Methodological advances and challenges in probiotic bacteria production: Ongoing strategies and future perspectives

Hathi, Zubeen; Mettu, Srinivas; Priya, Anshu; Athukoralalage, Sandya; Lam, Tsz Nok; Choudhury, Namita Roy; Dutta, Naba K.; El‐Omar, Emad; Gong, Lan; Mohan, Geethaanjali; Lin, Carol Sze Ki

doi:10.1016/j.bej.2021.108199

Cited by 16 publications

(12 citation statements)

References 123 publications

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“…Microbial growth is controlled by cell density measurements and light/fluorescence microscopes are used to check for unwanted contaminations. Once batch fermentation is complete, a filtered and concentrated cells suspension is either spray-dried or freeze-dried but previously, cryoprotectants or lyoprotectants are added to prevent loss of microbial viability (131). To increase the production rate the batch fermentation is integrated with crossflow membrane ultra/microfiltration; when the desired cell density is reached, toxic metabolites and/or acids are removed through a membrane.…”

Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

“…and Lactobacillus related genera are from 10 9 to 10 10 CFU, while for Bifidobacterium spp. at 10 8 –10 10 CFU, for Pediococcus acidilactici 10 9 CFU, for Streptococcus thermophilus 10 8 CFU, for yeast strains such as Saccharomyces boulardii 10 9 CFU, Bacillus subtilis 10 9 CFU and A. muciniphila 10 8 CFU ( 131 ). It is worth mentioning that the dose is also a function of storage conditions, as some preparations should be stored at room temperature, while others require refrigeration; therefore, a thermal abuse could heavily affect probiotic survival.…”

Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

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A narrative review on the use of probiotics in several diseases. Evidence and perspectives

et al. 2023

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Gut microbiota is a complex ecosystem, strictly linked to health and disease, as a balanced composition (referred as eubiosis) is necessary for several physiological functions, while an unbalanced composition (dysbiosis) is often associated to pathological conditions and/or diseases. An altered microbiota could be positively affected and partially restored through probiotic supplementation, among others. This review addresses the effects of probiotics in several conditions, used as case-studies (colorectal cancer, neuro-psychiatric diseases, intestinal diseases, obesity, diabetes, metabolic syndrome, immune system, and musculoskeletal system disorders) by pointing out the clinical outcomes, the mode of action, mainly related to the production of short chain fatty acids (SCFA), the impact of probiotic dose and mode of supplementation, as well as trying to highlight a hit of the most used genera.

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Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

Section: Probiotic Species Dose Delivery and Productionmentioning

confidence: 99%

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A narrative review on the use of probiotics in several diseases. Evidence and perspectives

et al. 2023

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“…At the end of the cultivation stage, the cells can be either used for direct consumption, as cell suspensions, or separated from the medium through filtration or centrifugation approaches, concentrated and subjected to drying processes (lyophilization or atomization) for later incorporation into food matrices or sale in the form of powder in capsules, sachets, among others. To avoid the loss of cell viability inherent to the drying processes, some protective agents can be added in advance [ 130 ].…”

Section: From the Physiology Of Production To The Processing Of The P...mentioning

confidence: 99%

Journey of the Probiotic Bacteria: Survival of the Fittest

et al. 2022

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This review aims to bring a more general view of the technological and biological challenges regarding production and use of probiotic bacteria in promoting human health. After a brief description of the current concepts, the challenges for the production at an industrial level are presented from the physiology of the central metabolism to the ability to face the main forms of stress in the industrial process. Once produced, these cells are processed to be commercialized in suspension or dried forms or added to food matrices. At this stage, the maintenance of cell viability and vitality is of paramount for the quality of the product. Powder products requires the development of strategies that ensure the integrity of components and cellular functions that allow complete recovery of cells at the time of consumption. Finally, once consumed, probiotic cells must face a very powerful set of physicochemical mechanisms within the body, which include enzymes, antibacterial molecules and sudden changes in pH. Understanding the action of these agents and the induction of cellular tolerance mechanisms is fundamental for the selection of increasingly efficient strains in order to survive from production to colonization of the intestinal tract and to promote the desired health benefits.

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“… Lacticaseibacillus rhamnosus has become one of the most widely used LAB species due to its advantages over other species ( 18 ). Lacticaseibacillus rhamnosus Probio-M9 was isolated from a breast milk sample from a healthy woman and has good probiotic potential, exhibiting a high growth rate and strong tolerance to bile salts and artificial gastrointestinal fluids ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

The Space Environment Activates Capsular Polysaccharide Production in Lacticaseibacillus rhamnosus Probio-M9 by Mutating the wze ( ywqD ) Gene

et al. 2023

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Methodological advances and challenges in probiotic bacteria production: Ongoing strategies and future perspectives

Cited by 16 publications

References 123 publications

A narrative review on the use of probiotics in several diseases. Evidence and perspectives

A narrative review on the use of probiotics in several diseases. Evidence and perspectives

Journey of the Probiotic Bacteria: Survival of the Fittest

The Space Environment Activates Capsular Polysaccharide Production in Lacticaseibacillus rhamnosus Probio-M9 by Mutating the wze ( ywqD ) Gene

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