Method to evaluate the quality of herbal medicines based on the dynamic changes of chemical compounds and pharmacological activity

Peipei, Zhou; Yang, Xiaolin; Zhang, Chun‐feng; Yang, Zhonglin; Li, Fēi

doi:10.1002/jssc.201600316

Cited by 10 publications

(11 citation statements)

References 15 publications

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“…Three parameters of ultrasonication were optimized. Different solvent volumes (20, 40, 50, and 70 mL), extraction times (10,20,30,40, and 60 min), and solvent percentages (30, 50, 70, and 100%, v/v, methanolwater) were employed for single factor analysis. Supporting Information Figure S2 displayed the variation trend of the nine alkaloids when the three factors changed.…”

Section: Optimization Of Extraction Conditionsmentioning

confidence: 99%

A biochemometrics strategy combining quantitative determination, bioactivity evaluation and relationship analysis for identification of analgesic alkaloids of raw and vinegar‐processed Corydalis turtschaninovii

Tao

Jiang

Cai

2020

J of Separation Science

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A biochemometrics strategy combining quantitative determination, bioactivity evaluation, and relationship analysis was proposed for identification of analgesic components of herbs. First, a robust liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of nine major alkaloids in crude and vinegar‐processed Corydalis turtschaninovii. Nine alkaloids were separated on a BEH C18 column with a mobile phase consisting of acetonitrile and water spiked with 0.1% formic acid and then detected by multiple reactions monitoring in the positive ion mode. Nitidine chloride was employed as the internal standard. The method displayed good linearity and the precisions of intra‐day and inter‐day were all within 3.0%. The recovery rates of each alkaloid ranged from 97.1 to 102.9%. The method was successfully applied for quantitative analysis of nine alkaloids in ten batches of crude and vinegar‐processed Corydalis turtschaninovii. Second, the analgesic effects of crude and vinegar‐processed Corydalis turtschaninovii were evaluated in mice. Third, principle component analysis, canonical correlation analysis, and partial least squares regression were used to analysis the relationship between the contents of nine major alkaloids and the analgesic effect of different crude and vinegar‐processed samples. Tetrahydropalmatine, coptisine, and dehydrocorydaline have a close positive correlation with the analgesic effect.

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Section: Optimization Of Extraction Conditionsmentioning

confidence: 99%

A biochemometrics strategy combining quantitative determination, bioactivity evaluation and relationship analysis for identification of analgesic alkaloids of raw and vinegar‐processed Corydalis turtschaninovii

Tao

Jiang

Cai

2020

J of Separation Science

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“…After being salt‐processed, the effects will be dramatically enhanced. Pharmacological researches revealed that the osteoblast proliferation activity of salt‐processed AB was significantly enhanced compared with that of raw sample .…”

Section: Introductionmentioning

confidence: 99%

“…Zhou et al. established a cell proliferation activity database and corresponding chemical fingerprints for 18 batches of AB salted samples . However, few endeavors were made to investigate the chemical alterations of AB components after processing, including β‐ecdysterone, 25 S ‐inokosterone, achyranthoside D, ginsenoside Ro, chikusetsusaponin IV, achyranthoside C, chikusetsaponin IVa and 13‐hydroxyoctadecadienoic acid (13‐HODE) .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Li et al developed an on-line coupled HPLC-DAD-ELSD method for quantification of four phytoecdysones and eight triterpenoids in four cultivated and ten commercial samples of AB [7]. Zhou et al established a cell proliferation activity database and corresponding chemical fingerprints for 18 batches of AB salted samples [4]. However, few endeavors were made to investigate the chemical alterations of AB components after processing, including β-ecdysterone, 25S-inokosterone, achyranthoside D, ginsenoside Ro, chikusetsusaponin IV, achyranthoside C, chikusetsaponin IVa and 13-hydroxyoctadecadienoic acid (13-HODE) [8].…”

Section: Introductionmentioning

confidence: 99%

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Integrated response surface methodology and UHPLC coupled with triple quadrupole time‐of‐flight MS quantitation to investigate the salt‐processing chemistry of traditional Chinese medicines: A case study on Achyranthes bidentata

