Methodological Approach to the Elaboration of the Analytical Procedure of the Antioxidant Activity Determination of the Schisandra chinensis (Turcz) Baill. Extracts

Hudz, Nataliia

doi:10.15414/ainhlq.2021.0024

Cited by 3 publications

(2 citation statements)

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“…Extracts from Rh. tomentosum shoots were obtained using a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material [28,[57][58][59]. Extracts were prepared using different concentrations of hydroalcoholic mixture and purified water to determine the effect of ethanol concentration on the extraction of flavonoids in the form of aglycones and glycosides since glycosidic and aglyconic forms of flavonoids have different solubility [27,60,61].…”

Section: Extracts Preparationmentioning

confidence: 99%

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Kukhtenko,

Bevz,

Konechnyi

et al. 2024

Molecules

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In the literature, the chemical composition of Rhododendron tomentosum is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from Rh. tomentosum shoots using ethanol of various concentrations and purified water as an extractant. Extracts from Rh. tomentosum were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. Using the method of high-performance thin-layer chromatography in a system with solvents ethyl acetate/formic acid/water (15:1:1), the following substances have been separated and identified in all the extracts obtained: rutin, hyperoside, quercetin, and chlorogenic acid. The total polyphenol content (TPC) and total flavonoid content (TFC) were estimated using spectrophotometric methods involving the Folin-Ciocalteu (F-C) reagent and the complexation reaction with aluminum chloride, respectively. A correlation analysis was conducted between antioxidant activity and the polyphenolic substance content. Following the DPPH assay, regression analysis shows that phenolic compounds contribute to about 80% (r2 = 0.8028, p < 0.05) of radical scavenging properties in the extract of Rh. tomentosum. The extract of Rh. tomentosum obtained by ethanol 30% inhibits the growth of test cultures of microorganisms in 1:1 and 1:2 dilutions of the clinical strains #211 Staphylococcus aureus and #222 Enterococcus spp. and the reference strain Pseudomonas aeruginosa ATCC 10145.

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Section: Extracts Preparationmentioning

confidence: 99%

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Kukhtenko,

Bevz,

Konechnyi

et al. 2024

Molecules

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“…It can be explained by that the coefficients of correlation are significantly decreased at 40 min compared to 30 min. Moreover, such a time was also established for the extracts of Schizandra chinensis according to the same analytical technique [37].…”

Section: T a B L E 1 The Content And Purity Of The Tested Sample Of C...mentioning

confidence: 99%

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

Hudz¹,

Turkina²,

Yezerska³

et al. 2022

Ukr.Biochem.J.

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The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry mass and its spectrum characteristics. The two clear absorption maxima were detected in the spectrum of C-PC at the wavelengths of 277 and 619 nm. The content of C-PC in the dry powder form was in the range of 7.25% to 9.30% depending on its concentration in the solution and type of spectrophotometer. Furthermore, a purity factor of 1.5 was calculated, which indicated the food qualification of the obtained biomass of C-PC. Finally, the analytical procedure for studying the pro- and anti-oxidant activity of C-PC was developed and the antioxidant activity of C-PC was measured for the available markers. It was revealed that C-PC has dual properties (pro- and anti-oxidant ones) depending on its concentration, more exactly, its content in reaction mixtures with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The following issues were resolved during the research: the concentration of ethanol in the DPPH solution was chosen in order to avoid precipitation of proteins in the reaction mixtures (50%); the ratio of the solution of C-PC to the DPPH solution was selected; the selected concentrations of the markers for the construction of their calibration curves were chosen for quercetin and for rutin. The antioxidant activity of the obtained C-PC sample was determined. Keywords: antioxidant activity, C-phycocyanin, cyanobacteria, DPPH, quercetin, rutin

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Methodical Approach to the Determination of the Antioxidant Activity of the Echinacea Purpurea and Monarda Fistulosa Tinctures as a Quality Criterion of the Development of Their Composition and Laboratory Technology

Yezerska

Hudz

Kobylinska

et al. 2022

AML

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Aim. The aim of the study was to develop the methodical approach to determine the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea, as well as to develop their technology in laboratory conditions. Materials and Methods. The following methods were used: analysis, synthesis, systematization, and comparison for processing of published scientific data on antioxidant activity; method for measuring the particle size of raw herbal materials; maceration and remaceration methods for obtaining the tested tinctures; DPPH test for the valuation of the antioxidant activity of the developed tinctures. Results and Discussion. The methodical approach to determining the antioxidant activity of the tinctures of Monarda fistulosa herb and flowers and roots of Echinacea purpurea was elaborated, the essence of which consisted in the selection of the appropriate dilution of the tinctures. Tinctures were prepared with the help of maceration or/and remaceration in laboratory conditions. The ratios of herbal raw materials (HRM) to the final tincture were close to ratios that are widely employed in the pharmaceutical industry, namely 1 to 5 and 1 to 10. The coefficients of alcohol absorption for the roots of Echinacea purpurea (size 2-5 mm), flowers of Echinacea purpurea (size 1-3 mm), herb of Monarda fistulosa (size 0.5-3 mm) were measured. They were measured as 1.2, 2.25, and 5.0 ml/g, respectively, for 70% ethanol. The studies revealed that Echinacea purpurea tinctures are a valuable source of antioxidant compounds. The antioxidant activity of these tinctures was 254.8-815.8 mg rutin-equivalents in 1 L of the tinctures or 1.12-4.43 mg rutin-equivalents in 1 g of the HRM depending on the part of the plant, particle size and extraction type. The antioxidant activity of the tinctures of the Monarda fistulosa herb was equal to 2203.6 mg eq-rutin/L and 20.3 mg eq-rutin/g for the tincture and HRM at a ratio of 1 to 9.5 and 2119.4 mg eq-rutin/L and 9.7 mg eq-rutin/g for the tincture at a ratio of 1 to 4.5. Conclusions. The approach to the determination of the antioxidant activity of the tested tinctures was elaborated, namely the dilutions of the tinctures were established for the analytical procedure of the determination of the antioxidant activity. Our studies demonstrated that tinctures of Echinacea purpurea contained compounds with antioxidant activity. The tinctures of Monarda fistulosa herb are very rich in compounds with antioxidant properties. The laboratory technology of six tinctures was elaborated. Further studies will be directed at laboratory studies on microorganisms and animals.

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Methodological Approach to the Elaboration of the Analytical Procedure of the Antioxidant Activity Determination of the Schisandra chinensis (Turcz) Baill. Extracts

Cited by 3 publications

References 28 publications

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithuania)

Methodical Approach to the Determination of the Antioxidant Activity of the Echinacea Purpurea and Monarda Fistulosa Tinctures as a Quality Criterion of the Development of Their Composition and Laboratory Technology

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