation.While CH 4 measurements and molecular phylogenetic analysis confirmed the production of biogenic CH 4 in the cultures, the gas sampled from the culture headspace had δ 13 C-CH 4 values ( 52.2‰ to 22.6‰) that mostly fell outside the range currently considered to indicate a biogenic origin. In this study, the apparent carbon fractionation factor (α c =1.02±0.006) and isotope effect (ε c = 20.1‰±15.3) were found to be more useful indicators of methanogenic pathways than the absolute δ 13 C-CH 4 values. Both values agreed with the calculat...

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Methanosarcina as the dominant aceticlastic methanogens during mesophilic anaerobic digestion of putrescible waste

Cited by 57 publications

References 31 publications

Feasibility of sunflower oil cake degradation with three different anaerobic consortia

Feasibility of sunflower oil cake degradation with three different anaerobic consortia

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

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