Methodology Studies on Detection of Aminoglycoside Residues

Tian, Yi-Fang; Chen, Guanhua; Guo, Li Xin; Guo, Xin; Mei, Xiao-Yun

doi:10.1007/s12161-014-0067-5

Cited by 28 publications

(19 citation statements)

References 73 publications

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“…Apramycin (AP), synthesized by Streptomyces tenebrarius, is an aminoglycoside antibiotic that disrupts bacterial protein synthesis by binding to the 30S ribosome of susceptible organisms (Suarez, Ellis, & Bizec, 2012;Tian, Chen, Guo, Guo, & Mei, 2015). While not approved for use in humans, AP is currently used in calves, pigs, rabbits, poultry, and lambs for the treatment of infections caused by Bordetella bronchiseptica, Brucella spp., Escherichia coli, Salmonella spp., Pasteurella multocida, Pseudomonas spp., Klebsiella aerogenes, Staphylococcus spp., and α-haemolytic Streptococci (Suarez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In several countries, programs have been established to determine both antibiotic levels and maximum permissible levels in foods (Chen et al, 2015;Faten, Azzazy, & Niessen, 2015;Gaudin, Hedou, & Verdon, 2013;Tian et al, 2015;Yin, Liu, Song, Kuang, & Xu, 2015). For example, the European Union and the United States Food and Drug Administration have established maximum residue levels (MRLs) for AP that range from 60 to 20,000 µg/kg in edible animal tissues such as muscle, liver, and kidney (EU, 2010;FDA, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Isanga

Tochi

Mukunzi

et al. 2016

Food and Agricultural Immunology

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A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzymelinked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC 50) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02-105.49%, with coefficients of variation in the range of 2.21-11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5-5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Isanga

Tochi

Mukunzi

et al. 2016

Food and Agricultural Immunology

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“…Several detection methods including immunoassays, capillary electrophoresis, high-performance liquid chromatography, gas chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were developed for aminoglycoside residue analyses with different sensitivities. However, LC-MS/MS is accepted as the most reliable confirmatory method based on its high sensitivity and accuracy [11,12] .…”

Section: Introductionmentioning

confidence: 99%

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Acaröz¹,

İnce²,

Arslan‐Acaröz³

et al. 2019

Kafkas Univ Vet Fak Derg

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The present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed for the residue analysis. The detection limit of the method was determined as 3.56 μg/kg. The highest concentrations of kanamycin were determined in the first milking after injection and mean concentration of this milking was found to be as 1473 μg/kg. Kanamycin residue in all buffalo milk samples was lower than the maximum residue limit (150 μg/kg) with the fifth milking. In addition, a monitoring study was conducted to determine whether buffalo milk marketed in Afyonkarahisar pose a public health risk regarding kanamycin residue. The results of the monitoring study showed that one (2%) of the 50 buffalo milk samples contained kanamycin at the concentration of 22.36 μg/kg. In conclusion, this study determined the persistence of kanamycin in Anatolian buffalo milks based on an LC-MS/MS method. In addition, results of the study showed that buffalo milks marketed in Afyonkarahisar Province pose a very low risk regarding kanamycin residue.Bu çalışmada, laktasyondaki Anadolu mandalarına intramüsküler olarak kanamisin enjekte edilmesini takiben sütlerindeki kanamisin kalıntısının belirlenmesi amaçlandı. Süt numunelerinin toplanması, kanamisin enjeksiyonunu takiben günde iki kez 10'uncu sağıma kadar gerçekleştirildi ve kalıntı analizi için likit kromatografisi tandem kütle spektrometresi (LC-MS/MS) kullanıldı. Metodun tespit limiti 3.56 μg/kg olarak belirlendi. En yüksek kanamisin konsantrasyonları enjeksiyondan sonraki ilk sağımda tespit edildi ve sağımdaki ortalama konsantrasyon 1473 μg/kg olarak belirlendi. Manda sütü örneklerinde kanamisin kalıntı düzeyinin beşinci sağımdan itibaren maksimum kalıntı sınırından (150 μg/kg) daha düşük olduğu belirlendi. Ayrıca, Afyonkarahisar'da pazarlanan manda sütlerinin kanamisin kalıntısı konusunda halk sağlığı açısından risk oluşturup oluşturmadığını belirlemek amacıyla bir saha taraması yapıldı. Saha taraması sonucunda 50 manda sütü örneğinden birinin (%2) 22.36 μg/kg konsantrasyonunda kanamisin içerdiği saptandı. Sonuç olarak bu çalışmada kanamisinin Anadolu manda sütlerinde kalıcılığı LC-MS/MS metodu ile belirlendi. Ayrıca, çalışmanın sonuçları Afyonkarahisar'da tüketime sunulan manda sütlerinin kanamisin kalıntısı açından çok düşük risk taşıdığını gösterdi.

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“…Chromatographic methods are commonly used to detect aminoglycoside residues in foods (Faten, Azzazy, & Niessen, 2015;Kumar, Rubies, Companyo, & Centrich, 2012;Tian, Chen, Guo, Guo, & Mei, 2015) and a liquid chromatographic method for the detection of paromomycin has been developed (Pujol-Brugués et al, 2014). However, paromomycin, like other aminoglycosides, cannot be measured by UV absorption because it lacks a chromophore and has poor chromatographic properties on reversed-phase liquid chromatography (Faten et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices

Isanga

Mukunzi

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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A monoclonal antibody (MAb) was produced against paromomycin and used in the development of an immunoassay and a lateral flow strip test for the detection of paromomycin residues in food matrices. The MAb had 25.1% cross-reactivity with neomycin, but was insensitive to other aminoglycosides. Tested under optimized conditions in 0.01 M phosphate-buffered saline, the half maximum inhibitory concentration (IC 50 ) of the MAb was 0.61 ng/ml for paromomycin and 2.43 ng/ml for neomycin, with results obtained within 90 min. The mean recoveries from spiked food matrices were within the range of 64.56-105.85% for paromomycin and 54.08-100.55% for neomycin. The strip test results for different food matrices were obtained within 5 min and showed visual detection limits of 2.5-20 ng/ml (µg/kg) for paromomycin and 10-30 ng/ml for neomycin. Therefore, the developed immunoassay and strip test can be used in food analysis for routinely monitoring not only paromomycin but also neomycin residues. ARTICLE HISTORY

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Methodology Studies on Detection of Aminoglycoside Residues

Cited by 28 publications

References 73 publications

Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices

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