The article shows that in the conditions of Vietnamese fields to prevent a decrease in the enzymes activity in reservoir conditions due to high salinity and temperature, it is necessary to include chelate compounds limiting the metal ions influence. The results of the EDTA-chelate experiment showed that at EDTA concentrations from 2.0 to 0.8%, the turbidity of the enzyme solution decreased during the testing time, demonstrating the positive effect of EDTA with enzymes in a salt water environment. The results of a study comparing changes in the enzyme and surfactant solutions surface tension showed that the addition of 1% stabilizer EDTA, 1% surfactant alpha-olefinsulfonate to the enzyme solution increases the activity of the system. Based on the experimental data obtained using the Modde 5.0 software, the optimal concentrations of enzyme and surfactant solutions for the minimum value of surface tension were determined. The optimal effect of the minimum surface tension value is 1.735 mN/m with components was obtained at a ratio of components enzyme:surfactant=0.477:0.3. Based on the results of optimization, the component composition of the system was obtained: microbial product of enzyme origin is 50%; Surfactant is 30%; stabilizer is 1.0%; microorganisms inhibitor is 0.5%. Keywords: enzyme; surfactants; bottomhole formation zone; chelate compounds; surface tension.