Method development for acetyl octapeptide-3 analysis by liquid chromatography-tandem mass spectrometry

Abstract

Background: Acetyl octapeptide-3 (SNAP-8) is an antiaging peptide that is more effective than acetyl hexapeptide-3, which is more stable than botulinum toxin and effectively relieves facial wrinkles. Products containing SNAP-8 such as patch are producing, but analytical method has not been reported to determination of SNAP-8. Method: Mobile phase, collision energy, and desolvation line temperature were optimized, and mass spectral data set for SNAP-8 was newly constructed by liquid chromatography-triple quadrupole mass spectrometer in multiple reaction monitoring mode. Results: The developed method showed good linearity (r ≥ 0.9971) with limit of quantification of 0.0125 ng/mL, repeatability (% relative standard deviation = 0.02 to 0.12) and accuracy (% relative error = − 1.68 to 1.44) under optimal conditions. This method was successfully applied to a biodegradable microneedle patch loaded with SNAP-8. Conclusion: The present method for the quantification of SNAP-8 may be useful for quality control in the cosmetic fields.

The ICH guidelines in practices: Stress degradation studies on botulinum toxin and validation of developed stability-indicating HPLC method

“…In addition, analysis of a commercial formulation of botulinum toxin showed that the drug elutes without interfering peaks generated by excipients in the commercial product. [6][7][8][9][10][11] Therefore, we found the method results to be stable for different parameters.…”

Chemical and biological properties of anti-wrinkle peptide Argireline

“…An example of Argireline derivative is acetyl octapeptide-3 with the sequence: Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH 2 . This peptide is more effective in wrinkle improvement than Argireline [29].…”

“…3, structure B) determination in biodegradable microneedle patches. The sample preparation consisted of extraction with water and filtration, due to the relatively simple matrix of microneedle load [29].…”

“…B:Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH 2, C: Ac-Glu-Glu-Met-Gln-Arg-( 13 C 6 , 15 N 4 )Arg-NH 2, D: H-Glu-Glu-Met-Gln-Arg-( 13 C 6 , 15 N 4 ) Arg-NH 2, E: H-Asp-Glu-Met-Gln-Arg-Arg-NH 2 , F: H-Glu-Ala-Met-Gln-Arg-Arg-NH 2 , G: H-Glu-Ala-Met-Gln-Ala-Arg-NH 2,H: H-Glu-Ala-His-Gln-Arg-Arg-NH 2 , I: Ac-Glu(OMe)-Glu(OMe)-Met-Gln-Arg-Arg-NH 2 , J: Ac-Glu(OMe)-Glu(OMe)-Met-Gln-Arg(Boc) 2 -Arg(Boc) 2 -NH 2 , K: Ac-Glu(OMe)-Glu(OMe)-Met-Gln-Arg(Ac) 2 -Arg(Ac) 2 -NH 2. "N" represents isotope 15 N; "C": isotope 13 C Source: Own study, structures based on sequences presented in:[26,29,[30][31][32]…”