Methicillin‐Resistant Coagulase‐Negative Staphylococci in the Community: High Homology of SCCmec IVa between<i>Staphylococcus epidermidis</i>and Major Clones of Methicillin‐Resistant<i>Staphylococcus aureus</i>

Barbier, François; Ruppé, Étienne; Hernández, David; Lebeaux, David; François, Patrice; Félix, Benjamin; Desprez, Adeline; Maiga, Aminata; Woerther, Paul‐Louis; Gaillard, K.; Jeanrot, C.; Wolff, Michel; Schrenzel, Jacques; Andremont, Antoine; Ruimy, Raymond

doi:10.1086/653483

Cited by 143 publications

(104 citation statements)

References 49 publications

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“…These findings, in accordance with other studies, confirm that S. haemolyticus can be considered as a reservoir of ccrC and a source for generating SCCmec VII (Ruppe et al, 2009;Urushibara et al, 2011;Bouchami et al, 2012). In a similar way, MR S. epidermidis appears to be a reservoir of ccrB and SCCmec IV (Ruppe et al, 2009;Barbier et al, 2010;Bloemendaal et al, 2010). With these observations, we suggest that the type of SCCmec is defined by species where it was generated.…”

Section: Discussionsupporting

confidence: 92%

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Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA

Djoudi

Bonura

Touati

et al. 2016

Journal of Medical Microbiology

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Genetic mechanisms of methicillin resistance are still relevant in staphylococci. The aims of this study are to assess the possible exchanges of staphylococcal cassette chromosome mec (SCCmec) among isolates of methicillin-resistant staphylococci (MRS) and to check for known or new mutations in mecA DNA. A total of 35 MRS non-repetitive isolates were recovered, including 20 Staphylococcus haemolyticus, 7 Staphylococcus aureus, 4 Staphylococcus sciuri, 2 Staphylococcus saprophyticus and 1 isolate each of Staphylococcus xylosus and Staphylococcus lentus. Only 16 of the 35 strains were assigned to known SCCmec types: 7 SCCmec VII, 6 SCCmec IV and 3 SCCmec III, with possible horizontal transfer of the SCCmec VII from methicillin-resistant S. haemolyticus to methicillin-susceptible S. aureus. mecA gene sequencing in ten selected isolates allowed description of nine punctual mutations, seven of which were reported for the first time. The most frequent mutation was G246E, identified in isolates of methicillin-resistant S. aureus, S. sciuri, S. saprophyticus and S. lentus. These results emphasized the high degree of genetic diversity of SCCmec element in MRS and describe new missense mutations in mecA, which might be important in understanding the evolution of methicillin and new b-lactam resistance.

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Section: Discussionsupporting

confidence: 92%

“…MR S. haemolyticus is the more frequently isolated species. Indeed, according to De Mattos et al (2003) and Barbier et al (2010), 23 % and 12 % of isolates belonged to this species, respectively. Barros et al (2011) have considered S. haemolyticus as an important hospital pathogen, as well as a carrier of methicillin resistance genes.…”

Section: Discussionmentioning

confidence: 86%

Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA

Djoudi

Bonura

Touati

et al. 2016

Journal of Medical Microbiology

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“…agr type III was found in ten (17%) of this study isolates, which was significantly lower than 49% in France (30), higher than 0% reported in United State of America (34), and had no difference with 12.5% reported in China (35). In this study, ten isolates were not typeable using primers, which was significantly higher than 0% and 2% reported in United State of America (34) Given that the strains of the present study were collected from the ICU, the results of this study are similar to a study conducted in Sweden (16), while they are different from other studies (17,36,37), in which nasal isolates or isolates with different sources were investigated.…”

