Method comparison between Munich II and III nomenclature for Pap smear samples

Cirkel, Christoph; Barop, Claudia; Beyer, Dániel

doi:10.5152/jtgga.2015.0147

Cited by 8 publications

(10 citation statements)

References 8 publications

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“…Anal smears were stained with Papanicolaou reagent and then evaluated by a PAP‐trained pathology specialist. The diagnostic classification was performed according to the Munich Nomenclature II (Table ) …”

Section: Methodsmentioning

confidence: 99%

“…The diagnostic classification was performed according to the Munich Nomenclature II (Table 1). 25 Anoscopy A board-certified specialist for proctology performed conventional diagnostic anoscopies. Clinical evaluation and mucosa biopsies from suspicious regions were performed upon staining of the suspicious areas with 3% acetic acid using a 4 mm skin biopsy punch (Stiefel Laboratorium AG, Offenbach, Germany).…”

Section: Ain Cytobrush Screening and Anal Cytology By Pap Smear Assesmentioning

confidence: 99%

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Evaluation of anal carcinoma screening in male and female HIV patients at an interdisciplinary HIV therapy centre

Todorova

Schwierzeck

Turek

et al. 2019

Acad Dermatol Venereol

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Background Incidence of anal carcinoma is increased in people living with HIV (PLWH). Due to the improved life expectancy in PLWH, identifying appropriate prevention strategies for non‐AIDS‐defining cancer types such as anal carcinoma has become a priority in managing PLWH today. Objective We aimed to evaluate anal cytology assessment as screening tool for anal dysplasia and/or carcinoma in PLWH, regardless of gender or sexual orientation. Additionally, we investigated the correlation between cancer risk factors and abnormal screening results in our patient cohort. Methods People living with HIV from the Interdisciplinary HIV Centre of the University Hospital rechts der Isar in Munich, Germany (IZAR), were screened for anal carcinoma by single cytobrush examination and anal Papanicolaou (PAP) smear assessment from 2013 to 2015. Patients with abnormal PAP smear result were offered a follow‐up examination after 12 months. Differences between two groups were tested for statistical significance using Student's t‐test and Mann–Whitney U‐test, as appropriate. Results In total, 101 PLWH were included. 26.7% of subjects (n = 27) were PAP IIID, and 9.9% (n = 10) were PAP IVa. Seven female subjects had an abnormal finding at screening. Smoking was significantly associated with abnormal findings at screening (P = 0.005). In addition, our study found an association between sexually transmitted infections (STI) and anal dysplasia. Condylomata acuminata were increased in subjects with PAP IIID/PAP IVa (P = 0.045). Reactive syphilis serology was found to be significantly associated with abnormal screening results (P = 0.016), respectively. Conclusion Our results demonstrate that smoking and two common STIs, condylomata acuminata and syphilis, are risk factors associated with advanced anal intraepithelial neoplasia (AIN) stages in our PLWH cohort. While further analysis is needed to determine diagnostic guidelines concerning AIN in PLWH, these results suggest that interdisciplinary lifestyle prevention strategies are required to reduce the risk factors for AIN in PLWH in an outpatient setting.

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Section: Methodsmentioning

confidence: 99%

Section: Ain Cytobrush Screening and Anal Cytology By Pap Smear Assesmentioning

confidence: 99%

Evaluation of anal carcinoma screening in male and female HIV patients at an interdisciplinary HIV therapy centre

Todorova

Schwierzeck

Turek

et al. 2019

Acad Dermatol Venereol

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“…Cytological examinations were interpreted according to Munich Nomenclature for the Cytological Diagnosis of Cervical Pap smears [ 17 ]. In our study cytological findings were classified as abnormal (PAP III+) if the pathologist reported PAP III (unclear finding corresponding in the Bethesda classification system to ASCUS), PAP IIID (cells of mild or moderate dysplasia corresponding in the Bethesda classification system to LSIL or HSIL respectively), PAP IVA (cells of severe dysplasia or carcinoma in situ corresponding in the Bethesda classification system to HSIL or AIS, respectively), PAP IVB (cells of invasive carcinoma not safely excluded, corresponding in the Bethesda classification system to HSIL or AIS with features suspicious for invasion, respectively) or PAP V (cells of invasive cervical carcinoma).…”

