Methodology An efficient approach to identify Ginkgo biloba cultivars by using random amplified polymorphic DNA markers with a manual cultivar identification diagram strategy

Abstract

ABSTRACT. Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that consisted of polymorphic bands produced from different RAPD primers was reported. To check the reliability and efficiency of the cultivar identification diagram, 5 randomly chosen cultivars were further tested, and the workability of the diagram was verified. This new approach will be very helpful for Ginkgo cultivar discrimination and protection, and will also be beneficial for the nursery industry for early identification of Ginkgo seedlings.

Cultural Extension of Ginkgo biloba L. in Slovakia

“…On the importance of recognizing genetic diversity of Ginkgo biloba populations pointed out several studies focused on applying different molecular markers as genomic microsatellites (Yan et al, 2006;Yan et al, 2009;Li et al, 2009;Xie et al, 2013), RAPD (Fan et al, 2004;Li et al, 2013;Mei et al, 2014), ISSR (Mei et al, 2014), RFLP (Shen et al, 2005) and microsatellites (Xu et al, 2015) markers based on chloroplast DNA and microRNA-based markers (Ražná et al, 2020).…”

Ecologically conditioned imprinting of miRNA-based profiles of Ginkgo biloba L. growing in Slovakia

“…Molecular DNA markers are an integral part of the evaluation process for assessing the genomes in significant plant genetic resources (Li et al, 2013). Markers of miRNA have a great potential in terms of the differentiation of closely related plant species, analysis of their genetic diversity and genetic mapping.…”

“…The ginkgo genome is of high scientific interest for its exclusive properties (Lin et al, 2011). The genomic research on G. biloba includes; (a) the identification of markers for sex identification -to support field planting of ginkgo (Liao et al, 2009;Yang et al, 2005), (b) cultivar identification -to contribute to the conservation, genetic diversity and mating patterns (Fan et al, 2004;Li and Zhang, 2013;Mei et al, 2014;Yan et al, 2006;Yan et al, 2009), (c) the high-throughput genomic research (Han et al, 2015;He et al, 2015;Lin et al, 2011) and (d) the comparison of the genomes of the species of G. biloba identified in Slovakia and abroad, characterization of morphological gender differences, and peculiarities within the species (Ražná et al, 2014;Ražná and Hrubík, 2016). Šmarda et al (2016) observed the polyploidy of the ginkgo genome, which is quite unusual in gymnosperms.…”