Section: Discussioncontrasting

confidence: 62%

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

Peerayeh

Behmanesh

Moghadas

2018

Arch Clin Infect Dis

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Background: Quorum-sensing systems are considered as important mechanisms for pathogenesis and bacterial communication. The accessory gene regulator (agr) is one of the quorum-sensing systems in staphylococci, which is generally conserved. It is believed that there is a correlation between agr groups and infection. Multiple-locus VNTR analysis is a method for bacterial typing, previously applied for several different species of bacteria. This study aimed at determining the diversity of Staphylococcus epidermidis accessory gene regulator and clonality in clinical isolates from intensive care unit patients, Tehran, Iran. Methods: A total of 59 Staphylococcus epidermidis isolates were obtained from intensive care unit patients. The MLVA was performed for S. epidermidis isolates, using seven VNTR loci, including SE2395, SE0331, SE 828, SE1632, SE0175, Se2, and Se4. Specific primers were used for agr diversity determination. Results: The agr type I was observed in 29 (49%), while each of the agr type II and agr type III were observed in 10 (17%). Furthermore, 10 (17%) isolates were untypeable with using primers. In total, 49 MLVA genotypes were discriminated. Isolates were classified to six clonal complexes. Of the 59 isolate, 33 were included in clonal complex 1 (CC1), the largest of which harbored 15 (45.4%) agr type I. Conclusions: Agr type I was observed in the majority of the isolates. The MLVA results of this study suggest that there was a clone with 33 samples, comprised of 56% of isolates; smaller clones each comprised of two to seven isolates, and four isolates in the form of singleton. It seems that big clone isolates were settled in the intensive care unit (ICU), and singleton isolates entered the ward by visitors or medical personnel and caused infection.

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“…Resistance to these antibiotics has been increasingly reported in CNS associated with bovine mastitis (Walther and Perreten, 2007;Sawant et al, 2009;Sampimon et al, 2011). The CNS may also harbor antimicrobial resistance elements and pathogenicity islands, such as the staphylococcal cassette chromosome (SCCmec) element (Wielders et al, 2001;Barbier et al, 2010;Tsubakishita et al, 2010) and the arginine catabolic mobile element (ACME; Diep et al, 2006Diep et al, , 2008Miragaia et al, 2009) that can be transferred to Staph. aureus.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk

Frey

Rodríguez

Thomann

et al. 2013

Journal of Dairy Science

141

100

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Coagulase-negative staphylococci (CNS; n = 417) were isolated from bovine milk and identified by matrix-assisted laser desorption/ionization time-offlight mass spectrometry. Nineteen different species were identified, and Staphylococcus xylosus, Staphylococcus chromogenes, Staphylococcus haemolyticus, and Staphylococcus sciuri were the most prevalent species. Resistance to oxacillin (47.0% of the isolates), fusidic acid (33.8%), tiamulin (31.9%), penicillin (23.3%), tetracycline (15.8%), streptomycin (9.6%), erythromycin (7.0%), sulfonamides (5%), trimethoprim (4.3%), clindamycin (3.4%), kanamycin (2.4%), and gentamicin (2.4%) was detected. Resistance to oxacillin was attributed to the mecA gene in 9.7% of the oxacillin-resistant isolates. The remaining oxacillin-resistant CNS did not contain the mecC gene or mecA1 promoter mutations. The mecA gene was detected in Staphylococcus fleurettii, Staphylococcus epidermidis, Staph. haemolyticus, and Staph. xylosus. Resistance to tetracycline was attributed to the presence of tet(K) and tet(L), penicillin resistance to blaZ, streptomycin resistance to str and ant(6)-Ia, and erythromycin resistance to erm(C), erm(B), and msr. Resistance to tiamulin and fusidic acid could not be attributed to an acquired resistance gene. In total, 15.1% of the CNS isolates were multidrug resistant (i.e., resistant to 2 or more antimicrobials). The remaining CNS isolates were susceptible to antimicrobials commonly used in mastitis treatment. Methicillin-resistant CNS isolates were diverse, as determined by mecA gene sequence analysis, staphylococcal cassette chromosome mec typing, and pulsed-field gel electrophoresis. Arginine catabolic mobile element types 1 and 3 were detected in both methicillin-resistant and methicillin-susceptible Staph. epidermidis and were associated with sequence types ST59 and ST111. Because this study revealed the presence of multidrugresistant CNS in a heterogeneous CNS population, we recommend antibiogram analysis of CNS in persistent infections before treatment with antimicrobials.

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Methicillin‐Resistant Coagulase‐Negative Staphylococci in the Community: High Homology of SCCmec IVa betweenStaphylococcus epidermidisand Major Clones of Methicillin‐ResistantStaphylococcus aureus

Abstract: MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRSA.

Cited by 143 publications

References 49 publications

Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA

Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA

Staphylococcus epidermidis, Clonality and Accessory Gene Regulator Diversity in Clinical Isolates

Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk

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