Method for Measuring Mineralization in Lake Sediments

Harrison, Michael; Wright, Richard; Morita, Richard Y.

doi:10.1128/aem.21.4.698-702.1971

Cited by 80 publications

(23 citation statements)

References 7 publications

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“…Lower values (0.6-1.3 min) were observed in sediments of hypereutrophic Lake Wintergreen [9]. Higher values were found in sediments of Klamath Lake (135 min [25]) and Lake Kinnereth (210 min [26]).…”

Section: Discussionmentioning

confidence: 99%

Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sediment

Krumböck

Conrad

1991

FEMS Microbiology Ecology

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Section: Discussionmentioning

confidence: 99%

Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sediment

Krumböck

Conrad

1991

FEMS Microbiology Ecology

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“…The turnover times reported here are generally qua1 to or longer than those reported for aerobic uptake in lake or subtidal sediment slurries (Wood 1970;Hall ct al. 1972;IIarrison et al 1971;Wood and Chua 1973). In most studies samples were taken from the upper centimeter, although Wood (1970) did examine activity with depth and found longer turnover times with increasing depth.…”

Section: Discussionmentioning

confidence: 99%

“…In this and other studies (Wood 1970;IIarrison et al 1971;Wood and Chua 1973;Cappcnbcrg and Prins 1974) slurry systems have been used to determine the uptake of organic substrates. For studying subsurface sediments, the slurry technique is the only one available for rapid and uniform introduction of label (Christian and IIall 1977).…”

Section: Discussionmentioning

confidence: 99%

Anaerobic microbial community metabolism in Spartina alterniflora soil 1, 2

Christian

Wiebe

1978

Limnology & Oceanography

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The anaerobic uptake of [14C]glucose was used as an index of potential microbial activity in the soils of a Spartina alterniflora salt marsh. The turnover times of glucose were consistently faster in the soils where tall S. alterniflora grew along a creekbank than in the high marsh soils where S. alterniflora productivity was lower; this difference was magnified with increased depth. The distribution of label, followed through CO2, particulate, and ether‐soluble fractions indicated rapid recycling in the soil of the tall S. alterniflora marsh. In an experiment designed to assess the effects of tidal inundation on the activity of the microbial community in the tall S. alterniflora marsh soils, no consistent changes in glucose uptake or ATP concentration were evident after 2 months of restricting water movement. No direct link of tidal inundation with the microbial community was observed.

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“…Duplicate 0.5-ml aliquots of this culture were counted to obtain total culture disintegrations per minute. The rest of the culture was divided into one 6-ml aliquot and 10 5-ml aliquots in serum vials fitted with cap and bucket assemblies as described by Harrison et al (7). At desired intervals, duplicate 0.5-ml aliquots were withdrawn from the 6-ml culture and filtered through Gelman GN-6 filters (25 mm), which were counted to determine intracellular analog.…”

Section: Methodsmentioning

confidence: 99%

Role of protein synthesis in the survival of carbon-starved Escherichia coli K-12

Reeve¹,

Amy²,

Matin³

1984

J Bacteriol

197

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In a typical Escherichia coli K-12 culture starved for glucose, 50% of the cells lose viability in ca. 6 days (Reeve et al., J. Bacteriol. 157:758-763, 1984). Inhibition of protein synthesis by chloramphenicol resulted in a more rapid loss of viability in glucose-starved E. coli K-12 cultures. The more chloramphenicol added (i.e., the more protein synthesis was inhibited) and the earlier during starvation it was added, the greater was its effect on culture viability. Chloramphenicol was found to have the same effect on a reLA strain as on an isogenic reU+ strain of E. coli. Addition of the amino acid analogs S-2-aminoethylcysteine, 7-azatryptophan, and p-fluorophenylalanine to carbon-starved cultures to induce synthesis of abnormal proteins had an effect on viability similar to that observed when 50 ,ug of chloramphenicol per ml was added at zero time for starvation. Both chloramphenicol and the amino acid analogs had delayed effects on viability, compared with their effects on synthesis of normal proteins. The need for protein synthesis did not arise from cryptic growth, since no cryptic growth of the starving cells was observed under the conditions used. From these and previous results obtained from work with peptidase-deficient mutants of E. coli K-12 and Salmonella typhimurium LT2 (Reeve et al., J. Bacteriol. 157:758-763, 1984), we concluded that a number of survival-related proteins are synthesized by E. coli K-12 cells as a response to carbon starvation. These proteins are largely synthesized during the early hours of starvation, but their continued activity is required for long-term survival.Starving bacteria are of interest in both an ecological and an applied context, and we have been interested in characterizing the physiological changes that occur during starvation (12,16,22). We have previously presented evidence (16) that protein degradation plays a role in the survival of carbon-starved bacteria. Mutants of Escherichia coli and Salmonella typhimurium that were deficient in protein degradation were found to possess a greatly decreased stability under conditions of carbon starvation. Although these mutants had no innate deficiency in their protein-synthetic machinery, they were unable to synthesize protein at the same rate as their corresponding wild-type strains during carbon starvation. This suggested that amino acids derived from protein degradation were utilized by these cells for new protein synthesis and that their increased susceptibility to carbon starvation originated from their inability to synthesize these proteins.In E. coli cells subjected to carbon starvation, the rate of protein synthesis drops to about 20% of the initial rate during the first hour of starvation (16) and then remains roughly constant for at least the next 47 h (unpublished data). We present evidence here that this protein synthesis is important for the survival of carbon-starved E. coli K-12; inhibition of normal protein synthesis during starvation greatly compromised survival. MATERIALS AND METHODSBacterial strains, ...

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Method for Measuring Mineralization in Lake Sediments

Cited by 80 publications

References 7 publications

Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sediment

Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sediment

Anaerobic microbial community metabolism in Spartina alterniflora soil 1, 2

Role of protein synthesis in the survival of carbon-starved Escherichia coli K-12

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