Methodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic 18O-phosphate species

Abstract

Phosphorus (P) fertilizers are crucial to achieve peak productivity in agricultural systems. However, the fate of P fertilizers via microorganism incorporation and the exchange processes between soil pools is not well understood. 18 Oxygen-labelled phosphate (18 O-P i) can be tracked as it cycles through soil systems. Our study describes biological and geochemical P dynamics using a tandem mass spectrometry (MS/MS) method for the absolute quantification of 18 O-P i. Soil microcosms underwent three treatments: (i) 18 O-P i , (ii) unlabelled phosphate (16 O-P i) or (iii) Milli-Q control, dissolved in a bio-stimulatory solution. During a 6-week series the microcosms were sampled to measure P by Hedley sequential fractionation and DNA extraction samples digested to 3 0-deoxynucleoside 5 0-monophosphates (dNMP). A MS/MS attached to a HPLC analyzed each P-species through collisioninduced dissociation. The resin-extractable and bicarbonate 18 O-P i and 16 O-P i fractions displayed similar precipitation and adsorption-desorption trends. Biotic activity measured in the NaOH and dNMP fractions rapidly delabelled 18 O-P i ; however, the MS/MS measured some 18 O that remained between the P backbone and deoxyribose sugars. After 6 weeks, the 18 O-P i had not reached the HCl soil pool, highlighting the long-term nature of P movement. Our methodology improves on previous isotopic tracking methods as endogenous P does not dilute the system, unlike 32 P techniques, and measured total P is not a ratio, dissimilar from natural abundance techniques. Measuring 18 O-P i using MS/MS provides information to enhance land sustainability and stewardship practices regardless of soil type by understanding both the inorganic movement of P fertilizers and the dynamic P pool in microbial DNA.

Oxygen isotope ratios of phosphates in the soil‐plant system: Limitations and future developments

“…Recently, the use of LC MS/MS (Schryer et al, 2020) and Orbitrap HRMS (high-resolution mass spectrometer (Hilkert et al, 2021;Neubauer et al, 2020)) for the analysis of oxyanions have been proposed for the analysis of δ 18 O p . If successful, these two approaches might expedite the analytical procedure and time, since the preparation requirements for the samples could be reduced.…”