Methodological approaches for studying the microbial ecology of drinking water distribution systems

Douterelo, Isabel; Boxall, Joby; Deines, Peter; Sekar, Raju; Fish, Katherine E.; Biggs, Catherine A.

doi:10.1016/j.watres.2014.07.008

Cited by 218 publications

(162 citation statements)

References 218 publications

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“…In the recent years, next-generation sequencing (NGS) technologies have been developed and utilized in studies to understand biofilms' cellular activities, relationship between essential genes in biofilm formation and biofilm community structures (Franklin et al, 2015). NGS technologies can identify specific bacterial biofilms in clinical (Huebinger et Grumaz et al, 2016Franklin et al, 2015Grumaz et al, 2016Barzon et al, 2011Douterelo et al, 2014Metzker, 2010 Fluorescence-based microscopy techniques A number of advanced fluorescence-based microscopy techniques namely confocal laser scanning microscopy (CLSM) and fluorescence microscopy in combination with automatic counting software are applied in biofilm cells quantification (Drago et al, 2016; Freitas et al, 2014) due to their superiority over the CFU count technique (Freitas et al, 2014). A report by Drago et al…”

Section: Article Infomentioning

confidence: 99%

Bacterial Biofilm Cells Quantification Techniques: Where Is Consensus in Over Two Decades?

Olwal¹,

Ochiel²,

Onyango³

et al. 2017

J microb biotech food sci

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It is widely accepted that bacterial biofilms are overly resistant to antibiotics, host immunity and disinfectants. Biofilms develop on various food-processing surfaces hence pose major risks in food industries. Biofilms serve as protective niches for pathogens in food and water thus enhance transmission of food borne pathogens. Furthermore, biofilms are implicated in medical implant infections. The serious problems associated with bacterial biofilms in food, biomedical and environmental fields have stimulated active research on biofilms for over two decades. Biofilm cells quantification is important in many research applications especially in anti-biofilm efficacy studies and quality controls in many industries. However, to date there is no consensus on which technique is most suitable for quantifying bacterial biofilm cells. This apparent lack of a standard technique has hindered effective comparison of results from different bacterial biofilm studies since each technique has a unique readout. Furthermore, it appears that the choice of a biofilm cells quantification technique is largely a matter of convenience and availability of a technique. This may introduce biasness. Consequently, this review critically assesses the availability, suitability and limitations of different techniques for quantifying biofilm cells. This could inform better control and management of bacterial biofilms in environmental and clinical settings.

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Section: Article Infomentioning

confidence: 99%

Bacterial Biofilm Cells Quantification Techniques: Where Is Consensus in Over Two Decades?

Olwal¹,

Ochiel²,

Onyango³

et al. 2017

J microb biotech food sci

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“…in hot water is also related to the long incubation time. It seems that ATP is a useful indicator for routine control [50,51].…”

Section: Usable Hot Watermentioning

confidence: 99%

Using ATP tests for assessment of hygiene risks

Pistelok

Pohl

Stuczyński

et al. 2016

Ecological Chemistry and Engineering S

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Adenosine-5'-triphosphate (ATP) is a high-energy molecule playing a role of universal energy carrier used in cell metabolism. Since ATP is present in both prokaryotic and eukaryotic cells, this coenzyme can be used to determine a hygiene state. During last decade there has been a significant growth of practical applications of ATP measurements. ATP tests are commonly used for an assessment of hygienic conditions in food industry, residential buildings and hotels. In comparison with traditional and time-consuming laborious microbial analysis the measurement of ATP provides a quick indicative on-site data, characterizing microbial and organic contamination to support decision-making with regards to adequate corrective and preventive actions. This paper demonstrates applications of ATP in practice and, particularly, in water treatment technology and wastewater processing. The review highlights opportunities for using the test for the optimization of water treatment facilities and pipe networks functioning in order to assess wastewater toxicity and wastewater treatment process efficiency. Other areas of application are also addressed. The review of literature indicates that ATP used in different areas of industry can be considered as a diagnostic method -complement the classical methods -useful for identification of hygiene risks as well as a tool for a real-time management of health and environmental risks.

