Method development and validation for the HPLC potency assay of troglitazone tablets

Determination of troglitazone stereoisomers in rat plasma using semi-micro HPLC with electrochemical detection

“…1. Some HPLC methods have been reported and validated for the analysis of troglitazone in pharmaceutical and biological samples [3][4][5][6], however, it would be impossible to detect each stereoisomer of troglitazone using these methods. In general, separation of stereoisomers has become very important in analytical chemistry, especially in the pharmaceutical and biological fields, because some stereoisomers of racemic drugs have quite different pharmacokinetic properties and different pharmacological or toxicological effects [7][8][9].…”