Method Development and Optimization for Colchicine Estimation in Toxicological Analysis using Dispersive LiquidLiquid Micro-Extraction and HPLC-UV

Abstract

Background:Colchicine is an alkaloid with anti-mitotic properties and has a narrow Therapeutic Index which can be proven to be fatal at higher doses. The present study is an attempt to develop a method that includes an effective adaptation of Dispersive Liquid-Liquid Microextraction (DLLME) with optimized parameters. Methods:The preliminary analysis was performed with UV-VIS Spectrophotometer. The analysis was performed using a Shimadzu LC-10AT with SPD-10A UV-VIS Detector. The sample analyzed was Zycolchin 0.5mg. The developed method was validated in accordance with the ICH guidelines. Results:The LOD and LOQ was determined to be 6µg/ml and 19µg/ml respectively. The system suitability was determined using the symmetry and efficiency calculation. The sample showed excellent linearity of R 2 = 0.99. The present study facilitates an optimized methodology for extraction and analysis which ensures great efficiency for the forensic analysis of colchicine. The study also highlights the importance of microextractions as they require only micro-level solvent usage which greatly improves the laboratory analysis. The entire extraction process is quick and effective for the analyte and is useful for the toxicological analysis in the forensic perspective.