Méthode d'ANALYSE Quantitative De La Spermatogenèse Des Poissons Téléostéens

Billard, R.; Solari, Aline; Escaffre, Anne-Marie

doi:10.1051/rnd:19740108

Cited by 7 publications

(4 citation statements)

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“…Des coupes de 3 A 5 tm sont effectuees et les lames microscopiques preparees a partir de chaque echantillon sont colorees par un trichrome a chaud a l'hematoxyline de Regaud. La determination, le comptage et l'estimation du volume des different types cellulaires se font grace A un oculaire integrateur de type I (Zeiss) suivant la methode des numerations ponctuelles (Billard et al 1974). L'examen histologique permet en outre d'identifier des dgenerescences testiculaires qui affectent les cellules germinales (cfr photo 4).…”

Section: Materiel Et Methodesunclassified

Etude de la reproduction du gardon (Rutilus rutilus) et des effets d'une élévation provoquée de la température en Meuse sur cette reproduction

et al. 1981

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The thermal pollution of the Tihange Nuclear Power Plant increases the temperature (± 3 C) of the river Meuse and has precise effects on the reproductive cycle of the roach (Rutilus rutilus) downstream: welldefined, ovarial development, acceleration of maturation, and earlier laying of eggs (3 weeks). The volume of the different testicular cell types appears to be more important in males downstream. Spermatozoa are retained for a longer period in both populations (upstream and downstream) as a result of diminished spawning-grounds along the river.

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Section: Materiel Et Methodesunclassified

Etude de la reproduction du gardon (Rutilus rutilus) et des effets d'une élévation provoquée de la température en Meuse sur cette reproduction

et al. 1981

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“…Briefly, we excised male gonads, fixed them in Bouin’s solution, and stained 7 µm-thick tissue sections with hematoxylin-eosin (Diapath) followed by mounting with afcolene mounting medium. We determined the stage of gonadal maturation by macroscopic (whole gonads) and histological (gonad sections) analyses, according to (71). As DMC1 binds to chromatin during the early stages of the meiotic prophase I, we used testes at stages III and IV from three individuals with different Prdm9 genotypes (TAC-1: Prdm9 1/5 , stage IIIb; TAC-3: Prdm9 2/6 , stage IIIa; and RT-52: Prdm9 1/2 , stage IV).…”

Section: Methodsmentioning

confidence: 99%

PRDM9 drives the location and rapid evolution of recombination hotspots in salmonids

Raynaud,

Sanna,

Joseph

et al. 2024

Preprint

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In many eukaryotes, meiotic recombination occurs preferentially at discrete sites, called recombination hotspots. In various lineages, recombination hotspots are located in regions with promoter-like features and are evolutionarily stable. Conversely, in some mammals, hotspots are driven by PRDM9 that targets recombination away from promoters. Paradoxically, PRDM9 induces the self-destruction of its targets and this triggers an ultra-fast evolution of mammalian hotspots. PRDM9 is ancestral to all animals, suggesting a critical importance for the meiotic program, but has been lost in many lineages with surprisingly little effect on meiosis success. However, it is unclear whether the function of PRDM9 described in mammals is shared by other species. To investigate this, we analyzed the recombination landscape of several salmonids, the genome of which harbors one full-length PRDM9 and several truncated paralogs. We identified recombination initiation sites in Oncorhynchus mykiss by mapping meiotic DNA double-strand breaks (DSBs). We found that DNA DSBs clustered at hotspots positioned away from promoters, enriched for the H3K4me3 and H3K4me36 marks and the location of which depended on the genotype of full-length Prdm9. We observed a high level of polymorphism in the zinc finger domain of full-length Prdm9, but not of the truncated paralogs. Moreover, population-scaled recombination maps in O. mykiss, Oncorhynchus kisutch and Salmo salar revealed a rapid turnover of recombination hotspots caused by PRDM9 target motif erosion. Our results imply that PRDM9 function is conserved across vertebrates and that the peculiar evolutionary runaway caused by PRDM9 has been active for several hundred million years.

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“…Little quantitative work has been done on teleost reproduction, with the exception of estimates of fecundity which are limited to the breeding season. The reproductive stage or potential of fish can be assessed by stereological methods (Steer, 1981) at any season by the use of a score based on the volume fraction of each germ cell type determined by points counting methods (Billard et al, 1974;Billard, 1983).…”

Section: Introductionmentioning

confidence: 99%

Quantitative histology of the seasonal changes in the gonads of summer whiting, Sillago ciliata

Goodall

Capra

Blackshaw

1987

Journal of Fish Biology

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The histology of the gonads of free and captive summer whiting, Sillugo ciliara. were compared to assess the potential of this fish for aquaculture. There were no qualitative differences between the free and captive males: both completed spermatogenesis through to spermiation. The captive females were non-reproductive, reaching only the vitellogenic stage before the eggs were resorbed by atresia.A reproductive score was devised to assess reproductive potential in the comparison of the two populations. The females in the captive population had lower reproductive potentials. as shown by the score, and started development a month later than the normal free population. Thecaptive males had higher reproductive scores than the free fish but they were also not spawning and, therefore, the higher score indicated that this potential was not realized. The score provides a standard against which experimental results can be compared and subsequently assessed.

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Méthode d'ANALYSE Quantitative De La Spermatogenèse Des Poissons Téléostéens

Abstract: RÉSUMÉ

Cited by 7 publications

References 0 publications

Etude de la reproduction du gardon (Rutilus rutilus) et des effets d'une élévation provoquée de la température en Meuse sur cette reproduction

Etude de la reproduction du gardon (Rutilus rutilus) et des effets d'une élévation provoquée de la température en Meuse sur cette reproduction

PRDM9 drives the location and rapid evolution of recombination hotspots in salmonids

Quantitative histology of the seasonal changes in the gonads of summer whiting, Sillago ciliata

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