Methodical peculiarities and guidelines for the determination of chemical compounds and elements in the biological matrices

Ulanova, T.S.; Nurislamova, Tatyana V.; Karnazhitskaya, T.D.; Gileva, Olga

doi:10.18821/0016-9900-2016-95-1-112-116

Cited by 6 publications

(3 citation statements)

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“…Метод LUR существенно улучшает результаты оценки рассеивания примесей при более тщательном выборе и описании места проведения натурных измерений [14]. [16].…”

Section: обозначим наиболее вероятные варианты взаимной координации оunclassified

Spatial factor of damages from urban air pollution

Krupina¹

2018

NIPS

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Section: обозначим наиболее вероятные варианты взаимной координации оunclassified

Spatial factor of damages from urban air pollution

Krupina¹

2018

NIPS

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“…В 2017 году [9] опубликованы исследования референтных значений RV95 таких элементов, как As, Cd, Co, Cu, Pb, Mn, Hg, Mo, Ni, Se, Zn, в крови населения Канады различного возраста. В Российской Федерации биомониторинг проводят путем сравнения содержания химических элементов в биологических средах экспонированных групп с фоновыми значениями для соответствующих регионов [10,11] или с данными по клиническим и лабораторным тестам [12,13]. Совершенствование и развитие биомониторинга с расширением спектра определяемых специфических биомаркёров приведет к повышению объективности оценки риска воздействия на здоровье населения в результате загрязнения окружающей среды.…”

Section: Introductionunclassified

Application inductively coupled plasma mass spectrometry for assessing the content of essential and toxic elements in whole blood of unexposed adults in the Russian Federation

Zaitseva

Ulanova²,

Nedoshitova³

et al. 2022

Ekologiya cheloveka (Human Ecology)

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Introduction: Determination of essential and toxic elements in human blood is an important and urgent task within monitoring, environmental and hygienic studies accomplished in evidence-based medicine when population health risks are assessed. Aim: Biomonitoring of unexposed adult population in the Russian Federation. Methods: The contents of V, Cr, Mn, Ni, Cu, Zn, As, Se, Sr, Cd, Tl and Pb were determined in the blood of adults (n=80, aged 40-45 years) living in rural areas in the Western Urals region of the Russian Federation. The measurements were carried out using Agilent 7500cx quadrupole inductively coupled plasma mass spectrometer (Agilent Technologies, USA) with an octopole reaction-collision cell (ORS) in conformity with the Methodical Guidelines 4.1.3230-14 and 4.1.3161-14 (FR. 1.31.2014.17064) developed by the authors. Results: The arithmetic mean (AM) content of elements in blood of this group amounted to 0.13 g/l (V); 4.75 g/l (Cr); 13.41 g/l (Mn); 4.06 g/l (Ni); 827.15 g/l (Cu); 5369.36 g/l (Zn); 0.42 g/l (As); 123.10 g/l (Se); 22.75 g/l (Sr); 0.45 g/l (Cd); 0.04 g/l (Tl); 14.37 g/l (Pb). The validity of the results was confirmed by analyzing standard samples of SERONORMTM Whole Blood L1 and SERONORMTM Whole Blood L2 (Norway). The results are presented as basic statistical indicators: minimum and maximum values, arithmetic mean (AM), 5th, 50th, 95th percentiles and are interpreted in accordance with up-to-date international requirements. Conclusions: We compared the range of P5-P95 values for unexposed groups of adults in the Western Urals and the Far North region with the results produced by several hygienic studies conducted in Germany, Italy, France and Canada. As a result, we established elevated concentrations of chromium, manganese, and nickel in the blood of adults from Russia. The contents of copper, zinc, selenium, and arsenic in the blood of adults living in the Russian Federation were different from the reference values used in such diagnostic laboratories as ALS Scandinavia, SIVR LIST Italy and in the monograph by Norbert Titz (USA).

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“…In the RF, research results are interpreted by comparing elemental content in biological media of exposed groups against regional background values [11,12] or data taken from guides on clinical and laboratory tests [13,14].…”

Section: Introductionmentioning

confidence: 99%

Biomonitoring of 12 Trace Elements in Blood and Urine of Children by Inductively Coupled Plasma Mass Spectrometry<strong> </strong>

Veikhman¹,

Зайцева²,

Ulanova³

et al. 2019

Preprint

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The authors determined mass concentrations of 12 elements (As, Cd, Cr, Cu, Mn, Ni, Pb, Se, Sr, Tl, V, Zn) in blood and urine taken from 100 rural children living in unremarkable condition in the West Ural region of the Russian Federation. We applied inductively coupled plasma mass spectrometry in conformity with Methodical Guidelines 4.1. 3230-14 (FR.1.31.2014.17064) designed by us and detailed in the paper. The article contains setting parameters for Agilent 7500cx quadrupole inductively coupled plasma mass spectrometer . The authors present grounds for an optimal scheme of samples preparation which helps to eliminate "matrix" effect and explain why it is advisable to use reaction/collision functioning mode as it enables interference overlaps suppression.To prepare whole blood samples for analysis, we used acid dissolution in concentrated nitric acid followed by centrifugation. The arithmetic mean (AM) of element content in blood amounted to 0.34 µg/l (As); 0.39 µg/l (Cd); 6.41 µg/l (Cr); 866.11 µg/l (Сu); 13.73 µg/l (Mn); 5.86 µg/l (Ni); 23.65 µg/l (Pb); 87.43 µg/l (Se); 33.54 µg/l (Sr); 0.04 µg/l (Tl); 0.16 µg/l (V); 4713.48 µg/l (Zn). The validity of the results was confirmed by means of  SERONORM TM Whole Blood L2 standard samples (Norway). Urine samples were directly analyzed after 1/10 (V/V) dilution with 1% nitric acid solution. The arithmetic mean of element  content of urine corresponded to 18.99 µg/l (As); 0.12 µg/l (Cd); 1.91 µg/l (Cr); 13.28 µg/l (Сu);  0.96 µg/l (Mn); 1.84 µg/l (Ni); 0.83 µg/l  (Pb); 22.55 µg/l (Se); 239.09 µg/l (Sr); 0.16 µg/l  (Tl); 0.68 µg/l (V); 270.56 µg/l (Zn).  The validity of the results was confirmed by analyzing the SERONORM TM standard urine samples (Norway). Regional differences from levels found in other countries included  higher concentrations of Cr, Mn and Ni in blood and  lower levels of Se and Zn levels in West Ural children. Urine concentrations of West Ural children showed  higher levels of As and Sr.

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Methodical peculiarities and guidelines for the determination of chemical compounds and elements in the biological matrices

Cited by 6 publications

References 0 publications

Spatial factor of damages from urban air pollution

Spatial factor of damages from urban air pollution

Application inductively coupled plasma mass spectrometry for assessing the content of essential and toxic elements in whole blood of unexposed adults in the Russian Federation

Biomonitoring of 12 Trace Elements in Blood and Urine of Children by Inductively Coupled Plasma Mass Spectrometry<strong> </strong>

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