Method development and validation for isoflavones in soy germ pharmaceutical capsules using micellar electrokinetic chromatography

Micke, Gustavo Amadeu; Fujiya, Neide Mitsue; Tonin, Fernando Gustavo; Costa, Ana Carolina de Oliveira; Tavares, Marina Franco Maggi

doi:10.1016/j.jpba.2006.03.012

Cited by 31 publications

(13 citation statements)

References 35 publications

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“…The separation of six isoflavones (three aglycones and three glycosides) extracted from soy germ capsules was carried out by MEKC-UV with a BGE composed of 10 mM sodium tetraborate at pH 9.3, 40 mM SDS and 1% v/v methanol [54]. This method afforded the baseline separation of the analytes studied with good precision, i.e.…”

Section: Phenols Polyphenols and Pigmentsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

150

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Section: Phenols Polyphenols and Pigmentsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

150

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“…As already reported [6], soy isoflavones have been analysed by means of CE and in particular, a method based on MEKC has been published, reporting their analysis in soy extracts. This method was therefore chosen as a starting point for this work.…”

Section: Development Of the Mekc Methodsmentioning

confidence: 99%

“…Starting from a previous paper dealing with the analysis of soy isoflavones [6], a procedure for the extraction of both isoflavones and MLT was developed. Using this extraction procedure with a water/methanol (20:80 v/v) mixture for 2 h, MLT recovery was quite low (70%).…”

Section: Development Of the Extraction Proceduresmentioning

confidence: 99%

“…Several papers can be found in the literature, regarding the determination of soy isoflavones in formulations [6 -8], raw materials for formulation preparation [9], dietary supplements [10 -16] or infant formulae [17,18], by a variety of methods such as MEKC [6], gradient [11,12] or isocratic [7 -10, 14, 18] HPLC with UV detection, HPLC with mass spectrometric detection [13,16,17] and HPLC with coulometric electrode array detection [15].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of analytical methods for the quality control of a new formulation containing soy extract and melatonin

Saracino

Mercolini

Musenga

et al. 2008

J of Separation Science

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Three analytical methods have been developed and compared for the quality control of a new formulation (Soymen GN(R) capsules) containing soy extract and melatonin for the treatment of menopausal symptoms. The first method is based on MEKC with diode-array detection, using a mixture of basic carbonate buffer (95%) and methanol (5%), containing 55 mM SDS, as the BGE. The second method is an HPLC method with UV detection at 260 nm. The third method is an HPLC method coupled to amperometric detection which is carried out at an oxidation potential of +0.8 V. In both HPLC systems, the chromatographic separation is obtained on an RP C18 column using a mixture of ACN and an acidic phosphate buffer (25:75 v/v) as the mobile phase. A feasible pretreatment procedure with a methanol/water mixture has been implemented to achieve the quantitative extraction of the main soy isoflavones and of melatonin from the capsules. The results obtained with the three methods are in good agreement with each other and satisfactory in terms of linearity (r(2) >0.9996), precision (RSD <5.4%) and accuracy (recovery >97%). Thus, each of the three analytical methods seems to be suitable for the simultaneous analysis of the main soy isoflavones and melatonin in the new commercial formulation.

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“…Many articles have been written on the identification and quantification of isoflavones or phytoestrogens (Wang et al, 2002) in soy products (such as capsules of soy germ, and nutritional supplements or other products containing soy) using high-performance liquid chromatography (HPLC) detection method (Micke et al, 2006;Griffith & Collison, 2001), nuclear magnetic resonance spectroscopy (Xiao et al, 2005), and other chromatographic. However, only a few researchers (such as César et al, 2006) have developed methods for quantifying isoflavones in capsules.…”

Section: Introductionmentioning

confidence: 99%

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

Auwerter¹,

Wanczinski²,

Chiandotti³

2012

Rev. bras. farmacogn.

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Isoflavones can be found in grains and leaves of soybean. Currently, these are sold in pharmacies as phytotherapic capsules. Isoflavones have been recommended by doctors, especially for women, due to their ability to relieve menopause symptoms, among other benefits. However, no method exists for the official control of isoflavone content in capsules sold in the Brazilian market. This study aims to develop an appropriate analytical method to determine the total isoflavone content (daidzin, glycitin, and genistin, and their respective aglycone forms) in phytotherapic capsules purchased in pharmacies in Curitiba, Parana State, Brazil, using the technique of high-performance liquid chromatography with UV detection (UV-HPLC). The HPLC system consisted of a quaternary pump, an autosampler, and Waters reversed-phase C 18 column (5 μm × 300 mm. Analyses were carried out at 40 °C, using a flow rate of 1.0 mL/min (acetonitrile and acetic acid 0.1%), and detection was performed at 254 nm. The method was validated as required by ANVISA and showed to be reliable for the following parameters: linearity (r 2 >0.99), selectivity (correlation between 0.99 and 1.00), precision (relative standard derivation <1.59%), accuracy (from 80% to 111.63% intraday and from 80% to 117.88% interday recovery), and robustness.

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Method development and validation for isoflavones in soy germ pharmaceutical capsules using micellar electrokinetic chromatography

Cited by 31 publications

References 35 publications

Recent advances in the application of capillary electromigration methods for food analysis

Recent advances in the application of capillary electromigration methods for food analysis

Comparison of analytical methods for the quality control of a new formulation containing soy extract and melatonin

Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography

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