Method for Simultaneous Measurement of Antibodies to 23 Pneumococcal Capsular Polysaccharides

Biagini, Raymond E.; Schlottmann, Sonela A.; Sammons, Deborah L.; Smith, Jerome P.; Snawder, John C.; Striley, Cindy; MacKenzie, Barbara A.; Weissman, David N.

doi:10.1128/cdli.10.5.744-750.2003

Cited by 92 publications

(80 citation statements)

References 22 publications

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“…Additionally, similar studies have been carried out using samples derived from various animal models (Keyes et al, 2003(Keyes et al, , 2002Hutchinson et al, 2001). The multiplex analysis capability of the Luminex technology is being adapted to other assays including antibody screening (Biagini et al, 2004(Biagini et al, , 2003Jones et al, 2002;Opalka et al, 2003;Pickering et al, 2002a,b;Martins, 2002), HLA typing Osowski et al, 2003;Chesterton et al, 2003;Brailey and Susskind, 2003), single nucleotide polymorphism or mutation analysis (Hutchings et al, 2003;Kube et al, 2003;Smith et al, 1998;Yang et al, 2001;Ye et al, 2001), and pathogen detection (Jenison et al, 2001;Earley et al, 2002;Dunbar et al, 2003;Cowan et al, 2004). The flexibility of this technology along with its inherent advantages suggests that additional applications will be forthcoming.…”

Section: Discussionmentioning

confidence: 99%

“…This secondary antibody is either directly conjugated to the phycoerythrin (PE) fluorophore or biotin, which is then reacted with streptavidin-phycoerythrin. Although these technologies are gaining prominence and now have multiple commercial vendors for the analysis kits, there are relatively few reports of direct comparison between this technology and commercial ELISA kits (Camilla et al, 2001;Carson and Vignali, 1999;de Jager et al, 2003;Hildesheim et al, 2002;Hutchinson et al, 2001;Kellar et al, 2001;Oliver et al, 1998;Prabhakar et al, 2002;Vignali, 2000;Biagini et al, 2003). Only one of these reports utilizes commercially produced multiplex kits (Hildesheim et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

duPont¹,

Wang

Wadhwa

et al. 2005

Journal of Reproductive Immunology

274

181

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Problem: Analyses of the expression pattern of multiple cytokines are frequently required for characterization of the status of the immune system as it pertains to Th type bias and intrinsic levels of inflammation. Classically, analysis of cytokine expression patterns has been performed by enzymelinked immunosorbent assays (ELISA) for each separate analyte. A new technology, Luminex MAP ® , facilitates the simultaneous evaluation of multiple immune mediators with advantages of higher throughput, smaller sample volume, and lower cost. Validation of this technology has been limited to small sample sets, limited use of clinical study specimens, and use of non-commercial reagents. * Corresponding author. Methods: Ninety-six specimens from women over the course of their respective pregnancies were evaluated for cytokine concentrations using commercially available ELISA kits and commercially available Luminex MAP ® kits according to the manufacturers' directions. Correlations between data sets were evaluated using Pearson's correlation coefficient (r).Results: Excellent correlations were demonstrated for IL-1␤, IL-4, IL-5, IL-6, IL-10, IFN ␥, and TNF ␣, in contrast to IL-12 p70 and IL-13. Conclusions: Luminex multiplex technology has distinct advantages and is a valid alternative method to ELISA for the evaluation of the majority of cytokines tested and for the characterization of immune system status.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

duPont¹,

Wang

Wadhwa

et al. 2005

Journal of Reproductive Immunology

274

181

View full text Add to dashboard Cite

show abstract

“…In comparative testings, MIA shows a comparable sensitivity to ELISA but an improved dynamic range (Vignali, 2000;Biagini et al, 2003;DuPont et al, 2005). Moreover, MIA is less labour-intensive and different analytes can be measured at the same time (Dasso et al, 2002;Biagini et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, MIA is less labour-intensive and different analytes can be measured at the same time (Dasso et al, 2002;Biagini et al, 2003). Also when compared to antibody microarrays, the Luminex technology has better features, such as a lower detection limit and a better dynamic range, although microarrays are more suitable for miniaturization (Rao et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

