Method for efficient establishment of technical biodosimetry competence

Stricklin, Daniela; Jaworska, A; Arvidsson, E.

doi:10.1016/j.radmeas.2007.05.010

Cited by 6 publications

(1 citation statement)

References 4 publications

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“…These are termed cell fusion-induced and chemical/drug-induced PCC, respectively. Cell fusioninduced PCC is an alternative for dicentric analysis that has already been validated for biodosimetry purposes [8,9]; and chemical-induced PCC has frequently been used in high-dose estimation using ring aberrations as the endpoint for ionising radiations [10][11][12][13][14][15][16][17][18]. According to Kanda et al, PCC rings are a more suitable indicator than dicentrics at doses higher than 10 Gy [10].…”

Section: Introductionmentioning

confidence: 99%

A faster and easier biodosimetry method based on calyculin A-induced premature chromosome condensation (PCC) by scoring excess objects

Sun¹,

Moquet²,

Lloyd³

et al. 2020

J. Radiol. Prot.

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Dicentric analysis and the ring PCC assay as established biodosimetry methods both have limitations in the estimation of absorbed doses in suspected overexposure cases between 5 and 10 Gy. The proposed method based on calyculin A-induced PCC overcomes these limitations by scoring excess objects as the endpoint. This new scoring method can potentially serve as a faster and up-scalable approach that complements the existing methods with higher accuracy at different dose ranges. It can also potentially be performed by less skilled workers when no automated system is available in mass casualty emergency cases to assist with the triage of patients. Additionally, it offers the possibility to further reduce the sample size and PCC induction time. In this pilot study, a calibration curve for excess objects was constructed using the new scoring method for the first time and a blind validation test composed of three unknown doses was carried out. Almost all the dose estimates were within the 95% confidence limits of the actual test doses by scoring only 50–100 PCC spreads. This method was found to be more accurate than ring PCC for doses below 10 Gy.

show abstract

Section: Introductionmentioning

confidence: 99%

A faster and easier biodosimetry method based on calyculin A-induced premature chromosome condensation (PCC) by scoring excess objects

Sun¹,

Moquet²,

Lloyd³

et al. 2020

J. Radiol. Prot.

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show abstract

DNA Damage in Bat Blood Leukocytes Using a Chromatin Dispersion Test (CDT): Biomarker of Environmental Genotoxicity

Cortés-Gutiérrez,

Ceyca-Contreras,

Gómez-Ruiz

et al. 2024

Bull Environ Contam Toxicol

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Current status of biodosimetry based on standard cytogenetic methods

Pinto

Santos

Amaral

2010

Radiat Environ Biophys

118

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Knowledge about dose levels in radiation protection is an important step for risk assessment. However, in most cases of real or suspected accidental exposures to ionizing radiation (IR), physical dosimetry cannot be performed for retrospective estimates. In such situations, biological dosimetry has been proposed as an alternative for investigation. Briefly, biodosimetry can be defined as individual dose evaluation based on biological endpoints induced by IR (so-called biomarkers). The relationship between biological endpoints and absorbed dose is not always straightforward: nausea, vomiting and diarrhoea, for example, are the most well-known biological effects of individual irradiation, but a precise correlation between those symptoms and absorbed dose is hardly achieved. The scoring of unstable chromosomal-type aberrations (such as dicentrics and rings) and micronuclei in mitogen-stimulated peripheral blood, up till today, has been the most extensively biodosimetry assay employed for such purposes. Dicentric assay is the gold standard in biodosimetry, since its presence is generally considered to be specific to radiation exposure; scoring of micronuclei (a kind of by-product of chromosomal damages) is easier and faster than that of dicentrics for dose assessment. In this context, the aim of this work is to present an overview on biodosimetry based on standard cytogenetic methods, highlighting its advantages and limitations as tool in monitoring of radiation workers' doses or investigation into accidental exposures. Recent advances and perspectives are also briefly presented.

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Method for efficient establishment of technical biodosimetry competence

Cited by 6 publications

References 4 publications

A faster and easier biodosimetry method based on calyculin A-induced premature chromosome condensation (PCC) by scoring excess objects

A faster and easier biodosimetry method based on calyculin A-induced premature chromosome condensation (PCC) by scoring excess objects

DNA Damage in Bat Blood Leukocytes Using a Chromatin Dispersion Test (CDT): Biomarker of Environmental Genotoxicity

Current status of biodosimetry based on standard cytogenetic methods

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