Methanotrophs Contribute to Nitrogen Fixation in Emergent Macrophytes

Cui, Jiahe; Zhang, Meng; Chen, Linxia; Zhang, Shaohua; Luo, Ying; Cao, Weiwei; Zhao, Ji; Wang, Lixin; Jia, Zhongjun; Bao, Zhihua

doi:10.3389/fmicb.2022.851424

Cited by 14 publications

(7 citation statements)

References 54 publications

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“…The 15 N and 13 C abundances in the freeze-dried soil were measured by a stable-isotope ratio mass spectrometer (Isoprime-100, Elementar). The BNF rates were calculated using the formula (SW × TN/100 × ( 15 N c1 – 15 N c2 )/ 15 N g × 100/MW/ t ) as previously described [ 17 ], where SW is the dried soil weight (1.00 g/reactor), TN is the average nitrogen content (%, w/w), and MW is the molecular weight (30) of 15 N 2 . The 15 N c1 and 15 N c2 represent the respective final and initial 15 N concentrations (atom% excess) in the soils, respectively.…”

Section: Methodsmentioning

confidence: 99%

Ferrihydrite-mediated methanotrophic nitrogen fixation in paddy soil under hypoxia

Yu,

Jia,

Liu

et al. 2024

ISME Communications

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Biological nitrogen fixation (BNF) by methanotrophic bacteria has been shown to play an important role in maintaining fertility. However, this process is still limited to aerobic methane oxidation (AMO) with sufficient oxygen. It has remained unknown whether and how methanotrophic BNF proceeds in hypoxic environments. Herein, we incubated paddy soils with a ferrihydrite-containing mineral salt medium to enrich methanotrophic bacteria in the presence of methane (20%, v/v) under oxygen constraints (0.27%, v/v). The resulting microcosms showed that ferrihydrite-dependent AMO significantly contributed (81%) to total BNF, increasing the 15N fixation rate by 13-fold from 0.02 to 0.28 μmol 15N2 (g dry weight soil) -1 d−1. BNF was reduced by 97% when ferrihydrite was omitted, demonstrating the involvement of ferrihydrite in methanotrophic BNF. DNA stable-isotope probing indicated that Methylocystis, Methylophilaceae and Methylomicrobium were the dominant methanotrophs/methylotrophs that assimilated labelled isotopes (13C or 15N) into biomass. Metagenomic binning combined with electrochemical analysis suggested that Methylocystis and Methylophilaceae had the potential to perform methane-induced BNF and likely utilized riboflavin and c-type cytochromes as electron carriers for ferrihydrite reduction. It was concluded that ferrihydrite mediated methanotrophic BNF by methanotrophs/methylotrophs solely or in conjunction with iron-reducing bacteria. Overall, this study revealed a previously overlooked yet pronounced coupling of iron-dependent AMO to BNF and improves our understanding of methanotrophic BNF in hypoxic zones.

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Section: Methodsmentioning

confidence: 99%

Ferrihydrite-mediated methanotrophic nitrogen fixation in paddy soil under hypoxia

Yu,

Jia,

Liu

et al. 2024

ISME Communications

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“…Another study also revealed that methanotroph inoculation on rice fields lowered CH 4 emissions by 60% and augmented rice yield by 35% [26]. The augmentation of rice yields, known as several methanotrophs, can increase the soil N availability, enhancing photosynthate allocation to grains [26,31,32]. However, these studies examined differences in chemical fertilizer dosages or types of methanotrophs.…”

Section: Effects Of Methanotroph-inoculated Bde On Methane and Nitrou...mentioning

confidence: 99%

“…Another study also revealed that methanotroph inoculation on rice fields reduced CH 4 emissions by 60% and increased the rice yield by 35% [26]. The augmentation of rice yields, known as several methanotrophs, can increase the soil N availability, enhancing photosynthate allocation to grains [26,31,32]. However, the studies above examined the potential of CH 4 emission reductions by inoculating methanotrophs on rice fields under the difference of fertilizer application dosages or fertilizer types, while fields amended with external organic carbon or higher dissolved organic carbon remains uncertain with methanotroph inoculation.…”

Section: Introductionmentioning

confidence: 98%

“…These strains are affiliated with type II methanotrophs, which adapt to a fluctuating environment and high CH 4 concentration, indicating the potential for reducing GHG emission based on methanotroph inoculation in rice fields of the VMD. Although the efficacy of methanotroph inoculation in reducing GHG emissions and promoting grain yield has been observed by several strains in previous studies [26,31,32], the CH 4 oxidation potential of methanotrophic strains isolated in the VMD's rice fields has not been examined. In addition, the utilization of BDE for rice fields has recently been popularized in the VMD, particularly in small-scale livestock farming.…”