An enrichment microsphere immunoassay for the detection of Pectobacterium atrosepticum and Dickeya dianthicola in potato tuber extracts

Peters¹,

Śledź

Bergervoet³

et al. 2006

Eur J Plant Pathol

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An enrichment microsphere immunoassay (MIA) was developed, based on the Luminex xMAPÒ technology, for the simultaneous (duplex) detection of Pectobacterium atrosepticum (former name Erwinia carotovora subsp. atroseptica) (Pca) and Dickeya dianthicola (former name Erwinia chrysanthemi) (Dcd) in potato plant extracts. Target bacteria in the extracts were enriched for 48 h in a semi-selective broth containing polypectate under low oxygen conditions. Samples were subsequently incubated with antibodycoated colour-coded microspheres (beads) and with secondary antibodies conjugated with Alexa FluorÒ 532, a reporter dye. Samples were analyzed with the Luminex analyzer, in which one laser identified each microsphere and another laser the reporter dye conjugated to the secondary antibodies. The assay required minimal sample preparation, could be completed in 1 h, was performed in 96 wells microtitreplates and required no wash steps.The limit of detection for the duplex enrichment MIA was 100-1000 cfu ml )1 , which was a hundred times lower than of an enrichment-ELISA. Without enrichment, the sensitivity of MIA and ELISA was largely similar and ranged between 10 6 and 10 7 cells ml )1 . No difference in sensitivity was found between a MIA in a single or duplex format. In a comparative test with non-infected potato plant extracts and extracts from plants infected with Pca or Dcd, results of the enrichment MIA correlated well with those of the enrichment ELISA and enrichment PCR. These results indicate that MIA can be reliably used for multiplex detection of soft rot Enterbacteriaceae in crude potato plant extracts. The technology is an attractive and cost-effective alternative to other detection methods, including ELISA.

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“…In the present study, we employed the microsphere as a solid phase to carry out the antigen-antibody reaction. The bead-based technique has been used in Multiplexed Fluorescent Microsphere Immunoassay [13] for the detection of various kinds of antigen and antibody, such as bacteria toxin [14], autoantigens in the serum of the patients [15] and specific antibodies against viral antigens [16]. The nature of the beads makes it possible to carry out immunoassay in a test tube and normal methods, such as stirring and votexing, can also be applied in both antibody immobilisation and antibody/antigen reaction.…”

Section: Discussionmentioning

confidence: 99%

Immuno-fluorescence detection of snake venom by using single bead as the assay platform

Gao¹,

Zhang²,

Gopalakrishnakone³

2008

Journal of Experimental Nanoscience

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By using micro-scale polystyrene beads as a platform, a rapid and sensitive method for the detection of snake venom was established. In the method, antivenom antibody or BSA was covalently fixed onto the microsbead to form the capture-bead or control bead. In first step of the experiment, the venom binds to the capture-bead to form the complex through the antibody-antigen interaction. The Qdot conjugated second antibody was then added. The second antibody targeted the Qdot to the capture-bead/antigen complex and form Qdot-second antibody-antigen-capture-bead complex. This complex can be directly observed under UV-microscope. The system was applied to the testing of Naja kaouthia venom and the detection limit of this method was 5-10 ng/ml.

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Method for Simultaneous Measurement of Antibodies to 23 Pneumococcal Capsular Polysaccharides

Cited by 92 publications

References 22 publications

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: Determinations of a panel of nine cytokines in clinical sample culture supernatants

An enrichment microsphere immunoassay for the detection of Pectobacterium atrosepticum and Dickeya dianthicola in potato tuber extracts

Immuno-fluorescence detection of snake venom by using single bead as the assay platform

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