Section: Methodsmentioning

confidence: 99%

Pre-vaccine era cervical human papillomavirus infection among screening population of women in west Austria

Borena¹,

Grünberger²,

Widschwendter³

et al. 2016

BMC Public Health

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BackgroundIn order to evaluate the newly implemented gender-neutral HPV vaccination program, knowledge on the pre-vaccine prevalence of HPV infection is of paramount importance. Data on HPV infection among the women with no known previous cytological abnormalities are inexistent in Austria. This study presents data on the prevalence and distribution of HPV genotypes among women with no known cytological abnormalities in west Austria.MethodsWomen between 18 and 65 years of age attending annual cervical cancer screening examinations were included in the study. Data on socio-demographic and reproductive factors were collected using structured questionnaires. Corresponding cervical swab samples were tested for the presence of HPV DNA and were genotyped. Questionnaire data and HPV status were linked with the corresponding cytological findings.ResultsA total of 542 women were included in the study. The mean age of the study participants was 35.9 (SD = 11.5). The prevalence of HPV infection was 20.5 %. HPV 16 (6.5 %), HPV 33 (3.3 %) and HPV 31 (3.0 %) were the dominant genotypes detected. Multivariate analysis showed that women younger than 30 years of age, smokers, women with a higher number of lifetime sexual partners and those living in the eastern districts of the study region were at significantly higher risk of HPV infection.ConclusionsWith this study we present the first data on the prevalence of cervical HPV genotypes among a screening population in Austria. The results not only fill the missing information on HPV infection in this group of women in the country, they also provide baseline data for a future evaluation of the impact of the Austrian gender-neutral HPV immunization program. Moreover, our finding of higher HPV prevalence in the eastern compared to the western district of the study region may – at least partly – explain the east–west gradient in the standardized incidence rate of cervical cancer in the region.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-016-3581-0) contains supplementary material, which is available to authorized users.

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“…Raman imaging was used to analyze human epithelial cervical cells obtained from 96 patients classified to eight groups according to Munich III system [51] and HPV tests: I/HPV − , IIa/HPV − , IIa/HPV + , LSIL/HPV − , LSIL/HPV + , HSIL/HPV − , HSIL/HPV + and cancer cells -squamous cell carcinoma (SCC/HPV + ; for number of patients see Table S1). The cluster analysis (CA, a statistical method enabling to classify spectra according to a chemical composition) was used to determine distribution of main subcellular structures.…”

Section: Subcellular Distribution Of Glycogen In Cervical Epithelial Cellsmentioning

confidence: 99%

“…As HPV induces activation of various signal transduction pathways related to metabolic activity of cervical cells, in this work, Raman microscopy with subcellular resolution has been used to study distribution of chemical components in epithelial cervical cells collected from HPV positive or negative patients. Patients were classified according to the Munich III system [51,52] to I, IIa, IIID-1 IIID-2 (IIID-1 and IIID-2 corresponding to Bethesda LSIL-low-grade squamous intraepithelial lesion and HSIL-high-grade squamous intraepithelial lesion groups, respectively) and cancer groups, in the followed text called I, IIa, LSIL and HSIL as Bethesda nomenclature is commonly used in the literature. Investigation of Raman data, supported by the chemometric data analysis and periodic acid-Schiff (PAS) staining, demonstrates that for cervical cells with large (diameter over 10 µm) cell nuclei, the level of glycogen is significantly decreased in comparison to low-diameter nuclei cells.…”

Section: Introductionmentioning

confidence: 99%

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

Sitarz

Czamara

Bialecka³

et al. 2020

IJMS

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Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV−, IIa/HPV−, IIa/HPV−, LSIL/HPV−, LSIL/HPV+, HSIL/HPV−, HSIL/HPV+ and cancer cells (SCC/HPV+). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics.

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Method comparison between Munich II and III nomenclature for Pap smear samples

Abstract: Keywords: Munich nomenclature II, Munich nomenclature III, Pap smear Received: 30 July, 2015 Accepted: 14 October, 2015 Method

Cited by 8 publications

References 8 publications

Evaluation of anal carcinoma screening in male and female HIV patients at an interdisciplinary HIV therapy centre

Evaluation of anal carcinoma screening in male and female HIV patients at an interdisciplinary HIV therapy centre

Pre-vaccine era cervical human papillomavirus infection among screening population of women in west Austria

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

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