Methodological advances and future directions of microalgal bioassays for evaluation of potential toxicity in environmental samples: A review

Lee, Jung-Hyun; Hong, Seongjin; An, Seong-Ah; Khim, Jong Seong

doi:10.1016/j.envint.2023.107869

Cited by 17 publications

(3 citation statements)

References 146 publications

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“…Accordingly, microalgae are considered a useful and crucial indicator to evaluate the deterioration of environmental quality (Lee et al, 2023). Thus, the current study applying microalgae assays to investigate the effects of Mg(OH)2 suggests a low negative biological impact of Mg(OH)2.…”

Section: Discussionmentioning

confidence: 90%

Biological impact of ocean alkalinity enhancement of magnesium hydroxide on marine microalgae using bioassays simulating ship-based dispersion

Delacroix,

Nystuen,

Höglund

et al. 2023

Preprint

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Abstract. Increasing the marine CO2 absorption capacity by adding alkaline minerals into the world’s oceans is a promising marine carbon dioxide removal (mCDR) approach to increase the ocean’s CO2 storage potential and mitigate ocean acidification. Still, the biological impacts of dispersion of alkaline minerals needs to be evaluated prior to its field deployment. In this study, the toxicity effect on marine microalgae of two commonly used alkaline minerals, calcium hydroxide (Ca(OH)2) and sodium hydroxide (NaOH), was compared with magnesium hydroxide (Mg(OH)2), by applying the same concentration of hydroxyl radicals (OH-) for each component. Cultures of marine green microalgae Tetraselmis suecica were exposed to NaOH, Ca(OH)2 or Mg(OH)2 in concentrations mimicking dispersion scenarios from a ship which included short-term exposure with high alkaline mineral concentration called “dispersion phase” followed by a dilution and “regrowth” phase over six days. There was no detectable effect of Mg(OH)2 treatment on algae growth either after the dispersion phase or during the regrowth phase, compared to control treatments. The Ca(OH)2 treatment resulted in very few living algal cells after the dispersion phase, but a similar growth rate was observed during the regrowth phase as was for the Mg(OH)2 and control treatments. The NaOH treatment resulted in no surviving algae after the dispersion phase and during the regrowth phase. Standardized whole effluent toxicity (WET) tests were carried out with a range of Mg(OH)2 concentrations using a sensitive marine diatom, Skeletonema costatum, which confirmed the relative low toxicity effect of Mg(OH)2. Similar biological effects were observed on natural microalgae assemblages from a local seawater source when applying the same Mg(OH)2 concentration range and exposure time used in the WET tests. The results suggest that Mg(OH)2 is relatively safe compared to Ca(OH)2 and NaOH with respect to marine microalgae.

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Section: Discussionmentioning

confidence: 90%

Biological impact of ocean alkalinity enhancement of magnesium hydroxide on marine microalgae using bioassays simulating ship-based dispersion

Delacroix,

Nystuen,

Höglund

et al. 2023

Preprint

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“…Model plants that have been used for air pollutant assessment include Arabidopsis, tobacco, wheat, rice, soybean, maize, and sorghum [237][238][239]. The plants evaluated for toxicity of aquatic organisms are mainly algae, such as chlorella and microalgae [240,241]. The selection of plant species is related to geographical factors, and researchers usually choose common plant species in the area to be studied.…”

Section: Biological Modelsmentioning

confidence: 99%

Zero-Carbon and Carbon-Neutral Fuels: A Review of Combustion Products and Cytotoxicity

Jin,

Li,

et al. 2023

Energies

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The use of zero-carbon and carbon-neutral fuels reduces emissions of conventional pollutants, but their emissions can be toxic and have various adverse effects on human health. This article reviews the possible combustion products of zero-carbon and carbon-neutral fuels, as well as their cytotoxic effects and potential health risks. At the same time, the review outlines biological models and toxicity detection methods commonly used in pollutant toxicity studies. Metals, nitrogen oxides (NOX), and ammonia (NH3) emitted from the combustion of metal fuels, hydrogen fuels, and ammonia fuels in zero-carbon fuels are harmful to human health. Exhaust emissions from carbon-neutral fuels, particularly biodiesel, and their blends with gasoline/diesel are cytotoxic, leading to severe cellular damage, such as oxidative damage, inflammatory responses, DNA damage, cell death, or apoptosis. Moreover, the normal function of the human body’s respiratory, cardiovascular, immune, digestive, urinary, and nervous systems may also be impacted by these fuel emissions according to cytotoxic research. Cytotoxicity of fuel combustion products is usually related to the fuel type, time, dose, and cell line used in the experiment. This review provides some ideas for the exhaust emission management of zero-carbon and carbon-neutral fuels and human health assessment. It also presents a theoretical and experimental basis for further research, including in vivo experiments.

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“…Overall, microalgae represent a very useful and important model for NMs’ risk assessment considering their high sensitivity to CNMs and the fact that they are the main producers of organic matter in water bodies, and a basic trophic level, which gives rise to all water food chains [ 38 ]. Commonly, microalgae bioassays indicate the effects of pollutants on growth rate conditions, chlorophyll and protein content, DNA damage, oxidative stress, enzyme activity, membrane polarization, cell size changes, and other biochemical or morphological changes in the cells [ 39 ]. CNMs mostly affect microalgae cells through mechanical damage, accumulation in the cell membranes, and subsequent shading effect, which causes photosynthetic efficiency reduction and growth inhibition of microalgae [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicity and Biotransformation of Carbon-Based Nanomaterials in Marine Microalgae Heterosigma akashiwo

Pikula

Johari

Santos-Oliveira

et al. 2023

IJMS

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This work is related to the environmental toxicology risk assessment and evaluation of the possible transformation of carbon-based nanomaterials (CNMs) after contact with marine microalgae. The materials used in the study represent common and widely applied multi-walled carbon nanotubes (CNTs), fullerene (C60), graphene (Gr), and graphene oxide (GrO). The toxicity was evaluated as growth rate inhibition, esterase activity, membrane potential, and reactive oxygen species generation changes. The measurement was performed with flow cytometry after 3, 24, 96 h, and 7 days. The biotransformation of nanomaterials was evaluated after 7 days of microalgae cultivation with CNMs by FTIR and Raman spectroscopy. The calculated toxic level (EC50 in mg/L, 96 h) of used CNMs reduced in the following order: CNTs (18.98) > GrO (76.77) > Gr (159.40) > C60 (414.0). Oxidative stress and membrane depolarization were the main toxic action of CNTs and GrO. At the same time, Gr and C60 decreased the toxic action with time and had no negative impact on microalgae after 7 days of exposure even at the concentration of 125 mg/L. Moreover, C60 and Gr after 7 days of contact with microalgae cells obtained structural deformations.

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Methodological advances and future directions of microalgal bioassays for evaluation of potential toxicity in environmental samples: A review

Cited by 17 publications

References 146 publications

Biological impact of ocean alkalinity enhancement of magnesium hydroxide on marine microalgae using bioassays simulating ship-based dispersion

Biological impact of ocean alkalinity enhancement of magnesium hydroxide on marine microalgae using bioassays simulating ship-based dispersion

Zero-Carbon and Carbon-Neutral Fuels: A Review of Combustion Products and Cytotoxicity

Toxicity and Biotransformation of Carbon-Based Nanomaterials in Marine Microalgae Heterosigma akashiwo

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