Method validation of analytical method for 12 flavonol glycosides in foods using ultra high-performance liquid chromatography coupled with photodiode array detection

Abstract

An analytical method for the simultaneous determination of 12 flavonol glycosides in buckwheat, black tea, and wild parsley using ultra high-performance liquid chromatography (UHPLC) coupled with a simple liquid extraction method using dimethyl sulfoxide (DMSO) was validated in precision, accuracy, and linearity. The UHPLC separation of target compounds was performed on a C18 column using a photodiode array (PDA) detector and the wavelength was fixed at 350 nm. The recovery values for flavonol glycosides ranged from 85.44 to 108.79%. The limits of detection and limits of quantification were less than 0.32 mg/kg and less than 0.97 mg/kg, respectively. The intraday and interday precisions were less than 13.69% for all the test samples. This method coupled with UHPLCPDA detection could be expected to provide more convenient sample preparation than conventional methods in the tested foods.

Optimization of Flavonoid Production in Plant Cell Culture of Thevetia peruviana Elicited with Methyl Jasmonate and Salicylic Acid

“…These compounds are produced by acetate/malonate, shikimic acid, pentose phosphate metabolic pathway [10], and through metabolism of phenylpropanoids [11]. The flavonols are the most promising group in flavonoids due to their antimicrobial and anti-carcinogenic properties, and for its potential in the treatment of cardiovascular diseases [12].…”