Method validation for the analysis of pesticide residue in aqueous environment

Koçyiğit, Hasan; Sinanoğlu, Firdevs

doi:10.1007/s10661-020-08523-8

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…With the development of agriculture, the spraying of various pesticides has a significant impact on the living environment of insects. Pesticide residues exist in farmland, water, atmosphere and plants ( Koçyiğit & Sinanoğlu, 2020 ). A large number of pesticide residues require that insects have the ability to resist pesticides.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

Liu

2023

PeerJ

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Formosatettix qinlingensis (Zheng, 1982) is a tiny grasshopper endemic to Qinling in China. For further study of its transcriptomic features, we obtained RNA-Seq data by Illumina HiSeq X Ten sequencing platform. Firstly, transcriptomic analysis showed that transcriptome read numbers of two female and one male samples were 25,043,314, 24,429,905, and 25,034,457, respectively. We assembled 65,977 unigenes, their average length was 1,072.09 bp, and the length of N50 was 2,031 bp. The average lengths of F. qinlingensis female and male unigenes were 911.30 bp, and 941.82 bp, and the N50 lengths were 1,745 bp and 1,735 bp, respectively. Eight databases were used to annotate the functions of unigenes, and 23,268 functional unigenes were obtained. Besides, we also studied the body color, immunity and insecticide resistance of F. qinlingensis. Thirty-nine pigment-related genes were annotated. Some immunity genes and signaling pathways were found, such as JAK-STAT and Toll-LIKE receptor signaling pathways. There are also some insecticide resistance genes and signal pathways, like nAChR, GST and DDT. Further, some of these genes were differentially expressed in female and male samples, including pigment, immunity and insecticide resistance. The transcriptomic study of F. qinlingensis will provide data reference for gene prediction and molecular expression study of other Tetrigidae species in the future. Differential genetic screening of males and females provides a basis for studying sex and immune balance in insects.

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Section: Introductionmentioning

confidence: 99%

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

Liu

2023

PeerJ

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“…By examining matrix-matched calibration curves with spiked blank samples at concentrations of 0.01 0.05, 0.1, 0.25, and 1 mg/L, the linearity was proven. Standard deviations of response and slope were used to calculate LODs and LOQs, which are represented as follows [19]:…”

Section: Methods Validationmentioning

confidence: 99%

“…where S stands for ten times the blank analysis's standard deviation [19]. Furthermore, matrix-matched standards were applied to take into account the matrix effects (MEs), as stated in SANTE/12682/2019 [15].…”

Section: Methods Validationmentioning

confidence: 99%

A Dynamic Multiple Reaction Monitoring Analytical Method for the Determination of Fungicide Residues in Drinking Water

Arvanitidis,

Adamidis,

Parlakidis

et al. 2023

Environments

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The extensive use of fungicides causes their continuous release into the environment through spraying, soil seepage, leaching, and runoff. It has been observed that their residues can be found in foods and a variety of environmental compartments, such as wastewater, lakes, rivers, sediments, drinking water sources (groundwater and surface water), treated water, and drinking water. A sensitive GC-MS/MS, using dynamic multiple reaction monitoring, an analytical method was developed to determine 10 fungicides (azoxystrobin, boscalid, captan, cyproconazole, cyprodinil, hexaconazole, metalaxyl, myclobutanil, paclobutrazol, and prochloraz) in drinking water. A solid-phase extraction method for sample preparations and validations was performed according to SANTE 2019 guidelines. All fungicides demonstrated mild or medium matrix effects (ME) ranging from 40.1% to 11.2%. Their recoveries ranged between 60% and 110%. The limits of detection were equal to or higher than 0.01 μg/L. The method was employed on 18 drinking water samples collected from public taps in Northern Evros, Greece, distributed in six sampling sites. Azoxystrobin, boscalid, cyproconazole, cypronidil, metalaxyl, and paclobutrazol mean concentrations did not surpass the allowable limit of 0.1 μg/L set by EU in any sampling site. Hexaconazole mean concentrations were higher than 0.1 μg/L in one sampling site, while prochloraz mean concentration showed limit exceedances in all sampling sites. Captan was not detected in any sampling site, and myclobutanil mean concentrations demonstrated exceedances of the permissible limit in four sampling sites. The presence of fungicide residues in the studied area is mainly due to the occasional point-sources pollution and preferential flow. Additionally, through the use of water, the risk of pesticides to human health was assessed for two different age groups. The sum of the hazard quotient values in each of the studied drinking water was less than unity. Consequently, the acute risk assessment procedure regards the examined drinking water as safe. Nevertheless, as prochloraz carcinogenic risk values were higher than the safe limit suggested by USEPA for both age groups, the existence of prochloraz residues raises concerns about chronic toxicity.

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“…On the other hand, indirect or non-occupational exposure includes the ingestion of pesticide residues in contaminated food and water, as well as the inhalation of pesticide droplets present in the air [29]. Although in most cases the concentrations present in food do not exceed the safety limits determined by legislation, in several regions around the world the pesticide residues detected in food or in water clearly exceed the permitted limits [30][31][32]. Furthermore, it should also be considered that the safety limits established for individual pesticides may underestimate the true risk to health, since in real life people are chronically exposed to pesticide mixtures, the toxic effects of which may be independent, additive, or synergistic [33].…”

Section: Introductionmentioning

confidence: 99%

Systematic Review of Calcium Channels and Intracellular Calcium Signaling: Relevance to Pesticide Neurotoxicity

Costas-Ferreira

Faro

2021

IJMS

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Pesticides of different chemical classes exert their toxic effects on the nervous system by acting on the different regulatory mechanisms of calcium (Ca2+) homeostasis. Pesticides have been shown to alter Ca2+ homeostasis, mainly by increasing its intracellular concentration above physiological levels. The pesticide-induced Ca2+ overload occurs through two main mechanisms: the entry of Ca2+ from the extracellular medium through the different types of Ca2+ channels present in the plasma membrane or its release into the cytoplasm from intracellular stocks, mainly from the endoplasmic reticulum. It has also been observed that intracellular increases in the Ca2+ concentrations are maintained over time, because pesticides inhibit the enzymes involved in reducing its levels. Thus, the alteration of Ca2+ levels can lead to the activation of various signaling pathways that generate oxidative stress, neuroinflammation and, finally, neuronal death. In this review, we also discuss some proposed strategies to counteract the detrimental effects of pesticides on Ca2+ homeostasis.

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Method validation for the analysis of pesticide residue in aqueous environment

Cited by 13 publications

References 28 publications

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

A Dynamic Multiple Reaction Monitoring Analytical Method for the Determination of Fungicide Residues in Drinking Water

Systematic Review of Calcium Channels and Intracellular Calcium Signaling: Relevance to Pesticide Neurotoxicity

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