Method for Determination of Oxygen-18 Content of Inorganic Phosphate

Williams, Frank R.; Hager, Lowell P.

doi:10.1126/science.128.3336.1434

Cited by 29 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Crystalline potassium phosphate containing some KC1 was collected by filtration, washed with ethanol and ether, and dried in vacuo at 100°for 1-4 hours. 180 content of the orthophosphate was determined by the method of Williams and Hager (1958).…”

Section: Methodsmentioning

confidence: 99%

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Lardy¹,

Connelly²,

Johnson³

1964

Biochemistry

273

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Lardy¹,

Connelly²,

Johnson³

1964

Biochemistry

273

View full text Add to dashboard Cite

“…Three methods for the incorporation of oxygen from orthophosphate into C02 were used. The one used most regularly was that described by Williams and Hager (1958). A new method by Boyer et al (1961) was also used and worked equally well.…”

Section: Methodsmentioning

confidence: 99%

“…For this reason we compared the Williams and Hager (1958) and Boyer et al (1961) methods for analysis of the lsO content of phosphate (samples 3 and 4, Table V) and the Williams and Hager (1958) and Anbar and Guttmann (1959) methods (samples 5, 6, and 8, Table V). In addition, samples 5 and 6 were handled as indicated in the legend of Table V, to eliminate the possibility that our separation of the phosphate over the Dowex column leads to the retention of C18Oz in the phosphate samples.…”

Section: Fig 2•-mentioning

confidence: 99%

Decomposition of Carbamylphosphate in Aqueous Solutions^*

Allen¹,

Jones²

1964

Biochemistry

192

View full text Add to dashboard Cite

“…column of Florisil in a Pasteur pipet and all of the yellow product was collected. After removal of the benzene at reduced pressure, the sample was dried in vacuo for 24 hr. An nmr (CDCI3-TMS) indicated that the material was a mixture of imide and isoimide.…”

Section: Methodsmentioning

confidence: 99%

Oxygen-18 study of the reaction of N-phenylmaleamic acid with acetic anhydride

Sauers¹,

Gould²,

Ioannou³

1971

J. Org. Chem.

View full text Add to dashboard Cite

Benzoylation of 2a.-A solution of 2a (1 g, 0.00435 mol) and benzoyl chloride (1.4 g, 0.01 mol) in dry xylene (15 ml) was heated under reflux for 16 hr using a fiberglass heating mantle. The dark brown solution was diluted with benzene (10 ml), de-colorized with charcoal, and concentrated to dryness in a rotating evaporator under reduced pressure. The residual yellow oil (1.8 g) partially crystallized. Trituration with cold dry ether and collection at the filter gave 0.47 g (32% yield) of product, mp 145-150°. Recrystallization from methanol gave pure 10 (0.35 g, mp 158-160°) identical with the material obtained from, the pyrolysis of 2a. From the ethereal filtrate there was obtained an oil (0.47 g) which gave a colorless fraction (0.17 g) soluble in warm pentane. This oil could not be crystallized despite indications of virtual homogeneity by tic analysis. Although its infrared and mass spectra (M+ = 333.0339; caled for CnH^ChNCh: 333.0322) were nearly identical with the corresponding spectra of 10, the CHS singlet in the nmr appeared at 2.13 ppm instead of at 2.28 ppm for compound 10. Structure 12 for this oil was again ruled out by the following experiment.Benzoylation of 3a.-Application of the foregoing procedure to 1.31 g (0.0057 mol) of 3a gave a dark oil (1.8 g) that crystallized upon trituration with ether. Collection at the filter gave 1.05 g (mp 108-111°, 55% yield) of crude product. Several recrystallizations from methanol gave pure 0-benzoyl-A-(2,2dichloroisopropenyl)benzimidate ( 12): mp 111-113°; ir (CIICU) 1700 cm"1 (C=0), and no NH; nmr (CDC13) 8.3-7.3 (m, 10, ArH), and 2.13 ppm (s, 3, CHS).

show abstract

Method for Determination of Oxygen-18 Content of Inorganic Phosphate

Cited by 29 publications

References 3 publications

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Decomposition of Carbamylphosphate in Aqueous Solutions^*

Oxygen-18 study of the reaction of N-phenylmaleamic acid with acetic anhydride

Contact Info

Product

Resources

About

Method for Determination of Oxygen-18 Content of Inorganic Phosphate

Cited by 29 publications

References 3 publications

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin*

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin*

Decomposition of Carbamylphosphate in Aqueous Solutions*

Oxygen-18 study of the reaction of N-phenylmaleamic acid with acetic anhydride

Contact Info

Product

Resources

About

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Antibiotics as Tools for Metabolic Studies. II. Inhibition of Phosphoryl Transfer in Mitochondria by Oligomycin and Aurovertin^*

Decomposition of Carbamylphosphate in Aqueous Solutions^*