2018
DOI: 10.1016/j.jviromet.2018.02.014
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Method for simple and rapid concentration of Zika virus particles from infected cell-culture supernatants

Abstract: Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33 ± 0.12 log DICT/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers.

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Cited by 3 publications
(2 citation statements)
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“…Flavivirus titers were determined in Vero cells using an immunoperoxidase assay using the anti-flavivirus group antigen antibody 4G2 (clone D1-4G2-4–15, ATCC, HB-112). Virus titers were calculated and expressed as 50% tissue culture infective dose per ml (TCID 50 /ml) using the Reed and Muench method [ 24 ].…”
Section: Methodsmentioning
confidence: 99%
“…Flavivirus titers were determined in Vero cells using an immunoperoxidase assay using the anti-flavivirus group antigen antibody 4G2 (clone D1-4G2-4–15, ATCC, HB-112). Virus titers were calculated and expressed as 50% tissue culture infective dose per ml (TCID 50 /ml) using the Reed and Muench method [ 24 ].…”
Section: Methodsmentioning
confidence: 99%
“…Experimental studies involving viruses often require viral stocks with high concentrations. While cell culture-based viral propagation systems are well developed for many viruses including CPV and FCoV, generating sufficient concentration of infective virus particles is not always possible by cellular propagation alone ( Ichim and Wells, 2011 , Richard and Aubry, 2018 ). Common virus concentration methods are based on high speed centrifugation, filtration, chemical precipitation, adsorption and affinity mechanisms; however, efficiency of the selected method is likely dependent on chemical and structural properties of the virus, condition of the sample/medium in which viral particles are retained, and the experimental purpose ( Ahmed et al, 2015 , Bofill-Mas and Rusiñol, 2020 , Ye et al, 2016 ).…”
mentioning
confidence: 99%