Method to obtain C-phycocyanin of high purity

Patil, Ganapathi; Chethana, S.; Sridevi, A.; Raghavarao, K.S.M.S.

doi:10.1016/j.chroma.2006.05.073

Cited by 188 publications

(101 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, optimization of parameters is necessary to extract the maximum amount. In microalgae research, ATPE is commonly used to extract phycobiliproteins from cyanobacteria like Spirulina (Patil et al, 2006;Patil & Raghavarao, 2007). However, to date, there are no studies that investigated the use of ATPE for eukaryotic microalgae protein extraction or from Nannochloropsis.…”

Section: Extraction Of Proteins From Nannochloropsismentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

134

View full text Add to dashboard Cite

Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

show abstract

Section: Extraction Of Proteins From Nannochloropsismentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

134

View full text Add to dashboard Cite

show abstract

“…The sample was purified from a preparation of Crespi [28,29] with nearly 99 % deuteration. The absorbance ratio A 620 nm /A 280 nm ≃ 7.3 is above the analytic-grade value of 5 [30]. The integrity of the secondary structure was ascertained by CD spectroscopy.…”

mentioning

confidence: 99%

Dynamical Transition of Protein-Hydration Water

et al. 2010

View full text Add to dashboard Cite

Thin layers of water on biomolecular and other nanostructured surfaces can be supercooled to temperatures not accessible with bulk water. Chen et al. [PNAS 103, 9012 (2006)] suggested that anomalies near 220 K observed by quasi-elastic neutron scattering can be explained by a hidden critical point of bulk water. Based on more sensitive measurements of water on perdeuterated phycocyanin, using the new neutron backscattering spectrometer SPHERES, and an improved data analysis, we present results that show no sign of such a fragile-to-strong transition. The inflection of the elastic intensity at 220 K has a dynamic origin that is compatible with a calorimetric glass transition at 170 K. The temperature dependence of the relaxation times is highly sensitive to data evaluation; it can be brought into perfect agreement with the results of other techniques, without any anomaly. In contrast to bulk water, protein hydration water can be supercooled down to a glass transition at T g ≃ 170 K. Near T g translational degrees of freedom arrest, which induces discontinuities in the specific heat and the thermal expansion coefficient of the hydration water [1][2][3][4]. Due to the dynamic nature of the glass transition, freezing of microscopic degrees of freedom can already be observed far above T g .The protein dynamic transition is an abrupt onset of atomic displacements on the microscopic length and time scale probed by quasielastic neutron scattering (QENS). First observed twenty years ago in hydrated myoglobin and lysozyme at T ∆ ≃ 240 K [5], it is now known to be a generic property of hydrated proteins, while it is absent in dehydrated systems. It is therefore related to the dynamics of the hydration shell.In QENS, mean squared dispacements δx 2 are deduced from the elastic scattering intensity S(q, 0). Due to the finite spectrometer resolution (fwhm 2∆ω), one actually measures S(q, |ω| ∆ω). Full spectral measurements show that the anomalous decrease of the central peak is compensated by increasing inelastic wings [5,6]. These effects have been interpreted as precursors of the glass transition. Since QENS probes structural relaxation at ∆ω −1 ≃ 100 ps, while the calorimetric T g refers to a time scale of 100 s, it is natural that T ∆ is located far above T g : the protein dynamic transition is the microscopic manifestation of the glass transition in the hydration shell. The time-scale dependence of T ∆ also explains its variation with viscosity [7][8][9]. * Electronic address: wdoster@ph.tum.de Recently, these views have been challenged by the suggestion that there might be a time-scale independent transition. Support came mainly from QENS experiments on the backscattering spectrometer HFBS at NIST. In hydrated lysozyme, DNA and RNA, a kink was found not only in S(q, |ω| ∆ω), but also in the α relaxation time τ deduced from full spectra S(q, ω) [10][11][12][13]. This change of τ (T ) from high-T super-Arrhenius to low-T Arrhenius behavior at T L ≃ 220 K has been interpreted as a fragile-to-strong-transition (FST) from the...

show abstract

“…3). Phycocyanin is made up of α and β subunits and the light absorption peak is around at 620 nm (Patil et al 2006), and the molecular weight of α subunit is around 17.0 kDa, whereas the molecular weight of β subunit is around 19.5 kDa (Thangam et al 2013). The size of the extracted protein (around 17 kD) and the absorbance peak (around 620 nm) indicated that the blue-colored protein is likely to be phycocyanin (Patel et al 2005).…”

Section: Characterization Of Released Proteins By Tricine-sds-page Anmentioning

confidence: 99%

Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin

Bai

Dai

Xia

et al. 2014

Environ Sci Pollut Res

View full text Add to dashboard Cite

Toxic Microcystis species are the main bloomforming cyanobacteria in freshwaters. It is imperative to develop efficient techniques to control these notorious harmful algal blooms (HABs). Here, we present a simple, efficient, and environmentally safe algicidal way to control Microcystis blooms, by using intermediate carboxylic acids from the tricarboxylic acid (TCA) cycle. The citric acid, alphaketoglutaric acid, succinic acid, fumaric acid, and malic acid all exhibited strong algicidal effects, and particularly succinic acid could cause the rapid lysis of Microcystis in a few hours. It is revealed that the Microcystis-lysing activity of succinic acid and other carboxylic acids was due to their strong acidic activity. Interestingly, the acid-lysed Microcystis cells released large amounts of phycocyanin, about 27-fold higher than those of the control. On the other hand, the transcription of mcyA and mcyD of the microcystin biosynthesis operon was not upregulated by addition of alpha-ketoglutaric acid and other carboxylic acids. Consider the environmental safety of intermediate carboxylic acids. We propose that administration of TCA cycle organic acids may not only provide an algicidal method with high efficiency and environmental safety but also serve as an applicable way to produce and extract phycocyanin from cyanobacterial biomass.

show abstract

Method to obtain C-phycocyanin of high purity

Cited by 188 publications

References 19 publications

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Dynamical Transition of Protein-Hydration Water

Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin

Contact Info

Product

Resources

About