Methodology-Dependent Variations in Reticulocyte Counts Using a Manual and Two Different Flow Cytometric Procedures

Houte, A J van; Bartels, P. C. M.; Schoorl, Marianne; Mulder, Clara H.

doi:10.1515/cclm.1994.32.11.859

Cited by 8 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a later study, van Houte et al (78) found a poor correlation between the microscopic count and both flow cytometric methods (FACScan r = 0.61; Sysmex r = 0.57), but the correlation between the flow cytometric methods was satisfactory (r = 0.79). Their reference ranges for the reticulocyte count, corresponding with the 2.5th and 97.5th percentile, were as follows: microscopic (8-30/1000), FACScan (11-27/1000) and Sysmex (8-18/1000).…”

Section: Technical Considerationsmentioning

confidence: 91%

Reticulocytes and reticulocyte enumeration

Riley

Ben‐Ezra

Goel

et al. 2001

Clinical Laboratory Analysis

105

View full text Add to dashboard Cite

Section: Technical Considerationsmentioning

confidence: 91%

Reticulocytes and reticulocyte enumeration

Riley

Ben‐Ezra

Goel

et al. 2001

Clinical Laboratory Analysis

105

View full text Add to dashboard Cite

“…The first paper in CCLM was in 1994, when flow cytometric methods had just become available for routine use. Compared to manual reticulocyte counting, flow cytometry was found to be much more precise and enabled improved reticulocyte enumeration of abnormally low counts [39] . A recent review article confirmed the superiority of automated, flow cytometric methods, in particular methods using fluorescent dyes [40] .…”

Section: Major Categories Of Lh Papers In Chronological Ordermentioning

confidence: 99%

Laboratory hematology in the history of Clinical Chemistry and Laboratory Medicine

Hoffmann

2012

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

Background: For the occasion of the 50th anniversary of the journal Clinical Chemistry and Laboratory Medicine ( CCLM ), an historic overview of papers that the journal has published in the field of laboratory hematology (LH) is presented. Methods : All past volumes of CCLM were screened for papers on LH and these were categorized. Bibliographic data of these papers were also analyzed. Results: CCLM published in total 387 LH papers. The absolute number of LH papers published annually showed a significant increase over the years since 1985. Also the share of LH papers demonstrated a steady increase (overall mean 5 % , but mean 8 % over the past 4 years). The most frequent category was coagulation and fibrinolysis (23.5 % ). Authors from Germany contributed the most LH papers to the journal (22.7 % ), followed by the Netherlands and Italy (16.3 and 13.2 % , respectively). Recent citation data indicated that other publications cited LH review papers much more frequently than other types of papers. Conclusions: The history of the journal reflects the emergence and development of laboratory hematology as a separate discipline of laboratory medicine.

show abstract

“…The development of flow cytometric methods dedicated to reticulocyte count proved a suitable alternative to light microscopy for laboratories performing high volume reticulocyte count. Flow cytometric analysis was also found to provide clinically valuable information not available by light microscopy (Hackney et al, 1989, Wallen, Higashikubo & Dethlefsen 1982, Schimenti et al, 1992van Houte et al, 1994;Laurencet, Martinez & Beris, 1997).…”

Section: S U M M a R Ymentioning

confidence: 99%

“…, 1989, Wallen, Higashikubo & Dethlefsen 1982, Schimenti et al. , 1992; van Houte et al. , 1994; Laurencet, Martinez & Beris, 1997).…”

Section: Introductionmentioning

confidence: 99%

A biparametric flow cytometry analysis for the study of reticulocyte patterns of maturation

Borrione

Spaccamiglio²,

Parisi³

et al. 2010

Int J Lab Hematology

View full text Add to dashboard Cite

Automated haematological analysers still represent the gold standard for the study of reticulocyte maturation even if this technique is based on structural properties and staining affinity rather than on functional aspects. On the contrary, flow cytometry allows the simultaneous analysis of multiple cellular characteristics including functional features. Aim was to investigate whether simultaneous analysis of different reticulocyte parameters using flow cytometry may add functional information when considering their pattern of maturation. Thirty-nine healthy donors (H) and 31 haemodialysed patients on treatment with rHuEpo (HDT) were analysed. Reticulocyte counts and their stages of maturation were studied both with ADVIA 2120 and by flow cytometry. TO/CD71 scattergraph reticulocyte analysis designed a peculiar distribution which was similar among the same group of subjects (H or HDT), but different between H and HDT. distribution of the percentage of reticulocytes in low, medium and high boxes calculated by ADVIA 2120 did not show any difference between H and HDT groups, while the analysis using flow cytometry pointed out statistically significant differences between H and HDT groups in the three boxes where the TO+/CD71+ reticulocytes were localized. The present study suggests that TO/CD71 analysis was reproducible and could detect different pattern of maturation of a particular clinical setting.

show abstract

Methodology-Dependent Variations in Reticulocyte Counts Using a Manual and Two Different Flow Cytometric Procedures

Cited by 8 publications

References 15 publications

Reticulocytes and reticulocyte enumeration

Reticulocytes and reticulocyte enumeration

Laboratory hematology in the history of Clinical Chemistry and Laboratory Medicine

A biparametric flow cytometry analysis for the study of reticulocyte patterns of maturation

Contact Info

Product

Resources

About