Soils enriched with biochar are recommended as a cultivation grounds, especially in case they contain significant amount of sand. However, the interactions between biochar and plants, as well as the influence of the biochar on water retention, cultivation and air properties of soils, are still not obvious. The present study aimed to determine the impact of various biochar doses on soils used for soya cultivation, in comparison to soils maintained as black fallow soil, on their water retention and productivity, for the period of two years. Sunflower husk biochar (BC1) and biochar of leafy trees (BC2), in doses of 0, 40, 60, 80 t·ha−1, were used for field experiments. The water retention was investigated with porous boards in pressure chambers by a drying method. No differences in the hydrological properties of the soils that were differently managed (black fallow soil, crop) were observed following biochar application. Addition of BC1, in the amounts of 40, 60, and 80 t·ha−1, caused an increase in the plant available water capacity (AWC) by 15.3%, 18.7%, and 13.3%, respectively, whereas the field capacity (FC) increased by 7.4%, 9.4%, and 8.6% for soils without biochar. Application of BC2 analogously resulted in higher AWC, by 8.97, 17.2%, and 33.1%, respectively, and higher FC by 3.75, 7.5%, and 18.3%, respectively. Increasing the doses of BC1 and BC2, both on black fallow soils and soils enriched with soya, caused a rise in total porosity (TP) and drainage porosity (DP), and a decrease in soil bulk density (SBD). Biochar with a higher total area and higher porosity (BC1) applied to soils with soya cultivation resulted in lower reductions in AW and FC than BC2 in the second year of investigation.