Section: Introductionmentioning

confidence: 99%

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Methanotrophic Inoculation Reduces Methane Emissions from Rice Cultivation Supplied with Pig-Livestock Biogas Digestive Effluent

Thao,

Tarao,

Takada

et al. 2024

Agronomy

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Biogas digestive effluent (BDE) is a nutrient-enriched source that can be utilized as an organic fertilizer for rice cultivation without synthetic fertilizer (SF) application. However, a primary concern is the stimulation of methane (CH4) emissions due to the enrichment of the labile organic carbon, a favorite substrate of methanogenic archaea. Methanotrophs potentially reduce greenhouse gas (GHG) emissions from rice fields owing to metabolizing CH4 as a carbon source and energy. We therefore examined the effect of the application of methanotroph-inoculated BDE to the rice cultivated paddy soil on GHG emissions and rice productivity under a pot experiment. Methanotrophs (Methylosinus sp. and Methylocystis sp.), isolated from the Vietnamese Mekong Delta’s rice fields, were separately inoculated to the heated BDE, followed by a 5-day preincubation. Methanotroph-inoculated BDE was supplied to rice cultivation to substitute SF at 50% or 100% in terms of nitrogen amount. The results showed that the total CH4 emissions increased ~34% with the application of BDE. CH4 emissions were significantly reduced by ~17–21% and ~28–44% under the application of methanotroph-inoculated BDE at 100% and 50%, respectively. The reduction in CH4 was commensurate with the augmentation of pmoA transcript copy number under methanotroph-inoculated BDE. In addition, methanotroph-inoculated BDE application did not increase nitrous oxide (N2O) emissions and adversely affect rice growth and grain productivity. This study highlighted the BDE-recirculated feasibility for a lower CH4 emission rice production based on methanotrophs where high CH4-emitting fields were confirmed.

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“…Studies have shown that soil methanotrophs contribute 10–20% to global methane consumption, playing a key role in methane balance [ 20 ]. The microhabitat of plants’ rhizosphere is closely linked to the methane oxidation process in soil, as methanotrophs are usually concentrated in the rhizosphere [ 21 , 22 ]. Oxygen is diffused from the roots into the plants’ rhizosphere and the adjacent soil through radial oxygen loss, creating a favorable microhabitat for aerobic methane oxidation [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Selenium-Induced Enhancement in Growth and Rhizosphere Soil Methane Oxidation of Prickly Pear

Wang,

Xie,

Chen

et al. 2024

Plants

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As an essential element for plants, animals, and humans, selenium (Se) has been shown to participate in microbial methane oxidation. We studied the growth response and rhizosphere methane oxidation of an economic crop (prickly pear, Rosa roxburghii Tratt) through three treatments (Se0.6 mg/kg, Se2.0 mg/kg, and Se10 mg/kg) and a control (Se0 mg/kg) in a two-month pot experiment. The results showed that the height, total biomass, root biomass, and leaf biomass of prickly pear were significantly increased in the Se0.6 and Se2.0 treatments. The root-to-shoot ratio of prickly pear reached a maximum value in the Se2 treatment. The leaf carotenoid contents significantly increased in the three treatments. Antioxidant activities significantly increased in the Se0.6 and Se2 treatments. Low Se contents (0.6, 2 mg/kg) promoted root growth, including dry weight, length, surface area, volume, and root activity. There was a significant linear relationship between root and aboveground Se contents. The Se translocation factor increased as the soil Se content increased, ranging from 0.173 to 0.288. The application of Se can improve the state of rhizosphere soil’s organic C and soil nutrients (N, P, and K). Se significantly promoted the methane oxidation rate in rhizosphere soils, and the Se10 treatment showed the highest methane oxidation rate. The soil Se gradients led to differentiation in the growth, rhizosphere soil properties, and methane oxidation capacity of prickly pear. The root Se content and Se translocation factor were significantly positively correlated with the methane oxidation rate. Prickly pear can accumulate Se when grown in Se-enriched soil. The 2 mg/kg Se soil treatment enhanced growth and methane oxidation in the rhizosphere soil of prickly pear.

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Methanotrophs Contribute to Nitrogen Fixation in Emergent Macrophytes

Cited by 14 publications

References 54 publications

Ferrihydrite-mediated methanotrophic nitrogen fixation in paddy soil under hypoxia

Ferrihydrite-mediated methanotrophic nitrogen fixation in paddy soil under hypoxia

Methanotrophic Inoculation Reduces Methane Emissions from Rice Cultivation Supplied with Pig-Livestock Biogas Digestive Effluent

Selenium-Induced Enhancement in Growth and Rhizosphere Soil Methane Oxidation of Prickly Pear

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