Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

Hingse, Swarali Shivaji; Digole, Shraddha Bhikaji; Annapure, Uday S.

doi:10.1186/s40543-014-0021-6

Cited by 37 publications

(15 citation statements)

References 29 publications

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“…Accuracy in the experiments showed a mean recovery percentage in the range of 101.95–103.86% for vanillin, 100.67–114.93% for coniferyl aldehyde, and 94.06–101.15% for eugenol (Table ). The percentage recovery of 100% indicates that the developed method is constant and proportionate with no systematic error . In the current study, the percentage recoveries for the three metabolites were in the range of 94.06–114.93% with ≤2%RSD, thereby indicating the accuracy of the developed method for metabolites analysis.…”

Section: Resultssupporting

confidence: 57%

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

Singh

Sachan

Mukhopadhyay

et al. 2020

J of Separation Science

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An efficient ultra high performance liquid chromatography method of separation was developed for the analysis of six important methoxyphenol derivatives involved in the eugenol catabolic pathway. In the present study, an Acquity UPLC BEH C18 column was used for the chromatographic separation of the industrially important phenolic compounds such as vanillin, vanillic acid, ferulic acid, coniferyl alcohol, and coniferyl aldehyde obtained during microbial transformation of eugenol. Eluted components were identified using the dual wavelength (254 and 310 nm) UV detector. A gradient method of elution using mobile phase of aqueous 1 mM trifluoroacetic acid (Solvent A) and methanol (Solvent B) at a flow rate of 0.3 mL/min separated all the five intermediate methoxyphenol derivatives along with their precursor eugenol within 15 min with stable baseline resolution. Method validation was performed for the accurate quantification of vanillin, coniferyl aldehyde, and eugenol using the parameters of linearity, specificity, precision, limit of detection, limit of quantification, and robustness. The developed method would be helpful for clear separation and identification of the five most important intermediate metabolites of the eugenol catabolism pathway.

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Section: Resultssupporting

confidence: 57%

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

Singh

Sachan

Mukhopadhyay

et al. 2020

J of Separation Science

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“…The spectrophotometric assay is widely accepted, even though there is a concern about the spectral overlapping between substrate and product, e.g., methyl substrates and their aromatic acids. Recently, other methods have been developed such as high-performance thin layer chromatography and electrochemical sensor for rapid detection of ferulic acid which may be useful for enzymatic screening [73, 74]. …”

Section: Overview Of Substrate Specificity Of Characterized Faes and mentioning

confidence: 99%

Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications

Dilokpimol

Mäkelä

Pontes

et al. 2016

Biotechnol Biofuels

140

166

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Feruloyl esterases (FAEs) represent a diverse group of carboxyl esterases that specifically catalyze the hydrolysis of ester bonds between ferulic (hydroxycinnamic) acid and plant cell wall polysaccharides. Therefore, FAEs act as accessory enzymes to assist xylanolytic and pectinolytic enzymes in gaining access to their site of action during biomass conversion. Their ability to release ferulic acid and other hydroxycinnamic acids from plant biomass makes FAEs potential biocatalysts in a wide variety of applications such as in biofuel, food and feed, pulp and paper, cosmetics, and pharmaceutical industries. This review provides an updated overview of the knowledge on fungal FAEs, in particular describing their role in plant biomass degradation, diversity of their biochemical properties and substrate specificities, their regulation and conditions needed for their induction. Furthermore, the discovery of new FAEs using genome mining and phylogenetic analysis of current publicly accessible fungal genomes will also be presented. This has led to a new subfamily classification of fungal FAEs that takes into account both phylogeny and substrate specificity.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0651-6) contains supplementary material, which is available to authorized users.

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“…HPTLC is also proposed for analysis of ferulic acid. It is stated that this method is useful in both qualitative and quantitative analysis for routine assays in pharmaceutical and food industries within acceptable limits (Hingse et al 2014).…”

Section: Antimicrobial Effectmentioning

confidence: 99%

Ferulic acid in cereals - a review

Boz¹

2015

Czech J. Food Sci.

111

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Boz H. (2015): Ferulic acid in cereals -a review. Czech J. Food Sci., 33: 1-7.Phenolic acids represent the most common form of phenolic compounds in the cereal grain and many other plants. The phytochemical ferulic acid is found in the leaves and seeds of many plants, but especially in cereals. It is the most abundant phenolic acid in common cereals, representing up to 90% of total phenolic compounds. Ferulic acid has been reported to have many physiological functions, including antioxidant, antimicrobial, anti-inflammatory, and anti-cancer activities. It is an antioxidant which neutralises free radicals (superoxide, nitric oxide, and hydroxyl radical) which could cause oxidative damage to cell membranes and DNA. Like many other antioxidants, ferulic acid reduces the level of cholesterol and triglyceride, thereby reducing the risk of heart disease.

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Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

Cited by 37 publications

References 29 publications

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

A rapid and simple ultra high performance liquid chromatography method for the simultaneous determination of methoxyphenol derivatives involved in the eugenol catabolic pathway

Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications

Ferulic acid in cereals - a review

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