Method development for high performance liquid chromatography: novel organic modifiers and column packing conditions

Abstract

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The effect of column packing procedure on column end efficiency and on bed heterogeneity – Experiments with flow-reversal

“…By this technique, the slurry suspension of packing materials is in a reservoir, which is connected to the column. The packing solvent is passed under high pressure and high flow-rate into the reservoir to push the slurry on the column [3][4][5].…”