Method for determination of the reaction kinetics of an enzymatic conversion in a heterogeneous system

Krause, Przemyslaw; Macías, Roberto; Fieg, Georg; Gutsche, Bernhard

doi:10.1016/s1570-7946(09)70180-4

Cited by 2 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With regard to the stage number in a complex mixer−settler arrangement, the choice often emphasizes the use of higher solvent flows instead of increased number of stages, unless the cost of the solvent is restrictive. An experimental setup comprised of a mixing unit and a packed-bed reactor containing immobilized enzymes is under investigation . Other configurations are also possible and have been examined; however, they are not discussed here.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Applicability of a Countercurrent Enzymatically Catalyzed Multistage Extractive Reaction Process for the Hydrolysis of Methyl Octanoate

Krause

Macías

Fieg

2010

Ind. Eng. Chem. Res.

Self Cite

View full text Add to dashboard Cite

An assessment of the minimum requirements for a countercurrent multistage extractive reaction process for the enzymatic hydrolysis of methyl octanoate with immobilized lipase in undiluted media was made. For simulation, UNIQUAC parameters for the quaternary reaction system containing methyl octanoate, octanoic acid, methanol, and water at 48.5 °C and atmospheric pressure and the equilibrium conversion, as a function of the initial water content, were determined. For a one-equilibrium stage reaction, good overall agreement, with respect to the liquid-phase compositions between experiments, and results from chemical and phase equilibrium calculations, based on Gibbs free-energy minimization, was obtained. A subsequently performed analysis of the influence of the stage number and solvent:feed ratio, using commercial flowsheet simulation software, showed that, in a countercurrent process, five equilibrium stages are required to obtain octanoic acid with a purity exceeding mass fractions of 0.95 at comparably moderate water excess.

show abstract

Section: Resultsmentioning

confidence: 99%

“…An experimental setup comprised of a mixing unit and a packed-bed reactor containing immobilized enzymes is under investigation. 12 Other configurations are also possible and have been examined; however, they are not discussed here.…”

Section: Design Of the Mser Processmentioning

confidence: 99%

Applicability of a Countercurrent Enzymatically Catalyzed Multistage Extractive Reaction Process for the Hydrolysis of Methyl Octanoate

Krause

Macías

Fieg

2010

Ind. Eng. Chem. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…“The genetic algorithm (GA) is an optimization and search technique based on the principles of genetics and natural selection.” The genetic algorithm was used to determine the optimum kinetic parameters by minimizing the sum of squares of the error between the experimental (C exp ) and calculated (C cal ) substrate concentrations using the objective function given as follows:

O F = \sum_{j = 1}^{normaln} {[{normalC}_{c a l, j} - {normalC}_{\exp, j}]}^{2}

where n is the number of experimental data points. Adequate initial estimates for the kinetic parameters are required to obtain correct results …”

Section: Methodsmentioning

confidence: 99%

“…Adequate initial estimates for the kinetic parameters are required to obtain correct results. [43] KINETIC STUDIES Transesterification occurs in a three-step consecutive reaction in which 3 mol ester and 1 mol glycerol are produced according to the following reaction:…”

Section: Genetic Algorithmmentioning

confidence: 99%

Kinetic modelling of enzymatic biodiesel production from castor oil: Temperature dependence of the Ping Pong parameters

2016

View full text Add to dashboard Cite

Biodiesel is considered an alternative replacement for petroleum diesel, and enzymatic reaction is one method for biodiesel production. Understanding the kinetics of this reaction is important to achieve higher production rates. The kinetics of transesterification of castor oil using Novozym 435 was investigated in this study. The genetic algorithm was employed for estimation of kinetic parameters including Vmax, KmTG, and KmA in the Ping Pong kinetic model at different temperatures. In most enzymatic reactions that follow the Ping Pong mechanism, inhibition by alcohol has been taken into consideration. Here, we examined a competitive inhibition mechanism and found no inhibition effect at high alcohol‐to‐oil molar ratios of 12.6, 15.6, and 18.9 mol/mol. Arrhenius expressions were used to fit the kinetic parameters for reaction temperatures of 30–50 °C. An acceptable agreement with experimental data at 45 °C was observed when biodiesel yields were predicted using the temperature‐dependent parameters, thus validating the proposed model.

show abstract

Method for determination of the reaction kinetics of an enzymatic conversion in a heterogeneous system

Cited by 2 publications

References 4 publications

Applicability of a Countercurrent Enzymatically Catalyzed Multistage Extractive Reaction Process for the Hydrolysis of Methyl Octanoate

Applicability of a Countercurrent Enzymatically Catalyzed Multistage Extractive Reaction Process for the Hydrolysis of Methyl Octanoate

Kinetic modelling of enzymatic biodiesel production from castor oil: Temperature dependence of the Ping Pong parameters

Contact Info

Product

Resources

About