2002
DOI: 10.1161/01.res.0000014802.05780.ae
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Methodological Considerations on the Detection of 3-Nitrotyrosine in the Cardiovascular System

Abstract: Reactive nitrogen species (RNS; eg, • NO,• NO 2 , ONOO Ϫ , NO 2 Cl) react preferably with tyrosine (Tyr) and protein-associated tyrosine (TyrProt) to form 3-nitrotyrosine, ie NO 2 Tyr and NO 2 TyrProt, respectively. 1 Therefore, detection of NO 2 Tyr and/or NO 2 TyrProt provides evidence for generation of RNS rather than specifically peroxynitrite (ONOO Ϫ ). 1 Besides this difficulty, Tarpey and Fridovich 2 have recently discussed, in an article published in Circulation Research, the problematic measurement of… Show more

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Cited by 35 publications
(21 citation statements)
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“…Despite significant methodological advances over the last years, the major obstacle in assessing the biological significance of RSNOs lies in the lack of their reliable measurement in complex biological matrices [7 -9]. In fact, the range of basal RSNO levels reported in the various studies covers more than three orders of magnitude [10]. A lot of artefacts are due to the procedure used to cleave RSNO into NO and oxidized thiol.…”
Section: Introductionmentioning
confidence: 99%
“…Despite significant methodological advances over the last years, the major obstacle in assessing the biological significance of RSNOs lies in the lack of their reliable measurement in complex biological matrices [7 -9]. In fact, the range of basal RSNO levels reported in the various studies covers more than three orders of magnitude [10]. A lot of artefacts are due to the procedure used to cleave RSNO into NO and oxidized thiol.…”
Section: Introductionmentioning
confidence: 99%
“…Artifactual formation of 3-nitrotyrosine during sample setup, insufficient detection sensitivity and lack of specificity are the most serious and widely recognized analytical shortcomings (discussed in Refs. [3][4][5][6]). …”
Section: Introductionmentioning
confidence: 99%
“…Much of the current controversy over the presence and role of S-nitrosothiols (RSNOs), 5 in plasma and red blood cells (RBCs), has been attributed to differences in analytical approaches and their ability to properly detect nitric oxide (NO)-derived products (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). These include factors that affect sensitivity, specificity and selectivity for distinct NO-related species and formation or destruction of products during sample preparation that can lead to an over-or underestimation of what is being detected.…”
mentioning
confidence: 99%