Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentration

Guarino, Richard D.; Dike, Laura E.; Haq, Tariq; Rowley, Jon A.; Pitner, J. Bruce; Timmins, Mark R.

doi:10.1002/bit.20613

Cited by 42 publications

(73 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To enable a direct comparison between the growth of cells on the substrate and within the gel, we have chosen to keep the initial density of 100,000 cells/ml for all samples in the experiments. The calculated values of OURs for the Hep-G2 cells is in agreement with the OUR of rat hepatocytes obtained from published literature (Balis et al 1999;Guarino et al 2003;Chandel et al 1997;De Bartolo et al 1999;Park 2002;Schumacker et al 1993;Smith et al 1996). The values seen in the literature also tend to vary greatly depending on the method of cell culture, time period of cell culture and method of measuring OUR.…”

Section: Discussionsupporting

confidence: 88%

“…4). Liver cells tend to be metabolically more active when cultured in higher concentrations where cell to cell contact are enhanced and hence have higher OUR values (Guarino et al 2003). In their native state, each liver cell borders a vascular space (sinusoid) on at least one side and with other hepatocytes on the remaining sides (Gartner and ed 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Most commonly used dyes in biological monitoring applications are the ruthenium-based dyes, due to its ease of availability, nontoxicity and stable properties. For measuring key analytes in tissue microenvironment, sol-gel films containing the dye is employed to clad the core of an optical fiber (Campbell and Uttamchandani 2004;Khijwania and Gupta 1999;O'Keeffe et al 1995;Tang et al 2003), as a patch attached to the bottom of glass spinner (Guarino et al 2003;Lung et al 2004) and as a tip of an optical probe (Khohls and Scherer 2000). The compact size and ability of fiber optic sensors to directly measure dissolved oxygen in the culture medium without the additional use of reagents make them ideal for monitoring tissue growth in real time (Park 2002;Rotem et al 1992Rotem et al , 1993.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Real time in vitro measurement of oxygen uptake rates for HEPG2 liver cells encapsulated in alginate matrices

Mishra

Starly

2009

Microfluid Nanofluid

View full text Add to dashboard Cite

Quantitative non-invasive measurement of critical physiological parameters is necessary to assess the functionality and applicability of tissue engineered matrices. Advancements in fiber optic sensors have made it possible for measuring parameters such as oxygen, glucose, and aminoacids necessary for viable tissue growth. In this study, we have devised an experimental protocol to measure in real time, the oxygen uptake rate (OUR) values for a selected liver cell line (HEPG2) when grown (a) on cover glass slides, and (b) encapsulated within alginate based hydrogel matrices. For both cases, the oxygen uptake rates of HEPG2 cells at selected time points varied in close co-relation with cell proliferation and metabolic activity during the 7-day culture period. This investigation concludes that OUR can be used as an indicative parameter to assess the metabolic activity of cells encapsulated within a matrix. The study also presents a fiber optic sensing technology as a non-invasive diagnostic tool to monitor cell behavior and activity.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real time in vitro measurement of oxygen uptake rates for HEPG2 liver cells encapsulated in alginate matrices

Mishra

Starly

2009

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…1b, d), and from day 3 the dividing cells form clusters of many individuals. Human embryonic lung fibroblasts [42] 2.5 9 10 -18…”

Section: Simple Model: Diffusion and Monodmentioning

confidence: 99%

A mathematical model for the design of fibrin microcapsules with skin cells

Acevedo

Weinstein‐Oppenheimer

Brown

et al. 2008

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

The use of fibrin in tissue engineering has greatly increased over the last 10 years. The aim of this research was to develop a mathematical model to relate the microcapsule-size and cell-load to growth and oxygen depletion. Keratinocytes were isolated from rat skins and microencapsulated dropping fibrinogen and thrombin solutions. The cell growth was measured with MTT-assay and confirmed using histochemical technique. The oxygen was evaluated using a Clark sensor. It was found that Fick-Monod model explained the cell growth for the first 48 h, but overestimated the same thereafter. It was necessary to add a logistic equation to reach valid results. In relation to the preferred implant alternative, when considering large initial cell loads, the possibility to implant small loads of fast-growing cells arises from the simulations. In relation to the microcapsule size, it was found that a critical diameter could be established from which cell growth velocity is about the same.

show abstract

“…This noncontact design enables easier sterilization procedures, but is limited to use in bioreactor containers with optically transparent surfaces. 27,46 A method to embed an optical sensor in a microfluidic chamber by using an integrated optical waveguide was developed to allow light to be effectively conducted to a select point of interest within the device with minimal interference. 50 An oxygen-sensitive fluorescent dye, tris (2,2¢-bipyridyl) dichlororuthenium(II)hexahydrate, combined with polymeric waveguides was fabricated on a glass substrate.…”

Section: Fluorescent Dyesmentioning

confidence: 99%

Enabling Sensor Technologies for the Quantitative Evaluation of Engineered Tissue

Starly

Choubey

2007

Ann Biomed Eng

View full text Add to dashboard Cite

Research in regenerative medicine has necessitated the need for advanced sensing technologies to monitor and evaluate the quality of engineered tissues. Several sensing schemes have been developed to sense specific analytes that enable researchers to assess tissue morphology, growth, and function. In addition to microscopy and staining techniques, tissue engineers are presented with an array of optical, chemical, and biological sensor technologies, which provide them with an opportunity to monitor variables, such as oxygen concentration, pH value, carbon dioxide, and glucose concentration in a noninvasive or minimally invasive manner. The article presents a short description on the core technologies and research reviews on the use of sensors employed in tissue engineering over the past decade. The article concludes by presenting some of the challenges to the further development of these technologies that are capable of real time measurement of tissue structure, composition, and function both for in-vitro and in-vivo analysis.

show abstract

Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentration

Abstract: The original article to which this Erratum refers was published in

Cited by 42 publications

References 0 publications

Real time in vitro measurement of oxygen uptake rates for HEPG2 liver cells encapsulated in alginate matrices

Real time in vitro measurement of oxygen uptake rates for HEPG2 liver cells encapsulated in alginate matrices

A mathematical model for the design of fibrin microcapsules with skin cells

Enabling Sensor Technologies for the Quantitative Evaluation of Engineered Tissue

Contact Info

Product

Resources

About