Methimazole Is an Inhibitor of Melanin Synthesis in Cultured B16 Melanocytes

Kasraee, Behrooz; Hügin, Ambros W.; Tran, Christian; Sorg, Olivier; Saurat, Jean‐Hilaire

doi:10.1111/j.0022-202x.2004.22509.x

Cited by 37 publications

(56 citation statements)

References 21 publications

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“…Melanin levels were determined from cell extracts as previously described by Kasraee et al 11 and Kinoshita et al 12 Briefly, 5.0ϫ 10 6 cells/ ml cells were removed from the flask by trypsinization and dissolved in 1N NaOH. After 1 h incubation at 37°C, the solution was centrifuged and the absorbance of the extract was measured at 405 nm.…”

Section: F Biological Tests On Clonesmentioning

confidence: 99%

Dielectrophoretic separation of mouse melanoma clones

et al. 2010

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Dielectrophoresis ͑DEP͒ is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

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Section: F Biological Tests On Clonesmentioning

confidence: 99%

Dielectrophoretic separation of mouse melanoma clones

et al. 2010

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“…A significant influence of methimazole on melanocyte functions appears (17,19) despite the lack of cytotoxicity even in high concentrations (2). This phenomenon is explained by post-drug blocking of peroxidase, present in melanocytes, which takes part in various phases of melanogenesis (18). Methimazole, similarly to other thioamids, reduces the expression of proliferative cell nuclear antigen (PCNA) in the examined bioptates.…”

Section: Discussionmentioning

confidence: 92%

Biophysical parameters of rats’ skin after the administration of methimazole

Gołyński¹,

Szczepanik²,

Lutnicki³

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The paper describes the influence of oral administration of methimazole on biophysical skin parameters. Wistar rats of different sex (220-260 g) were used in the experiment. Biophysical skin parameters, such as transepidermal water loss (TEWL), corneometry, and pH were examined at seven-day intervals. Significant changes in the parameters were observed on the 7 th d of methimazole administration. The changes were observed in both sex but males appeared to be less sensitive in that respect. Changes in the parameters in the females showed rapid mechanisms, which normalised transepidermal water loss and skin hydration, as well as restored skin barrier functions. TEWL, skin hydration, and skin pH measurements allow an early assessment of skin barrier dysfunction after administration of this drug.

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“…Cell cultures and reconstructed epidermis were rinsed with phosphate buffered saline (PBS), treated with trypsin 0.05%-EDTA for 10 minutes at 37°C or with trypsin 0.83% for one hour at 37°C, respectively, then melanin was extracted and its content determined spectrophotometrically at 405 nm and normalised by MTT assay as previously described [16].…”

Section: Melanin Determinationmentioning

confidence: 99%

“…For cell viability the measured parameter was the value for MTT assay (i.e. MTT formazan, or MTTF) and for cellular melanin content the measured parameter was expressed as (µg melanin/µmol MTTF) [16]. From the corresponding concentration-responses curves the IC50s for the toxicity and the depigmentation can be determined.…”

Section: Tolerance Profiles Of Retinoid Derivatives In Keratinocytes mentioning

confidence: 99%

In Vitro Assessment of the Tolerance Profile, the Metabolism and the Potential Depigmenting Activity of New Retinoid Derivatives

Sorg¹,

Kasraee²,

Saurat³

2014

TODDISJ

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Abstract:Background: In spite of the numerous depigmenting agents available, there is an inverse relationship between safety and efficiency. Natural retinoids exert depigmenting activities in vivo, but have a narrow therapeutic window. There is thus a need for a new class of depigmenting agents that would be both safe and useful.Objective: The aim of the study was to analyse the potential interest of new retinoid derivatives as topical depigmenting agents. Methods:The tolerance profile of the new retinoids was analysed in murine BDVII keratinocytes and murine B16 melanocytes using the MTT assay. The uptake and the metabolism of the retinoids were analysed in keratinocytes and melanocytes by HPLC/UV. The cellular content of melanin was determined in melanocytes using a photometric assay.Results: Methyl-and t-butyl-4-oxoretinoate, arbutin retinoate and retinoic acid were shown to have the best depigmenting potential according to the ratio between the IC 50 for MTT assay and that for melanin content. Retinaldehyde, 4-hydroxyretinaldehyde and methyl-4-hydroxyretinoate have a good depigmenting potential too, although the therapeutic window is narrower due to a higher toxicity.Conclusion: 4-Oxoesters of retinoic acid, arbutin retinoate and 4-hydroxyretinaldehyde derivatives offer a new family of depigmenting agents acting via a different pathway than tyrosinase inhibitors.

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Methimazole Is an Inhibitor of Melanin Synthesis in Cultured B16 Melanocytes

Cited by 37 publications

References 21 publications

Dielectrophoretic separation of mouse melanoma clones

Dielectrophoretic separation of mouse melanoma clones

Biophysical parameters of rats’ skin after the administration of methimazole

In Vitro Assessment of the Tolerance Profile, the Metabolism and the Potential Depigmenting Activity of New Retinoid Derivatives

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