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“…Sin embargo, el actual milenio trajo consigo el entendimiento mundial de que la misma puede ser inagotable, siendo su progresiva y marcada dirección hacia la escasez uno de los principales retos geopolíticos planetarios a encarar con respecto a un manejo más integral de los recursos hídricos, conceptualizados en una gestión mucho más eficiente, donde se contemple la no contaminación, su ahorro y reutilización (Valencia et al, 2012;Douterelo et al, 2014;Petrie et al, 2015;Hu et al, 2016). En este aspecto, es común que en muchas partes del mundo se empleen sistemas de recuperación como una manera efectiva de suplir los reservorios de agua potable (Gerrity et al, 2013;Rice y Westerhoff, 2014).…”

Section: Introductionunclassified

Diagnóstico del agua utilizada en las procesadoras de café del Municipio Caripe – Estado Monagas, Venezuela.// Diagnosis of the water used in coffee processors of the Caripe Municipality - Monagas State, Venezuela

Acuña

Acuña²,

Martı́nez³

et al. 2017

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ResumenLatinoamérica es la región de mayor producción de café del mundo y en Venezuela Monagas conforma uno de los estados productores en la zona del Municipio Caripe, el cual se surte de cuenca hidrográfica siendo el río Caripe su principal recurso hídrico. La finalidad del presente trabajo consistió en realizar un primer diagnóstico para la calidad del agua utilizada durante el proceso de beneficiado húmedo del café en las pocas procesadoras que aún operan en el Municipio Caripe, considerando específicamente las ubicadas en las Fincas: "Agropecuaria Cantarrana", "Gualberto", "Cocollar", "Lisieux" y "Las Acacias"; a manera de determinar si los parámetros evaluados cumplen con los valores permitidos para la Legislación Venezolana vigente. En base a los resultados obtenidos, en general se observó que todos los parámetros evaluados cumplen con los límites permitidos en cuanto a su calidad, a excepción del análisis microbiológico relacionado con los niveles de coliformes totales cumplido sólo para la beneficiadora "Cantarrana"; mientras que las demás productoras incumplen con este parámetro de calidad siendo bastante evidente en el caso de la procesadora "Cocollar", cuya agua presentó los más altos niveles para estos patógenos (240 para el índice de Número Más Probable/100 mL), que son indicativos de contaminación microbiológica.Palabras Clave: Recurso hídrico, calidad de agua, beneficiado del café, Municipio Caripe. AbstractLatin America is the region with the highest coffee production in the world, and Monagas is one of the producing states in the area of the Caripe Municipality, Venezuela, which is supplied with a hydrographic basin, being the Caripe River its main water resource. The purpose of this study was to conduct a preliminary assessment to the quality of water used during the wet coffee processing in the few processors thet still operating in the Caripe Municipality. It was considered specifically those located in "Agropecuaria Cantarrana", "Gualberto", "Cocollar", "Lisieux", and "Las Acacias" farms, in order to determine whether the evaluated parameters meet the allowed values for the current Venezuelan Legislation. Based on the results obtained, in general it was observed that all parameters evaluated comply with the permitted limits in terms of quality, except for microbiological analysis related to the levels of total coliforms fulfilled only for "Cantarrana" processor. While other producers fail to comply with this quality parameter, being quite evident in the case of the "Cocollar" processor whose water had the highest levels for these pathogens (240 for the Most Probable Number/100 mL index), which are indicative of microbiological contamination.

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Methodological approaches for studying the microbial ecology of drinking water distribution systems

Cited by 218 publications

References 218 publications

Bacterial Biofilm Cells Quantification Techniques: Where Is Consensus in Over Two Decades?

Bacterial Biofilm Cells Quantification Techniques: Where Is Consensus in Over Two Decades?

Using ATP tests for assessment of hygiene risks

Diagnóstico del agua utilizada en las procesadoras de café del Municipio Caripe – Estado Monagas, Venezuela.// Diagnosis of the water used in coffee processors of the Caripe Municipality - Monagas State, Venezuela

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