Methodologic Prerequisites for a Differential Diagnosis of Lymphomas

Lennert, K.

doi:10.1007/978-3-642-81092-3_2

Cited by 8 publications

(4 citation statements)

References 513 publications

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“…Histological sections of all organs were stained with hematoxylin-eosin (H & E). Additional stains were applied to mesenteries and omenta, such as Lennert's Giemsa (Lennert 1978) and Gomori's reticulin stains for brightfield microscopy and phosphomolybdic acid-picrosirius red (PMA-PSR) (collagen) (Dolber & Spach 1993), Sirius red pH 10.2 (eosinophils) (Bogomoletz 1980, Luque & Montes 1989, Vale et al 1997), Masson's trichrome (plasma cells), and Alcian blue/safranin (mast cell subpopulation) (Strobel et al 1981) for confocal laser scanning microscopy (CLSM). In 15 animals with 55, 90 and 160 days of infection, the mesenteries were distended using an intraintestinal spiral copper wire and also fixed in Carson's formalin-Millonig and stained with Lennert's Giemsa for 2 hr.…”

Section: Methodsmentioning

confidence: 99%

Effects of Schistosomal mansoni infection on Calomys callosus coelom-associated lymphomyeloid tissue (milky spots)

Lenzi¹,

Pelajo-Machado²,

Mota³

et al. 1998

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Effects of Schistosomal mansoni infection on Calomys callosus coelom-associated lymphomyeloid tissue (milky spots)

Lenzi¹,

Pelajo-Machado²,

Mota³

et al. 1998

Mem. Inst. Oswaldo Cruz

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“…Complete >-ixm sections were cut from these blocks to ohtain a compleic profile of the organ. The sections were siained with haematoxylin and eosin: with Gienisa modified by Lenncn [25); with silver impregnation aeet)rding to Biclschowsky modified in Gomori [27]. Biopsy malerial was treated identically, except for the determination of weighi and volume.…”

Section: Methodsmentioning

confidence: 99%

The Involution of the Ageing Human Thymic Epithelium is Independent of Puberty

1985

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One hundred and thirty-six thymuses completely removed at autopsy from persons suffering a sudden death were examined by stereological and morphometrical methods. Adding biopsy material from immunologically healthy cardiac patients we obtained relative volumes from 204 persons ranging in age from 1 month to 107 years. The size of the human thymus remains unchanged during ageing under normal physiological conditions (median: 19.5 cm3). Individual maximum size (range: 5-70 cm3) is reached in the first year of life. Early histological changes are in the enlargement of the perivascular space, the Hassall's bodies, and the connective tissue. This begins in the first year of life, reaches a maximum from 10 to 25 years, then declines again. Adipose tissue replaces the lymphocytic perivascular space and the connective tissue only. This occurs extensively after the age of 15 years. When defined by the silver impregnation technique, the volumes of the thymic epithelium (cortex and medulla), show a continuous involution from the first year to the end of life. The curve can be approximated to simple negative logarithmic functions. The velocity and nature of involution of the thymic epithelium do not change under the influence of the changing hormonal balance due to puberty. Since important thymic functions (T lymphopoiesis and T-cell differentiation) are located in the epithelial space, the age-related involution of the human thymus is not related to puberty.

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“…The perivascular space, which was quantitatively determined by using the silver impregnation technique of GOMORI (1937) [for method see LENNERT (1978)], showed a course of growth and involution similar to that of connective tissue and Hassall's corpuscles (Fig. 11).…”

Section: Morphology Of the Aging Human Thymusmentioning

confidence: 99%