Tao

Jia

et al. 2018

Separation Science Plus

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A sensitive ultra high performance liquid chromatography with tandem mass spectrometry approach for the quantification of eight bioactive constituents, including β‐ecdysterone, 25S‐inokosterone, achyranthoside D, ginsenoside Ro, chikusetsusaponin IV, achyranthoside C, chikusetsaponin IVa, and 13‐hydroxyoctadecadienoic acid, in raw and salt‐processed Achyranthes bidentata was developed and validated. Response surface methodology was employed for optimization of three parameters of ultrasound‐assisted extraction process, including the extraction time, methanol percentage, and solvent volume, to give optimum extraction conditions as follows: extraction time of 40.2 min, methanol percentage 67.2% v/v, and solvent volume of 48.4 mL. The established approach was interrogated in terms of linearity, sensitivity, precision, repeatability as well as recovery. Intra‐ and inter‐assay variability for all analytes ranged from 1.6 to 3.1% and from 2.0 to 3.2%, respectively. The standard addition method determined recovery rates for each analyte (99.2–103.6%). In addition, the developed approach was applied to 20 batches of collected raw samples of Achyranthes bidentata and their salt‐processed products. After salt‐processing, the contents of β‐ecdysterone, 25S‐inokosterone, achyranthoside D, chikusetsusaponin IV and achyranthoside C were elevated, while the content of ginsenoside Ro was decreased. The results showed that experimental design in combination with ultra high performance liquid chromatography with tandem mass spectrometry quantification provided insights into the chemistry alteration between raw Achyranthes bidentata and its salt‐processed product.

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“…Among several cell membrane sources for CMC, osteoblasts are the most ideal materials for screening components from TCMs because of the maximum simulation of in vivo osteogenesis effect . Osteoblasts are fundamentally characterized by their primary and exclusive function: the ability to form osseous tissue identifiable as bone .…”

Section: Introductionmentioning

confidence: 99%

Osteoblast cell membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry for screening specific active components from traditional Chinese medicines

et al. 2017

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A method using osteoblast membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry was developed to recognize and identify the specific active components from traditional Chinese medicines. Primary rat osteoblasts were used for the preparation of the stationary phase in the cell chromatography method. Retention components from the cell chromatography were collected and analyzed by liquid chromatography with time-of-flight mass spectrometry. This method was applied in screening active components from extracts of four traditional Chinese medicines. In total, 24 potentially active components with different structures were retained by osteoblast cell chromatography. There were five phenolic glucosides and one triterpenoid saponin from Curculigo orchioides Gaertn, two organic acids and ten flavonoids from Epimedium sagittatum Maxim, one phthalide compound and one organic acid from Angelica sinensis Diels, and two flavonoids and two saponins from Anemarrhena asphodeloides Bunge. Among those, four components (icariin, curculigoside, ferulaic acid, and timosaponin BII) were used for in vitro pharmacodynamics validation. They significantly increased the osteoblast proliferation, alkaline phosphatase activity, levels of bone gla protein and collagen type 1, and promoted mineralized nodule formation. The developed method was an effective screening method for finding active components from complex medicines that act on bone diseases.

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Method to evaluate the quality of herbal medicines based on the dynamic changes of chemical compounds and pharmacological activity

Cited by 10 publications

References 15 publications

A biochemometrics strategy combining quantitative determination, bioactivity evaluation and relationship analysis for identification of analgesic alkaloids of raw and vinegar‐processed Corydalis turtschaninovii

A biochemometrics strategy combining quantitative determination, bioactivity evaluation and relationship analysis for identification of analgesic alkaloids of raw and vinegar‐processed Corydalis turtschaninovii

Integrated response surface methodology and UHPLC coupled with triple quadrupole time‐of‐flight MS quantitation to investigate the salt‐processing chemistry of traditional Chinese medicines: A case study on Achyranthes bidentata

Osteoblast cell membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry for screening specific active components from traditional Chinese medicines

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