Method Development and Validation of a High-Performance Liquid Chromatographic Method for Pyrethrum Extract

Abstract

A robust analytical methodology is developed for the quantitative determination of all six insecticidal components of pyrethrum extract: jasmolin I, cinerin I, pyrethrin I, jasmolin II, cinerin II, and pyrethrin II. This method, based on the separation technique normal-phase high-performance liquid chromatography, offers selectivity, accuracy, precision, linearity, range, ruggedness, and robustness as well as efficiency and ease.

Efficient, Baseline Separation of Pyrethrins by Centrifugal Partition Chromatography

“…[5]. Until recently, purification of reference standards of pyrethrins directly from the extract have been described only a few times [6]. Rickett successfully used liquid-liquid partitioning on diatomaceous earth carrying nitromethane as the stationary phase, while hexane and carbon tetrachloride acted as the mobile phase, separating 270 mg of total pyrethrins in a 17-hour experiment [7].…”

“…Rickett successfully used liquid-liquid partitioning on diatomaceous earth carrying nitromethane as the stationary phase, while hexane and carbon tetrachloride acted as the mobile phase, separating 270 mg of total pyrethrins in a 17-hour experiment [7]. In 2001, separation of pyrethrins was achieved by preparative HPLC using silica gel column [6]. The method was 100 min long, and after multiple injections, the collected amount varied between 11.4 mg for jasmolin II (6) (smallest component) and 93.4 mg for pyrethrin I (1) (largest component).…”

“…Pyrethrin I (1), cinerin I (2), and jasmolin I (3) are esters of trans-chrysanthemic acid (7) and constitute group I. The pyrethrins II group are esters of carbomethoxychrysanthemic acid (pyrethric acid) (8) and comprise of pyrethrin II (4), cinerin II (5), and jasmolin II (6). The pyrethrins, cinerins, and jasmolins differs by the alkyl chain present in the alcohol portion, which is called pyrethrolone (9), cinerolone (10), and jasmolone (11), respectively ( Figure 1).…”

Antiparasitic, antiprotozoal, molluscicidal and insecticidal activity of medicinal plants (part 2) – plant based review

“…The three chrysanthemic acid esters are commonly referred as pyrethrins I, and pyrethric acid esters as pyrethrins II. Among these compounds pyrethrin I and pyrethrin II are the most predominant and active [42][43][44][45].…”

Direct On-Line Continuous Supercritical Fluid Extraction and HPLC of Aqueous Pyrethrins Solutions

“…The analysis by high-performance liquid chromatography (HPLC) is not as difficult as GC analysis, because no degradation of the samples during the analysis has been observed. Several papers describe the analysis and successful separation of all six active compounds in pyrethrum extract. − Wintersteiger and Ofner developed the method for determining pyrethrins together with piperonyl butoxide in spiked human plasma by solid-phase extraction (SPE) followed by off- and on-line HPLC analysis. Other papers describe solid-phase extraction of pyrethrins and piperonyl butoxide of tap water or milk .…”