Methodological aspects of moss sample preparation. Effects of freezing and duration of washing on the cellular distribution of elements in Fontinalis squamosa Hedw.

Vázquez, M.D.; Villares, Ramón; Carballeira, A.

doi:10.1016/j.ecolind.2015.04.014

Cited by 7 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, if the storage method weakened the cell walls, cleaning the samples might wash away part of the pollutants accumulated in their interior. Vázquez et al (2015) found that an increase in washing time can be related to a reduction in the total concentration of more mobile macronutrients in samples of F. antipyretica , while the effect on trace metals was minimal. On the other hand, an increase in the concentration of certain elements after washing has also been found in terrestrial mosses, probably because of the solubilization of particles deposited on the surface that ended up incorporated into the moss (Fernández et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…For example, although certain species of aquatic bryophytes, such as F. antipyretica , can spend some time emerged during the dry season (Cruz de Carvalho et al, 2011), it is known that drying can alter the permeability of cell membranes in mosses, allowing the elements inside the cell to move outside (Brown & Brumelis, 1996; Brown & Buck, 1979). Freezing can also break or alter the cell membranes, favoring the release of intracellular elements (Vázquez et al, 2015). Therefore, keeping the samples humid in a refrigerator seems to be the least aggressive method.…”

Section: Introductionmentioning

confidence: 99%

“…Wehr et al (1983) compared samples of two species of aquatic moss dried at room temperature with others stored in a refrigerator (although only overnight). Vázquez et al (2015), in a study focused on the cellular distribution of several elements, compared their concentrations in different cellular locations in frozen (−20°C for 7 days) and fresh (4°C < 24 h) Fontinalis squamosa samples. By contrast, storage effects on trace element concentration have received more interest in biomonitoring with terrestrial mosses (e.g., Aboal et al, 2008; Dołęgowska & Migaszewski, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of the storage procedure on the trace element content measured in the aquatic moss Fontinalis antipyretica Hedw

Villares

Vázquez

Real

2023

Water Environment Research

Self Cite

View full text Add to dashboard Cite

Aquatic bryophytes have been used as pollution biomonitors for decades. Despite this, sample collection and preparation methods have not been standardized, which makes it difficult to compare the results of different studies. Most times, the samples have to be stored before processing, for example, when many of them are collected in a short time, as occurs in extensive pollution studies. Storage must be done in a way that does not change the pollutant concentrations in the samples. We studied whether the concentrations of Al, As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, and Zn in the aquatic moss Fontinalis antipyretica were affected by three storage procedures: dry at room temperature, fresh (in refrigerator at 6°C), and frozen at −20°C. In addition, we evaluated whether the subsequent washing of the samples affected the concentrations of these elements differently depending on the storage method. Our results showed that the three methods were, in general, adequate since the concentrations did not change, and we did not observe differences between washed and unwashed samples either. Since the simplest method is refrigeration, we concluded that this is the best of them. However, the concentrations of Hg increased steadily over time in the fresh material, probably because of redistribution after volatilization from the basal parts of the mosses. We believe that the respiration of the plants lowered the concentrations of oxygen inside the hermetically sealed bags containing the samples, thus promoting the reduction of the Hg and its posterior volatilization and redistribution. We did not observe interactions between the storage method and the posterior washing of the samples. Practitioner Points We studied the influence of storage procedure on element content in aquatic mosses. The procedures were as follows: dried at room temperature, stored in refrigerator, and frozen. The procedures did not change the element contents, except for Hg in refrigerator. All the procedures seemed suitable, but refrigeration was the simplest one.

show abstract

Section: Introductionmentioning

confidence: 99%