Methodological aspects about in vitro evaluation of antioxidant properties

Magalhães, Luís M.; Segundo, Marcela A.; Reis, Salette; Lima, José L.F.C.

doi:10.1016/j.aca.2008.02.047

Cited by 626 publications

(468 citation statements)

References 218 publications

(173 reference statements)

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“…Em meios aquosos a solubilidade é bem menor e pode interferir na avaliação da capacidade antioxidante, tornando o radical DPPH• pouco acessível para a reação com as amostras antioxidantes, afetando assim a transferência de elétron ou de hidrogênio (Karadag et al, 2009;Magalhães et al, 2008 (Magalhães et al, 2008;Gülçin, 2012;Cieśla et al, 2012). No trabalho desenvolvido por Ozcelik; Lee; Min (2003), foi observado que há uma perda da absorbância da reação entre a solução de DPPH• e o antioxidante, em torno de 20 a 35% na presença de luz.…”

Section: Método Do Dpph•unclassified

“…O método DPPH• não é apropriado para medir a capacidade antioxidante de plasma e soro humano, porque as proteínas podem precipitar no meio da reação em solução alcoólica (Karadag et al, 2009). No entanto, o método DPPH• tem aplicação em soro desproteinado, sendo o estudo antioxidante em soro humano uma ferramenta importante (Chrzczanowicz et al, 2008;Magalhães et al, 2008;Martinez et al, 2006;Rysz et al, 2010).…”

Section: Método Do Dpph•unclassified

“…O valor da absorbância pode ser ainda interpolado em uma curva de dose-resposta de um antioxidante padrão, como ácido ascórbico ou Trolox, ácido tânico e ácido gálico e os resultados são expressos como concentração equivalente (Kedare;Singh, 2011;Magalhães et al, 2008;Arnao, 2000).…”

Section: N T E R P R E T a ç ã O D O S R E S U L T A D O S Experimeunclassified

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Determinação da capacidade antioxidante de produtos naturais in vitro pelo método do DPPH•: estudo de revisão

Oliveira

2015

Rev. bras. plantas med.

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RESUMO:Os antioxidantes podem ser de grande benefício para a melhoria da qualidade de vida, já que eles têm a capacidade de proteger um organismo dos danos causados pelos radicais livres, prevenindo ou adiando o início de várias doenças. Uma das técnicas atualmente utilizada para detectar a presença de compostos antioxidantes, é um método baseado na eliminação do radical livre estável 1,1-difenil-2-picrilhidrazil (DPPH•). Este método é considerado fácil, preciso, rápido, simples, e econômico, sendo adequado para a determinação da capacidade antioxidante de substâncias puras e misturas. O objetivo deste artigo de revisão é fornecer informações sobre esse método. A pesquisa foi realizada usando o termo radical DPPH•, capacidade antioxidante-DPPH• e método DPPH• em periódicos, tais como: Pubmed, Wiley Online Library, ACS Publications, SpringerLink e ScinceDirect até janeiro de 2014. Palavras-chave: Antioxidantes, método DPPH•, capacidade antioxidante.ABSTRACT: Determination in vitro of the antioxidant capacity of natural products by the DPPH•method: review study. Antioxidants can be of great benefit to improve the quality of life, since they have the ability to protect an organism from damage caused by free radicals, preventing or delaying the onset of various diseases. One of the techniques currently used to detect the presence of antioxidant compounds is a method based on the elimination of the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH•). This method is considered easy, accurate, rapid, simple and inexpensive, and it is suitable for the determination of the antioxidant capacity of pure substances and blends.

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Section: Método Do Dpph•unclassified

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Determinação da capacidade antioxidante de produtos naturais in vitro pelo método do DPPH•: estudo de revisão

Oliveira

2015

Rev. bras. plantas med.

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“…5A), and used in vitro as a ROS scavenger. To determine the in vitro efficiency of ASR1 as a ROS scavenger as well as its ability to convert H 2 O 2 to H 2 O as compared with that of catalase (Magalhaes et al, 2008), H 2 O 2 levels were evaluated using the FOX reagent. As shown in Fig.…”

Section: Activation Of Cell Rescue Proteins In Asr1-expressing Yeast mentioning

confidence: 99%

Rice ASR1 Protein with Reactive Oxygen Species Scavenging and Chaperone-like Activities Enhances Acquired Tolerance to Abiotic Stresses in Saccharomyces cerevisiae

Kim

Yoon

2012

Molecules and Cells

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Abscisic acid stress ripening (ASR1) protein is a small hydrophilic, low molecular weight, and stress-specific plant protein. The gene coding region of ASR1 protein, which is induced under high salinity in rice (Oryza sativa Ilmi), was cloned into a yeast expression vector pVTU260 and transformed into yeast cells. Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide (H 2 O 2 ), high salinity (NaCl), heat shock, menadione, copper sulfate, sulfuric acid, lactic acid, salicylic acid, and also high concentration of ethanol. In particular, the expression of metabolic enzymes (Fba1p, Pgk1p, Eno2p, Tpi1p, and Adh1p), antioxidant enzyme (Ahp1p), molecular chaperone (Ssb1p), and pyrimidine biosynthesis-related enzyme (Ura1p) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells. All of these enzymes contribute to an alleviated redox state to H 2 O 2 -induced oxidative stress. In the in vitro assay, the purified ASR1 protein was able to scavenge ROS by converting H 2 O 2 to H 2 O. Taken together, these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells.

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“…While the FRAP assay quantifies the total concentration of redox-active compounds, 13 the amperometric method at +0.5 V has been demonstrated to selectively discriminate those compounds having effective antioxidant power. [14][15][16] Antioxidant capacity (Table 1) of the bilberries from the region of Velingrad was 15 and 20% higher than that of the Troyan region sample for the FRAP and EC +0.5 V values, respectively.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant capacity and anthocyanin composition of Bulgarian bilberry (Vaccinium myrtillus L.) fruits

Georgieva¹

2018

MOJFPT

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Antioxidant capacity of Bulgarian bilberry (Vaccinium myrtillus L.) fruits from two different picking regions, Velingrad and Troyan, was studied using the FRAP (ferric reducing antioxidant power) assay and amperometric method. In addition, the anthocyanin composition was characterised by high performance liquid chromatography -diode array detection. Antioxidant capacity of the bilberries from the region of Velingrad was 15 and 20% higher than that of the Troyan region sample for the FRAP and EC +0.5 V values, respectively. Among the 12 individual anthocyanins identified, delphinidin glycosides were the predominant compounds. In accordance with the content of total anthocyanins, higher concentrations (20-64%) of individual compounds were observed for the bilberries from the region of Velingrad. The results obtained demonstrate that the environmental conditions within the picking regions have significant effects on the antioxidant capacity and anthocyanin content of Bulgarian bilberry fruits, which may affect their applications as functional foods or ingredients. Keywords:

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Methodological aspects about in vitro evaluation of antioxidant properties

Cited by 626 publications

References 218 publications

Determinação da capacidade antioxidante de produtos naturais in vitro pelo método do DPPH•: estudo de revisão

Determinação da capacidade antioxidante de produtos naturais in vitro pelo método do DPPH•: estudo de revisão

Rice ASR1 Protein with Reactive Oxygen Species Scavenging and Chaperone-like Activities Enhances Acquired Tolerance to Abiotic Stresses in Saccharomyces cerevisiae

Antioxidant capacity and anthocyanin composition of Bulgarian bilberry (Vaccinium myrtillus L.) fruits

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